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Molecular detection of cancer cells by RT-PCR

Research Project

Project/Area Number 08457326
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MORI Masaki  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,ASSOCIATE PROFESSOR, 生体防御医学研究所, 助教授 (70190999)

Co-Investigator(Kenkyū-buntansha) OTSUKA Makoto  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,ASSOCIATE PROFESSOR, 生体防御医学研究所, 助教授 (60203840)
YOSHIKAWA Yasuji  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,ASSOCIATE PROFESSOR, 生体防御医学研究所, 助教授 (80124816)
YAMAGATA Motoyuki  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,LECTURER, 生体防御医学研究所, 助手 (90294975)
HARAGUCHI Masaru  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,ASSISTANT PROFESSOR, 生体防御医学研究所, 講師 (40228531)
三森 功士  九州大学, 生体防御医学研究所, 医員
楠本 宏記  九州大学, 生体防御医学研究所, 助手 (00195447)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 1997: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1996: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsgastrointestinal cancer / breast cancer / genetic diagnosis / minute cancer cells / molecular biology / RT-PCR / recurrence / prognosis
Research Abstract

1.We analyzed the mRNA expression of carcinoembryonic antigen (CEA), estrogen receptor (ER) and MAGE in tumor and normal epithelial tissues by means of PT-PCR.CEA was detectable in almost all tumor and normal epithelial tissues. ER was detectable in 1/3 of the samples. MAGE was not expressed in normal epithelial tissues, but was expressed in 10-60% tumor tissues. The results indicated that the best target gene was CEA.
2.The positive detection rate increased from 20% by histologic examination to 60% by RT-PCR.The recurrence rate was 35% in 37 cases showing positive results in both examinations, 8% in 47 cases showing histologically negative but RT-PCR positive results, and none in 18 cases showing negative results in both examinations.
3.The positive detection rate for CEA mRNA in peripheral blood samples increased with advancing stage of disease. With respect to 62 curatively operated cases, CEA mRNA was detected in 12 cases. Four of these 12 cases developed metastatic disease after surgery whereas none of 50 cases negative by RT-PCR developed metastasis.
4.It has been shown that RT-PCR is a powerful tool to detect CEA mRNA in the lymph nodes or the peripheral blood. This is potentially very useful to determine high-risk patients for metastasis.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Mori, M.: "Clinical significance of molecular detection of carcinoma cells in iymph nodes and peripheral blood by reverse transcription-polymerase…" J Clin Oncol. 16. 128-132 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, M.: "p27 expression and gastric carcinoma." Nature Med. 3. 593 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, M.: "Analysis of MT1-MMP and MMP2 expression in human gastric cancers." Int J Cancer. 74. 316-321 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka, F.: "Higher frequency of the expression of the MAGE gene family in human esophageal carcinoma." Int J Oncol. 10. 1113-1117 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujie, T.: "Expression of MAGE and BAGE genes in Japanese breast cancers." Ann Oncol. 8. 369-372 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, M.: "Molecular Diagnosis of Circulating Cancer Cells Using MAGE Gene Assays." JAMA. 278. 476-477 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, M: "Clinical significance of molecular of carcinoma cells in iymph nodes and peripheral blood by reverse transcriptionpolymerase・・・" J Clin Oncol. 16. 128-132 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, M.: "p27 expression and gastric carcinoma." Natur Med. 3. 593 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, M.: "Analysis of MT1-MMP and MMP2 expression in human gastric cancers." Int J Cancer. 74. 316-321 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka, F.: "Higher frequency of the expression of the MAGE gene family in human esophageal carcinoma." Int J Cancer. 10. 1113-1117 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujie, T.: "Expression of MAGE and BAGE genes in Japanese breast cancers." Ann Oncol. 8. 369-372 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, M.: "Molecular Diagnosis of Circulating Cancer Cells Using MAGE Gene Assays." JAMA. 278. 476-477 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori,M.: "Clinical significance of molecular detection of carcinoma cells in iymph nodes and peripheral blood by reverse transcription-polymerase…" J Clin Oncol. 16. 128-132 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,M.: "p27 expression and gastric carcinoma." Nature Med. 3. 593-593 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,M.: "Analysis of MT1-MMP and MMP2 expression in human gastric cancers." Int J Cancer. 74. 316-321 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka,F.: "Higher frequency of the expression of the MAGE gene family in human esophageal carcinoma." Int J Oncol. 10. 1113-1117 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujie,T.: "Expression of MAGE and BAGE genes in Japanese breast cancers." Ann Oncol. 8. 369-372 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,M.: "Molecular Diagnosis of Circulating Cancer Cells Using MAGE Gene Assays." JAMA. 278. 476-477 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,M.: "The molecular detection of circulating solid carcinoma cells in the peripheral blood:The concept of early systemic disease" Int J Cancer. 68. 739-743 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori,M.: "Expression of MAGE genes in human colorectal carcinoma" Ann Surg. 224. 183-188 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori,M.: "Detection of cancer micrometastases in lymph nodes by the reverse transcriptase-polymerase chain reaction" Cancer Res. 55. 3417-3420 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Adachi,Y.: "Prediction of early and recurrence after curative resection for gastric carcinoma" Cancer. 77. 2445-2448 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Honda,M.: "Martix metalloproteinase-7 expression in gastric carcinoma" Gut. 39. 444-448 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohta,M.: "The human FHIT gene,spanning the chromosome 3p14.2 fragile site and renal carcinoma associated translocation breakpoint,is abnormal in digestive tract cancers" Cell. 84. 587-597 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森 正樹: "分子生物学的にみた大腸癌の予後因子" 癌と化学療法. 23. 982-989 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森 正樹: "消化器癌における腫瘍拒絶抗原遺伝子の発現と臨床応用の可能性" 醫學のあゆみ. 178. 830-831 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森 正樹: "癌拒絶抗原" 外科. 58. 1820-1824 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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