Monecular aspect of cholesterol metabolism in gallstone formation
| Project/Area Number |
08457327
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | MIYAZAKI MEDICAL COLLEGE |
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Principal Investigator |
SETOGUCHI Toshiaki Miyazaki Medical College, Surgery I,Professor, 医学部, 教授 (40041420)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥6,200,000 (Direct Cost: ¥6,200,000)
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| Keywords | cholesterol 7alpha-hydroxylase / 25-hydroxyvitamin D_3 1alpha-hydroxylase / enzyme activity / substrate specificity / bile acids / vitamin D_3 |
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| Research Abstract |
1. Assay of human cholesterol 7alpha-hydroxylase
Microsomal cholesterol 7alpha-hydroxylase activity was not assayed as an increased measurement when we measured it after solubilizing the microsomes and reconstituting the P450 reaction system ideally ; whereas mitochondrial cholesterol 27-hydroxylase activity was assayed as a markedly increased measurement when we measured it after solubilizing the mitochondria and reconstituting the P450 reaction system ideally.
The results will be published.
2. Cholesterol and vitamin D_3 metabolisms
Cholesterol 27-hydroxylase and vitamin D_3 25-hydroxylase are performed by one common enzyme.
To survey these phenomena, I focused to study enzymes concerning vitamin D_3. First, we purified 25-hydroxyvitamin D_3 1alpha-hydroxylase from rat kidney mitochondria, and published as two papers.
Substrate specificity of the enzyme will be studied in future.
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Report
(3 results)
Research Products
(11 results)
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[Publications] Nakamura, Y., Eto, T.-A., Taniguchi, T., Miyamoto, K., Nagatomo, J., Shiotsuki, H., Sueta, H., Higashi, S., Okuda, K.-I.and Setoguchi, T.: "Purification and characterization of 25-hydroxyvitamin D_3 1alpha-hydroxylase from rat kidney mitochondria." FEBS Letters. 419(1). 45-48 (1997)
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[Publications] Eto, T.-A., Nakamura, Y., Taniguchi, T., Miyamoto, K., Nagatomo, J., Maeda, Y., Higashi, S., Okuda, K.-I.and Setoguchi, T.: "Assay of 25-hydroxyvitamin D_3 1alpha-hydroxylase in rat kidney mitochondria." Analytical Biochemistry. 258. 53-58 (1998)
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[Publications] Kimura, A., Kondo, K.-H., Okuda, K.-I., Higashi, S., Suzuki, M., Kurosawa, T., Tohma, M., Inoue, T., Nishiyori, A., Yoshino, M., Kato, H,and Setoguchi, T.: "Diagnosis of the first Japanese patient with 3-oxo-DELTA^4-steroid 5beta-reductase deficiency by use of immunoblot analysis." European Journal of Pediatrics. 157. 386-390 (1998)
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