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Role of the juxtaglomerular apparatus in hypertension and progressive renal impairment

Research Project

Project/Area Number 08457283
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionTohoku University

Principal Investigator

ITO Sadayoshi  Tohoku Univ.2nd Dept.Int.Med.Prof., 医学部, 教授 (40271613)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Kazuo  Tsukuba Univ.Biochem.Prof., 応用生物化学系, 教授 (70110517)
ABE Keishi  Tohoku Univ.Merit Prof., 医学部, 名誉教授 (60004777)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 1997: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1996: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsafferent and efferent arterioles / microperfusion / angiotensin II / receptors / P450 / arachidonic acid / Heterogeneity / renal hemodynamics / 糸球体輸入細動脈 / 微少灌流 / アンジオテンシン II / 皮質ネフロン / 棒随質ネフロン / 間質 / アンギオテンシンII / AT_1受容体 / AT_2受容体 / 内皮 / 一酸化窒素 / プロスタグランディン / 輸出入細動脈
Research Abstract

Glomerular and tubular functions are important for homeostasis of body fluid and electrolytes, and hence for the regulation of systemic blood pressure. In this project we employed an in vitro preparation in which microdissected afferent (Af-Arts) or efferent arterioles (Ef-Arts) are perfused at a controlled pressure.
Althought angiotensin I.I type 2 receptor (AT_2R) has recently been cloned, its functional role is not well understood. We found that selective activation of AT_2R causes vasodilation in the both the Af-Art and Ef-Art. The degree of vasodilation was simular in the two arterioles. In the Af-Art the dilation was unaffected by inhibiting either nitric oxide synthase or cycloxygenase, however, it was abolished by either disrupting the endothelium or inhibiting the cytochrome P-450 pathway, particularly the synthesis of epoxyeicosatrienoic acids (EETs). These results suggest that in the Af-Art activation of the AT_2 receptor may cause endothelium-dependent vasodilation via a cytochrome P-450pathway, possibly by EETs. We also studied heterogeneity of Ang II action within the kidney. We found that intraluminal administration of Ang II caused stronger vasoconstriction than extraluminal administration only in the superficial but not juxtamedullary Af-Arts. In addition, Ang II-induced vasoconstriction was stronger in the superficial than juxtamedullary Af-Arts. Such heterogeneities may be important in order for the kidney to maintain homeostasis of body fluid and electrolytes.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] Arima, S・Y, Ren・J, A, Juncos and S, Ito: "Platelet-activating factor dilates efferent arterioles via glomerulus-derived nitric oxide." J.Am.Soci.Nephrol.7. 90-96 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J, A Jucos・Arima, S・S, Ito et al.: "Angiotensin II action in isolated microperfused rabbit afferect arterioles is modulated by flow." Kidney Int.49. 374-381 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y, Ren・O, A, Carretero・S, Ito.: "Influence of NaCl concentration at the macula densa on angiotensin II-induced constriction of the afferent arteriole." Hypertension.27. 649-652 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J, Amin・O, A, Carretero・S, Ito.: "Mechanisms of action atrial natriuretic factor and C-type natrinretic peptide." Hypertension.27. 684-687 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arima, S・K, Omata・S, Ito・K, Abe., et al.: "20-HETE requires increased vasoular tone to costrict rabbit afferent arterioles." Hypertension.27. 781-785 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito, S・K, Abe.: "Influence of anti-hypertensive drugs on glamerular hemodynamics." Jpn.J.Nephrol.38. 115-118 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito, S and K, Abe.: "Tubuloglomerular feedback" Jpn.Heart.J.37. 153-163 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito, S・O, A, Carretero・K, Abe.: "Nitric oxide in the juxtaglomerular apparatus." Kindney Int.49. S6-S8 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arima, S・S, Ito・K, Omata・K, Abe., et al.: "Diverse effects of calcium antagonists on the glomerular hemodynamics." Kindney Int.49. S132-S134 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Watanabe, N・K, Tsunoda・S, Ito・K, Abe., et al.: "Bilateral aldosterone-producing adenomas in two patients diagnosed by immunchistochemical analysis of steroidogenic enzymes." Tohoku J.Exp.Med.179. 123-129 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Abe, K・S, Ito.: "The Kidney and hypertension." Hyperts Res.20. 