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Development and clinical evaluation of a new lipid microsphere-encapsulated antifungal drug.

Research Project

Project/Area Number 08457331
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionWakayama Medical Scool

Principal Investigator

TANIMURA Hiroshi  Wakayama Medical School Professor, 医学部, 教授 (10026990)

Co-Investigator(Kenkyū-buntansha) TSUNODA Takuya  Wakayama Medical School Assistant, 医学部, 助手 (30275359)
UCHIYAMA Kazuhisa  Wakayama Medical School Lecturer, 医学部, 講師 (80232867)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsAmphotericin / lipid-Microsphere / Candida albicans / Abdominal fungal abscess / Candidemia / PCR / competitive PCR / fungal translocation / lipid-Microsphrer / C.albicans / A el fungal abscess / Candida / lipid-Microsphere / 深在性真菌症 / 脂肪乳剤 / 真菌症のPCR診断
Research Abstract

Incorporating AMPH into lipid emulsion(AMPH-lipid microsphere), wich if prepared by emulsification, was investigated for its structure and evaluated for its toxicity in vivo and in votro, and its efficacy in invasive general candidiasis in BALBc mice. The diameter of AMPH-lipid microsphere was small and stable during 90 days. It seemed that AMPH could be located in the phospholipid layer in the lipid complex formation. In vitro, AMPH-lipid rnicrosphere was less toxic aganisl hemolysis than the conventional AMPH(Fungizon). In vivo, AMPH-lipid microsphere was markedly less toxic in the survival rate of mice. It have shown an increased therapeutic index resulting in higher dose-dependent efficacy mice with systemic a new of model of local intraperitoneal candidasis. These findings suggested that incorporating AMIPH into lipid emulsion might be advantageous for treatment of candidiasis in surgery.
In the present study, a Candida specific PCR was performed to analyze by using the specific primer for V4 region of 18s gene of Candida species. To quantify the Candida, new heterogenous DNA competitor(comp V4 : 227bp) was newly made, and competitive PCR was periformed. Then it clarified that quantitiative measurement from PCR product was posible to analyze under the fg level of Candida-DNA.
It indicated that via portal vein was one pathway of fungal translocation, because Candida was detected by PCR from the portal venous blood during operatoin. Thus the Candida specific PCR may be an excellent tool for early diagnosis of deep mycosis clinically and for further understanding of fungal translocation.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] 谷村 弘: "新抗菌薬臨床評価の現状と問題点-新しいガイドラインの位置付け. 外科領域感染症" 日本化学療法学会雑誌. 45(9). 792-796 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 谷村 弘: "成分分析からみた肝内結石の成因" 胆道. 11(3). 237-248 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 内山和久: "日本人胆石の特徴と無症状胆石" 胆と膵. 19(4). 275-278 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 内山和久: "内視鏡的乳頭括約筋切開術" 消化器外科. 21(5). 798-801 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 角田卓也: "腫瘍免疫-最近の進歩ペプタイドと腫瘍免疫「臨床編」" 癌治療と宿主. 10(1). 99-104 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 角田卓也: "ペプタイドを用いる癌特異的免疫療法の飛躍的成績向上のための新戦略" Biotherapy. 12. 578 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 谷村 弘: "外科と栄養. 栄養・健康科学シリーズ, 臨床栄養学" 南江堂, 東京, 14 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 谷村 弘: "起炎菌からみた術後感染症対策. MRSA" 南山堂, 東京, 7 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanimura H: "The present circumstances and problems in clinical evaluation of new antibacterial dlugs" Jpn.J.Chemother. 45. 792-796 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanimura H: "The cause of the hepatolithiasis from a component analysis." Biliary Tract. 11. 237-248 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Uchiyama K: "The features of Japanese cholecystolithiasis and asymptomatic gall stone" Tan to Sui.19. 275-278 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Uchiyama K: "Endoscopic sphincterotomy" Gastroenterogy. 21. 798-801 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsunoda T: "Tumor immunity-Recent advances of clinical trials for cancer vaccine" Cancer Treatment and Host. 10. 