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Development of a new HLA-DNA typing method applicable in the nation-wide renal transplantation network.

Research Project

Project/Area Number 08457313
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionSaitama Medical School

Principal Investigator

MAEDA Hiroo  Saitama Medical School, Faculty of Medicine, Professor, 医学部, 教授 (30134597)

Co-Investigator(Kenkyū-buntansha) SUWATA Junji  Saitama Medical School, Faculty of Medicine, Assistant, 医学部, 助手 (30150618)
上甲 覚  埼玉医科大学, 医学部, 助手 (80246470)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsKidney transplantation / HLA / DNA typing / HPA
Research Abstract

HLA typing of both donor and recipients in organ transplantation is important from the stand point of equity and efficiency in unrelated cadaver organ transplantation. We have already developed a new HLA-DNA typing method using a RNA amplification method targeting at the genomic DNA,Transcription-Mediated Amplification (TMA) and a chemiluminescence-based Hybridization Protection Assay (HPA), which is one type of sequence-specific oligonucleotide (SSO) methods. In this research, we combined polymerase chain reaction (PCR) with HPA method, to analyze further fine alleles of the DR6 antigen group. One-hundred and twenty-one DNA samples were amplified with DR356-specific primers by PCR and detected by HPA method. Among DRB1^<**>13 allele group, 10 alleles (DRB1^<**>130l-1307, ^<**>1310-1312) were derfinitely typed, and among DRB1^<**>14,11 a11eles (DRBl^<**>l401-1408, ^<**>1411^<, **>1412, ^<**>l417). However, a certain combination of alleles (^<**>1301/02, ^<**>1140l/07) were not typed unequivocally and need additional primer combinations. The procedure time required for PCR-HPA method is one hour for extraction of DNA,2 hours for amplification, and one hour for detection, a total of 4 hours. Thus, PCR-HPA method for HLA-DNA typing is a simple, fast, non-isotopic and accurate method, which will guarantee the equity and efficiency in organ transplantation.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 平田 蘭子: "TMA-HPA法によるHLA-DRB1タイピングキットの評価" 臨床検査. 40(2). 241-247 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 沼賀 二郎: "強直性脊椎炎にみられる ぶどう膜炎とHLA" 日眼会誌. 100. 292-295 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suwata, J: "HLA class II DNA genetyping by TMA-HPA method" Human lmmunal. 47. 46-46 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi, A: "A new sensitive and simple method for HLA calss II DNA" Vox Sang. 70(S2). 132-132 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda, H: "AHSH15:HLA-DR6,DR1403,DR1404,DR52" Genetic diversity of HLA. 1. 107-119 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saitoh, M: "Polymorphisms of transporter associated with antigen" Genetic diversity of HLA. 2. 254-256 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirata R,et al.: "Evaluation of HLA-DRBl typing kit by TMA-HPA method" Rinsho Kensa. 40(2). 241-247 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Numaga J.et al.: "AAU in ankylosing spondylitis and HLA" Jap J Ophthalmo1. 100(4). 292-295 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi A,et al.: "A new sensitive and simple method for HLA class II DNA typing : Transcription mediated amplification-hybridization protection assay (TMA-HPA)." Vox Sang. 70(S2). 132 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suwata J,et al.: "HLA class II DNA genotyping by TMA-HPA method for kidney transplantation." Human Immunol. 47(1,2). 46 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda H,et al.: "AHS#15 ; HLA-DR6, DRl403, DR1404, DR52." In Genetic diversity of HLA.vol 1 107-119, EDK,Paris. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saitoh M,et al.: "Polymorphisms of transporter associated with antigen processing (TAP) genes in recurrent spontaneous abortion." In Genetic diversity of HLA.vol 2 254-256, EDK,Paris. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Joko S,et al.: "HLA and leprosy in Japanese." In Genetic diversity of HLA.vol 2 693-694, EDK,Paris. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 前田平生: "AHS#15 ; HLA-Dr6,Dr1403,Dr1404,Dr52" Genetic divevsity of HLA. vol.1. 107-119 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Saitoh,M.: "Polymorphisms of TAP qenes in vecutrent spontaneous abortion." Genetic divevsity of HLA. vol.2. 254-256 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Joko,S.: "HLA and leprosy in Japanese" Genetic diversity of HLA. vol.2. 693-694 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小林 腎: "第6回日本組織適合性学会QCワークショップ報告" MHC. 4(2). 100-109 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 平田蘭子: "HLA検査・DNA検査" 検査と技術. 25(7). 141-146 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 前田平生: "TMA法" 検査と技術. 25(7). 194-194 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 斉藤正博: "反復流産症例における免疫療法と抗HLA抗体産生" 日本不妊学会雑誌. 42. 83-89 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 上甲覚: "らい(ハンセン病)の上強膜炎とヒト主要組織適合抗原" 日本眼科学会雑誌. 101. 167-172 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 平田蘭子: "TMA法によるHLA-DRの検査" 検査と技術. 24. 1151-1153 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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