75-84 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito, S: "The kidney and hypertension:role of the juxtaglomerular apparatus." Tohoku J.Exp.Med.181. 411-429 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito, S・K, Abe.: "Contractile properties of afferent and efferent arterioles." Clin.Exp.Pharmacol.Physiol.24. 532-535 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tto, S・O, A, Carretero・K, Abe.: "Role of nitric oxide in the control of glomerular circulation." Clin.Exp.Pharmacol.Physiol.24. 578-581 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsutsumi, E・K, Takeuchi・S, Ito・K, Abe., et al.: "Rat kidney thromboxane synthase:cDNA cloning and gene expression regulation in hydronechrotic kidney." Prostaglandins.53. 423-431 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Endo, Y・A, Arima・K, Abe・S, Ito., et al.: "Functions of angiotensin II type2 receptor in the postglomerular efferent arteriole." Kidney Int.52. S205-S207 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito, S・J, Amin・Y, L, Ren・S, Arima・K, Abe., et al.: "Heterogeneity of angiotensin action in renal circulation." Kidney Int.52. S128-S131 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arima, S・Y, Endo・K, Abe・S, Ito., et al: "Possible role P450 metabolate of arachidonic acid in vascular mechanism of angiotensin II type2 receptor in the isolated microperfused rabbit afferent arteriole." J.Clin.Invest.100. 2816-2823 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi, K・T, Kato・S, Ito・k, Abe., et al.: "Three cases of Gitelman's syndrome possibly caused by different mutations in the thiazide-sensitive Na-Cl cotransporter." Intern.Med.36. 532-585 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arima S., et al.: "Platelet-activating factor dilates efferent arterioles via glomerulus-derived nitric oxide." J.Am.Soci.Nephrol.7. 90-96 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Juncos IA., et al.: "Angiotensin II action in isolated microperfused rabbit afferent arterioles is modulated by flow." Kidney Int.49. 374-381 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ren Y., et al.: "Influence of NaCl concentration at the macula densa on angiotensin II-induced constriction of the afferent arteriole." Hypertension. 27. 649-652 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Amin J., et al.: "Mechanisms of action atrial natriuretic factor and C-type natriuretic peptide." Hypertension. 27. 684-687 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arima S., et al.: "20-HEIE requires increased vascular tone to constrict rabbit afferent arterioles." Hypertension. 27. 781-785 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito S., et al.: "Influence of anti-hypertensive drugs on glomerular hemodynamics." Jpn.J.Nephrol.38. 115-118 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito S., et al.: "Tubuloglomerular feedback." Jpn.Heart.J.37. 153-163 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito S., et al.: "Nitric oxide in the juxtaglomerular apparatus." Kidney Int.49 (Suppl.55). S-6-S-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arima S., et al.: "Diverse effects of calcium antagonists on the glomerular hemodynamics" Kidney Int.49 (Suppl.55). S-132-S-134 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Watanabe N., et al.: "Bilateral aldosterone-producing adenomas in two patients diagnosed by immunchistochemical analysis of steroidogenic enzymes." Tohoku J.Exp.Med.179. 123-129 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Abe k., et al.: "The kidney and hypertension" Hyperts.Res.20. 75-84 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito S.: "The kidney and hypertension : role of the juxtaglomerular apparatus" Tohoku J.Exp.Med.181. 411-429 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito S., et al.: "Contractile properties of afferent and efferent arterioles." Clin.Exp.Pharmacol.Physiol.24. 532-535 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito S., et al.: "Role of nitric oxide in the control of glomerular circulation" Clin.Exp.Pharmacol.Physiol.24. 578-581 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsutsumi E., et al.: "Rat kidney thromboxane synthase : cDNA cloning and gene expression regulation in hydronephrotic kidney" Prostaglandins.53. 