| Co-Investigator(Kenkyū-buntansha) |
TSUNODA Akira School of Medicine, Showa University, Lecturer, 医学部, 講師 (10183485)
SHIBUSAWA Miki School of Medicine, Showa University, Associate Professor, 医学部, 助教授 (90138496)
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| Budget Amount *help |
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Research Abstract |
We have investigated colorectal cancers by Comparative Genomic Hybridizaton(CGH) and Fluorescence in situ hybridization(FISH).
1. We investigated the cell line of SRM that was known the amplification of c-myc previously, by CGH.In SRM, it recognized the amplification in 3q, 8q and 11q and the loss in 8p.
2. In the investigation in 12 cases in colorectal cancer, it recognized the amplification in 20p(6/12) and 20q(7/12) and the loss in 18q(8/12) and 22q(5/12).
3. A colorectal cancer with liver metastasis appears about 10% and relates to the prognosis. We investigate a genetic changes of a colorectal cancer with liver metastasis by Comparative Genomic Hybridization(CGH) In this study, we analyzed the abnormal chromosomes in nine cases with colon cancer with liver metastasis and 19 cases without liver metastasis. A gain in chromosomes in colorectal cancers with liver metastasis of 8q(5/9), 20p(5/9), 20q(8/9) was observed. In colorectal cancers without liver metastasis gains in chromosomes of 8q(2/19), 20p(6/l9), 20q(5/19) were observed.
4. In FISH, the gain in a number of copy in 7, 17, 18 and 20. Especially, the gain in a number of copy of 20 showed 84.6%.
Therefor, CGH and FISH are the useful analysis in a genetic changes of a colorectal cancer. In CGH, a colorectal cancer with liver metastasis is concerned with the amplification in 20q.
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