99-104 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsunoda T: "New strategy for tumor specific immunotherapy using tumor antigen peptides" Biotherapy. 12. 578 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita H: "Investigation of new lipid microsphere-encapsulated antifungal drug" J.Wakayama Med.Soc.49. 153-165 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kontani T: "Establishment of PCR-diagnosis for Candida albicans and clinical investigation for fungal translocation" J.Wakayama Med.Soc. 49. 209-222 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 谷村 弘: "新抗菌薬臨床評価の現状と問題点-新しいガイドラインの位置づけ.外科領域感染症" 日本化学療法学会雑誌. 45(9). 792-796 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 谷村 弘: "成分分析からみた肝内結石の成因" 胆道. 11(3). 237-248 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 内山和久: "日本人胆石の特徴と無症状胆石" 胆と膵. 19(4). 275-278 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 内山和久: "内視鏡的乳頭括約筋切開術" 消化器外科. 21(5). 798-801 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 角田卓也: "腫瘍免疫-最近の進歩ペプタイドと腫瘍免疫「臨床編」" 癌治療と宿主. 10(1). 99-104 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 角田卓也: "ペプタイドを用いる癌特異的免疫療法の飛躍的成績向上のための新戦略" Biotherapy. 12. 578 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 谷村 弘: "外科と栄養.栄養・健康科学シリーズ,臨床栄養学" 南江堂,東京, 14 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 谷村 弘: "起炎菌からみた術後感染症対策.MRSA" 南山堂,東京, 7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 谷村 弘: "肝内結石症の手術" 手術. 51・7. 939-946 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷村 弘: "真菌感染症.私の処方とその解説.器具留置に伴う真菌血症" 今月の治療. 5. 946-948 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷村 弘: "消化器外科手術の合併症とその対策 術前・術中・術後の看護ポイント.薬剤投与に関連した合併症.抗菌薬の有害事象" オペナーシング('97秋季増刊). 151. 221-225 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷村 弘: "胆管結石に対する治療法の選択" 消化器外科展望[1997年7月〜1997年9月]. 36-41 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷村 弘: "術後感染発症阻止抗菌薬の臨床評価に関するガイドライン(1997年版)" 日本化学療法学会雑誌. 45・7. 553-641 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 内山和久: "総胆管結石の種類と治療変遷の現状" 肝胆膵. 35・5. 561-568 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 内山和久: "総胆管切石術" 外科治療. 76・5. 878-882 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 角田卓也: "ペプタイドと臨床免疫「臨床編」" 治療と宿主. 10. 99-104 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 角田卓也: "最近のグラム陽性球菌の薬剤耐性化(第1報),近畿地区におけるMRSAに関するアンケート調査成績" 日本感染症学会雑誌. 71. 1-5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 角田卓也: "グラム陽性球菌の薬剤耐性化における最近の動向(第2報),近畿地区におけるペニシリン耐性肺炎球菌に関するアンケート調査成績" 日本感染症学会雑誌. 71. 6-10 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷村 弘: "胆石症(肝内結石を含む)「今日の治療指針1997年版」" 医学書院,東京, 2 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷村 弘: "脂肪乳剤ハンドブック" ジェフコ-ポレーション,東京, 24 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷村 弘: "NEW外科学,改訂第2版,胆嚢" 南江堂,東京, 22 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷村 弘: "ベッド管理のてびき,脂肪はどうして静注できるか" 医歯薬出版,東京, 5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takuya Tsunoda: "Clonal and functional analysis for the augmentation of tumor-infiltrating lymphocytes by interleukin 4" British Journal of Cancer. 74. 1085-1089 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takuya Tsunoda: "Interleukin 4 inhibits LAK and inital phase TIL activity via interleukin 2 receptor" International Journal of Oncologu. 7. 1117-1121 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masaji Tani: "Generation of CD4+ cytotoxic T lymphocytes stimulated by immobilized anti-CD3 monoclonal antibody and interleukin-2 cancer patients" International Journal of Cancer. 60. 802-807 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shizuma Mizobata: "Fibronectin promotes the proliferation of cytotoxic T lymphocytes generated from cancer patients" British Journal of Cancer. 74. 1598-1604 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 谷村 弘: "原因不明の外科感染症" 日本外科学会雑誌. 97. 1066-1071 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 角田卓也: "消化器外科における深在性真菌症" MEDICAMENT NEWS. 1500. 6-7 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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