423-431 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Endo Y., et al.: "Functions of angiotensin II type 2 receptor in the postglomerular efferent arteriole" Kidney Int.52 (Suppl.63). S205-S207 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito S., et al.: "Heterogeneity of angiotensin action in renal circulation" Kidney Int.52 (Suppl.63). S128-S131 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arima S., et al.: "Possible role P450 metabolate of arachidonic acid in vasculator mechanism of angiotensin II type 2 receptor in the isolated microperfused rabbit afferent arteriole." J.Clin.Invest.100. 2816-2823 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi K., et al.: "Three cases of Gitelman's syndrome possibly caused by different mutations in the thiazide-sensitive Na-Cl cotransporter." Intern.Med.36. 532-585 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Abe, K., Ito, S.: "The Kidney and Hypertension" Hypertens.Res.20. 75-84 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ito, S.: "The Kidney and hypertension : Role of the juxtag lomerular apparatus" Tohoku J.Exp.Med.181. 411-429 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ito, S., Abe, K.: "Contractile properties of afferent and efferent arteriole" Clin.Exp.Pharmacol.Physiol.24. 532-535 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ito, S., Carretero, O.A., Abe, K.: "Role of nitric oxide in the control of glomerular circualtion" Clin.Exp.Pharmacol.Physiol.24. 578-581 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsutsumi, E., Takeuchi, K., Ito, S., et al.: "Rat kidney thromboxane syntase : cDNA cloning and gene expression regulation in hydronephrotic kidney" Prostaglandins. 53. 423-431 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Endo, Y., Arima, S., Ito, S., et al.: "Functions of angiotensin II type-2 receptor in the postglomerualr efferent arteriole" Kidney Int.52 (Suppl.63). S205-S207 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ito, S., Amin, Y.L., Ren, Y.L., et al.: "Heterogeneity of angiotensin action in renal circualtion" Kidney Int.52 (Suppl.63). S128-S131 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Arima, S., Endo, Y., Ito, S., et al.: "Possible role P450 metabolate of arachidonic acid in vasculator mechanism of angiotensin II type 2 receptor in the isolated microperrused rabbit Af-Art" J.Clin.Invest.100. 2816-2823 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeuchi, K., Kato, T., Ito, S., et al.: "Three cases of Gitelman's.syndrome possibly caused by different mutations in the thiazide-sensitive Na-C1 cotransporter" Intern.Med.36. 562-585 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] ITO S, Carretero OA, Abe K: "Nitic oxide in the regulation of renal blood flow." New Horizon. 3. 615-623 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Arima S, Ren Y, Juncos LA, ITO S: "Platelet-activating factor dilates efferent arterioles via glomerulus-derived nitric oxide." J. Am. Soci. Nephrol.7. 90-96 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Juncos LA, Arima S, Garvin J, ITO S, et al.: "Angiotensin II action in isolated microperfused rabbit afferent arterioles is modulated by flow." Kidney Int.49. 374-381 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ren Y, Carretero OA, Ito S: "Effect of NaCl concentration at the macula densa on angiotensin II-induced constriction of the afferent arteriole." Hypertension. 27. 649-652 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Amin J, Carretero OA, Ito S: "Differential mechanism of action of atrial natriuretic factor and C-type natriuretic peptide in the juxtamedullary nephron." Hypertension. 27. 684-687 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Arima S, Omata K, Ito S, Abe K, et al.: "Increase in the vascular tone triggess vasoconstrictor action 20-HETE in the afferent arteriole." Hypertension. 27. 781-785 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito S, Abe K: "Influence of anti-hypertensive drugs on glomerular hemodynamics." Jpn. J Nephrol.38. 115-118 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito S, Abe K: "Tubuloglomerular feedback" Jpn. Heart J.37. 153-163 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito S, Carretero OA, Abe K: "Nitric oxide in the juxtaglomerular apparatus." Kidney Int.49(Suppl). S6-S8 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Arima S, Ito S, Omata K, Abe K, et al.: "Diverse effects of calcium antagonists on the glomerualr hemodynamics." Kidney Int.49 (Suppl). S132-S134 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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