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Expression of laminin binding protein (37LBP/p40) and tumor angiogenesis in lung cancer

Research Project

Project/Area Number 08457178
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionTohoku University

Principal Investigator

SATOH Ken  Tohoku University, Institute of Development, Aging and Cancer, Associate Professor, 加齢医学研究所, 助教授 (00215782)

Co-Investigator(Kenkyū-buntansha) SAIJO Yasuo  Tohoku University, Institute of Development, Aging and Cancer, Instructor, 加齢医学研究所, 助手 (10270828)
SATO Yasufumi  Tohoku University, Institute of Development, Aging and Cancer, Professor, 加齢医学研究所, 教授 (50178779)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywords37-kDa / Laminin / Receptor / Angiogenesis / Lung Cancer / Gene Therapy / Antisense RNA / ラミニンレセプター / 腫瘍血管 / マウス / アンチエンスRNA / レトロウイルスベクター
Research Abstract

Expression of the 37-kDa laminin binding protein (37LBP), a precursor protein of the 67-kDa laminin receptor, correlates well with biological aggressiveness of cancer cells. However, the detailed role of 37LBP is not fully understood. To elucidate the role playd by the 37LBP in cancer cells directly, a murine lung cancer cell line T11, in which 37LBP expression was remarkably diminished, was established by an antisense RNA expression vector. When 5x10<@D15@>D1 cells were subcutaneously inoculated into syngenic mice, the mean survival time of T11-recipient (77.0(]SY.+-。[)14.8 days) was significantly prolonged compared with that of both the parental cell line P29 (34.8(]SY.+-。[)5.5 days) and the sense RNA-transfectant T42 (33.4(]SY.+-。[)4.2 days), (p<0.001). Immunohistochemistry of inoculated tumors of the same size demonstrated that microvessel density in T11 was significantly lower than that observed in both P29 and T42 (p<0.05). It was also shown that T11 cells cultured in a conditioned medium exhibited signiflcantly reduced proliferation (p<0.01) and migration (p<0.05) of the capillary endothelial cells compared with P29 and T42. These results suggest that decreased expression of 37LBP in T11 may relate to its low tumorigenicity, and that this may be partly caused by diminished tumor angiogenesis of the murine lung cancer.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Abe, K・Sato, k et.al.: "Presence of atypical laminin on the surface of mouse Lewis lung careinoma cells" Tohoku J Exp Med. 180. 33-44 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 田中 昌史・佐藤 研 他: "ラミニン結合蛋白37LBP/p40の発現抑制は腫瘍血管新生を抑制する" Connective Tissue(日本結合組織学会誌). 28. 237-245 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hara, k・Satoh, K.et.al.: "Apical ectodermal ridge-dependent expression of the chick 67kDa laminin binding protein gene in developing limb bud." Zool Science. 14. 967-978 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Abe K,Murakami S,Mukae N,Mita T,Hashimoto Y,Isemura M,Shimo-oka M,Ii K,Kitamura K,Narumi K,Satoh K,Nukiwa T.: "Presence of atypical laminin on the surface of mouse Lewis lung carcinoma cells." Tohoku J Exp Med. 180. 33-44 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakai T,Satoh K,Matsushima K,Shindo S,Abe S,Abe T,Motomiya M,Kawamoto T,Kawabata Y,Nakamura T,Nukiwa T: "Hepatocyte growth factor in bronchoalveolar lavage fluids and cells in patients with inflammatory chest diseases of the lower respiratory tract : Detection by RIA and in situ hybridization." Am J Respir Cell Mol Biol. 16. 388-397 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Abe T,Kikuchi T,Tominaga Y,Kikuchi T,Watanabe A,Satoh K,Watanabe Y,Nukiwa T.: "Bacterial pneumonia causes augmented expression of the secretory leukoprotease inhibitor gene in the murine lung." Am J Respir Crit Care Med.156. 1235-1240 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hara K,Satoh K,Ide H.: "Atypical ectodermal ridge dependent expression of the 67kDa laminin binding protein (clbp) gene in developing chick limb bud." Zool Science. 14. 969-978 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uchiyama B,Saijo Y,Kumano N,Abe T,Fujimura S,Ohkuda K,Handa M,Satoh K,Nukiwa T.: "Expression of nucleolar protein p120 in human lung cancer : difference in histological types as a maker for proliferation." Clinical Cancer Research. 3. 1873-1877 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maemondo M,Saijo Y,Ebina M,Abe T,Yaekashiwa M,Satoh K,Nukiwa T,Rapidly: "progressive primary adenocarcinoma of the lung with signet-ring cells responding to 5-fluorouracil and leucovorin : a case report" Int J Clin Oncol. 2. 47-50 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kikuchi T,Abe T,Satoh K,Narumi K,Sakai T,Abe S,Shindoh S,Matsushima K,Nukiwa T.: "Cis-acting region associated with lung cell-specific expression of the secretory leukoprotease inhibitor gene." Am J Respir Cell Mol Biol. 17. 361-367 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saijo Y,Uchiyama B,Abe T,Satoh K,Nukiwa T.: "Contiguous four guanosine sequence in c-myc antisense phosphorothioate oligonucleotide inhibits cell growth on human lung cancer cells : Possible involvement of cell adhesion inhibition." Jap J Cancer Res.88. 26-33 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yaekashiwa M,Nakyama S,Ohnuma K,Sakai T,Abe T,Satoh K,Matsumoto K,Nakamura T,Takahashi T,Nakiwa T.: "Simultaneous or delayd administration of hepatocyte growth factor (HGF) equally represses the fibrotic changes in murine lung injury induced by bleomycine : a morphologic study." Am J Respir Crit Care Mad.156. 1937-1944 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka M,Narumi K,Isemura M,Abe M,Sato Y,Saijo Y,Nukiwa T,Satoh K.: "Depressed expression of the 37-kDa laminin binding protein (37LBP) affects angiogenesis in murine lung tumor." Angiogenesis Res. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satoh K,Narumi K,Abe T,Sakai T,Kikuchi t, Tanaka M,ShimoOka T,Uchida M,Tezuka F,Isemura M,Nukiwa T.: "Diminution of 37-kDa laminin binding protein expression reduces tumor formation of murine lung cancer cells." Br J Cancer. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hara K, SAtoh K, et al.: "Apical Ectodermal Ridge-Depundent Expression of the chick 67 KDa Laminin Binding Pnoteui geue in Devecloping Limb Bud." Zool Science. 14. 969-978 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 田中 昌史: "ラミニン結合蛋白37LBP/p40の発現抑制は腫瘍血管新生に影響する" 日本結合組織学会雑誌(Corbnetive Tissue). 28. 237-245 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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