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Basic Reseach for the Establishment of Gene Immunotherapy Against Advanced Neuroblastoma

Research Project

Project/Area Number 08457229
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

MATSUMURA Takafumi  Kyoto Pref.Univ.of Medicine, Pediatrics, Assistant Professor, 医学部, 講師 (40219481)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥3,300,000 (Direct Cost: ¥3,300,000)
Keywordsneuroblastoma / gene immunotherapy / allogeneic MHC gene / MHC抗原
Research Abstract

Introduction :
Neuroblastoma is considered as an immunogenic tumor, because of the clinical evidence of spontaneous regression, presence of TAAs and more lymphocytic infiltration in regressing tumors. Therefore, the modification of tumor immunogenicity by transfer with allogeneic MHC gene could enhanced the host anti-tumor immunity.
Methods & Results :
1)S3 is a highly tumorigenic subclone of a C-1300 NB cell line from A/J mouse (H-2Kk). S3neo4 was a control line transfected with neor, while S3Kb4C3 was a transfectant with both nCMVintKb (carrying H-2Kb) and neor.
2)A/J mice were subcutaneously inoculated with tumor cells. Tumor rejection was observed in 5/20 miceinoculated with S3Kb4C3, but in none of mice inoculated with control cells. THe survival improvement was observed in mice inoculated with S3Kb4C3 compared to control mice. All mice survived rejected parent S3.
3)In 51Cr release assay, splenocytes from mice immunized with S3Kb4C3 effectively lysed S3Kb4C3 as well as S3, but not S1509a from A/J mouse.
4)Mice were immunized by intraperitoneal inoculation with INF-g-and MMC-treated control cells or S3Kb4C3, and were injected with HVJ-liposome into the established S3 tumors. Tumor regression was observed in 5/10 mice injected with HVJ-liposome containingn CMVintKb (H-2Kb protein expression in tumor cells injected with nCMVintKb was comfirmed by FACS), but in none injected with HVJ-liposome containing control DNA.
Conclusion :
Transfer with allogeneic MHC gene into tumor cells invitro as well as in vivo could be an effective gene immunotherapy and feasible for clinical application for neuroblastoma.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 石田 宏之、松村 隆文 ら: "アロ主要組織適合抗原(MHC)の遺伝子納入によるマウス神経芽腫(NB)に対する抗腫瘍免疫応答の増強" 小児がん. 32. 356- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 石田 宏之、松村 隆文 ら: "マウス神経芽腫に対する免疫遺伝子治療I.アロクラスIMHC遺伝子移入による効果" 日本癌学会総会記事. 55. 222- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishida H, Matsumura T et al: "Gene immanotherapy for murinx neunoblostoma(I)Transpeetion of an allogeneic MHC class I gene" Proceadings of the 2nd Annual Meating of the Japan Society of Geue. 2. 94- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishida H, Matsumura T et al: "MAGE-1 and MAGE-3/6 expreoyon in neuroblostoma-related pedcatric solid tumors" Int.J.Cancer. 69. 375-380 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 山根 秀一, 松村 隆文 ら: "ヒト神経芽腫に対する免疫遺伝子治療I.allo-MHC class I遺伝子納入の効果" 日本癌学会総会記事. 56. 709- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishida H, Matsumura T et al: "Effect of allegeneic MHC gene transfer by HVJ-liposome against murone nebroblastome" Proceedings of the American Association for Canar Research. 38. 13- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishida H,Matsumura T,Salgaller ML,Ohmizono Y,Kadono Y and Sawada T.: "MAGE-1 and MAGE-3/6 expression in neuroblastoma-related pediatric solid tumors." Intl J Cancer. 69. 375-380 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishida H,Matsumura T,Plautz G,Yamane Y,Sawada T and Kaneda Y.: "Effect of allogeneic MHC gene transfer by HVJ-liposome against murine neuroblastoma." Proceedings of American Association for Cancer Research. 38. 13 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamane S,Matsumura T,Ishida H,Boon T,Lurquin C.: "Gene immunotherapy for human neuroblastoma : (I) Ex vivo transfection with allo-MHC class I and beta2-microglobulin genes." Proceedings of the 3rd Annual Meeting of the Japan Society of Gene Therapy.(1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumura T,Ishida H,Salgaller ML,Sawada T.: "MAGE-1 and MAGE-3 expression in human neuroblastomas." Proceedings of the 7th International Symposium on Advances in Neuroblaston Research. A24. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishida H,Matsumura T,Yamane S,Sawada T and Kaneda Y.: "Gene immunotherapy for murine neuroblastoma : (I) Transfection of an allogeneic MHC class gene." Proceedings of the 2nd Annual Meeting of the Japan Society of Gene Therapy.94 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishida H,Matsumura T,Sawada T,Plautz G.Immune: "response enhancement of allo-MHC induced murine neuroblastoma (NB) : A preliminary study on gene therapy for NB." Proceedings of International Symposium on Human Gene Therapy.3 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumura, T., Ishida, H., Kadono, Y., Ohmizono, Y., Hosoi, H., Sawada, T.and Salgaller, M.L.: "Gene expression of the MAGE-1-encoding human melanoma antigen in pediatric tumors." Proceedings of American Association for Cancer Research. 35. 497 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 石田宏之、 松村隆文ら: "アロ主用組織適合抗原(MHC)の遺伝子移入によるマウス神経芽腫(NB)に対する抗腫瘍免疫応答の増強" 小児がん. 32. 356 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] 石田宏之、 松村隆文ら: "マウス神経芽腫に対する免疫遺伝子治療I.アロクラスIMHC遺伝子移入による効果" 日本癌学会総会記事. 55. 222 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishida H, Matsumura T.et al.: "Gene immanotherapy for murine neurohkstoma(I) Trans Peition of on allogenais MHC clss I gene" Proccadings of the 2nd Annual Meating of the Japan Society of Gene. 2. 94 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishida H, Matsumura T.et al: "MAGE-1 and MAGE-3/6 expredyon in neuroblostoma-related pekeatric solid tumers" Int.J.Cancer. 69. 375-380 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山根秀一, 松村隆文ら: "ヒト神経芽腫に対する免疫遺伝子治療I.allo-MHC class I遺伝子移入の効果" 日本癌学会総会記事. 56. 709 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishida H, Matsumura T.et al: "Effect of allegeneic MHC gene transfer by HVJ-1iposome against murine nelroblastoma" Proceedings of the American Association for Cancer Research. 38. 13 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishida, H: "Enhancement of immune respones against murire neuroblastoma by tronsfection with anallo-MHC Class I Ag" Proceedings of Advances in Neuroblastoma Reserch. C18 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石田宏之: "マウス神経芽腫に対する免疫遺伝子治療 I.アロクラスI遺伝子移入による効果" 日本癌学会総会記事. 222 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishida, H: "Immune response enhancement of anallo-MHC induced induced mucrine neuroblastoma : Preliminary study on gene therapy for geuroblatoma" Proceedings of International Symposium on Heman Gene Thelapy. (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石田宏之: "アロ主要組織適合抗原(MHC)の遺伝子移入によるマウス神経芽種に対する抗腫瘍免疫応答の増強" 小児がん. 32・3. 356 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishida, H: "Gene immanotherapy for murine neuroblastoma : I. Transfection of an allogeneic NHC Class I Gene" Proceedings of the Second Annual Meeting of the Japan Society of Gene Therapy. 2. 94 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsumura. T: "Prospective analysis and cicrrentstra tegy for management of infantile neuroblastoma in Japan" The Korean Journal of Pediatric Hematology Oncology. 3・2. 379-381 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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