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Role of 3-deoxyglucosone in the pathogenesis of uremic and diabetic complications

Research Project

Project/Area Number 08457287
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionNagoya University

Principal Investigator

NIWA Toshimitsu  Nagoya University, School of Medicine Associate Professor, 医学部, 助教授 (20208268)

Co-Investigator(Kenkyū-buntansha) NAGAYA Takashi  Nagoya University, Research Institute of Enveronmental Medicine Research Associa, 環境医学研究所, 助手 (80262913)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords3-deoxyglucosone / imidazolone / uremia / diabetes mellitus / amyloid / AGE / カルボキシメチルリシン / 糖尿病性腎症 / 透析アミロイドーシス / 動脈硬化 / メイラード反応
Research Abstract

3-Deoxyglucosone (3-DG) is a reactive intermediate of the Maillard reaction which is a nonenzymatic glycation reaction of proteins. To investigate the role of 3-DG in the pathogenesis of uremic and diabetic complications, we produced and anti-AGE antibody (AG-1) that reacted specifically with imidazolone, a novel advanced glycation end products (AGE). The imidazolone is the reaction products of the guanidino group of arginine with 3-DG.We demonstrated that amyloid tissue beta_2m is modified with imidazolone in patients with DRA.Further, we studied the localization of imidazolone in the kidneys and aortas obtained from diabetic patients by immunohistochemistry using the antibody. Specific imidazolone immunoreactivity was detected in nodular lesions and expanded mesangial matrix of glomeruli, and renal arteries in advanced stage of diabetic nephropathy as well as in atherosclerotic lesions of aortas. These results, taken together with a recent demonstration of increased serum 3-DG levels in uremia and diabetes, strongly suggest that imidazolone produced by 3-DG may contribute to the progression of long-term uremic and diabetic complications such as dialysis-related amyloidosis, and diabetic nephropathy and atherosclerosis.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 丹羽 利充: "Amyloid β2-microglobulin is modified with imidazolone,a novel advanced glycation end product,in dialysis-related amyloidosis." Kidney Int. 51. 187-194 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 丹羽 利充: "Immunohistochemical detection of imidazolone,a novel advanced glycation end product,it kidneys and aortas of diabetic patients." J Clin Invest. 99. 1272-1280 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 丹羽 利充: "Imidazolone,a novel advanced glycation end product,is present at high levels in kidneys of rats with streptozotocin-induced diabetes." FEBS Lett. 407. 297-302 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 丹羽 利充: "β2-Microglobulin dialysis amyloid and its formation : role of 3-deoxyglucosone and advanced glycation end products." Nephron. 76. 373-391 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Niwa T,Katsuzaki T,Miyazaki S,Momoi T,Akiba T,Miyazaki T,Nokura K,Hayase F,Tatemichi N,Takei Y.: "Amyloid beta2-microglobulin is modified with imidazolone, a novel advanced glycation end product, in dialysis-related amyloidosis." Kidney Int. 51. 187-194 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Niwa T,Katsuzaki T,Miyazaki S,Miyazaki T,Ishizaki Y,Hayase F,Tatemichi N,Takei Y.: "Immunohistochemical detection of imidazolone, a novel advanced glycation end product, in kidneys and aortas of diabetic patients." J Clin Invest. 99. 1272-1280 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Niwa T,Katsuzaki T,Ishizaki Y,Hayase F,Miyazaki T,Uematsu T,Tatemichi N,Takei Y.: "Imidazolone, a novel advanced glycation end product, is present at high lovels in kidneys of rats with streptozotocin-induced diabetes." FEBS Lett. 407. 297-302 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Niwa T.: "beta2-Microglobulin dialysis amyloid and its formation : role of 3-deoxyglucosone and advanced glycation end products." Nephron. 76. 373-391 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 丹波利充: "Amyloid β2-microglobulin is modified with imidazolone,a novel advanced glycation end product,in dialysis-related amyloidosis." Kidney Int. 51. 187-194 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 丹羽利充: "Immunohistochemical detection of imidazolone,a novel advanced glycation end product,in kidneys and aortas of diabetic patients." J Clin Invest. 99. 1272-1280 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 丹羽利充: "Imidazolone,a novel advanced glycation end product,is present at high levvels in kidneys of rats with streptozotocin-induced diabetes." FEBS Lett. 407. 297-302 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 丹羽利充: "β2-Microglobulin dialysis amyloid and its formation: role of 3-deoxyglucosone and advanced glycation end products." Nephron. 76. 373-391 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Niwa T,et al.: "Amyloid β2-microglobulin is modified with N'-(carboxymethyl) lysine in dialysis-related amyloidosis." Kidney Int. 50. 1303-1309 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Niwa T,et al.: "Amyloid β2-microglobulin is modified with imidazolone,a novel advanced glycation end product,in dialysis-related amyloidosis." Kidney Int. 51. 187-194 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Niwa T,et al.: "Immunohistochemical detection of imidazolone,a novel advanced glycation end product,in kidneys and aortas of diabetic patients." J Clin Invest. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Niwa T: "β2-Microglobulin dialysis amyloid and its formation : role of 3-deoxyglucosone and advanced glycation end products." Nephron. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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