| Project/Area Number |
08457334
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
HIOKI Koshiro Kansai Med.Univ.School of Medicine, Professor, 医学部, 教授 (60077641)
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| Co-Investigator(Kenkyū-buntansha) |
OKUSA Tokio Kansai Med.Univ.School of Medicine, Assistant, 医学部, 助手 (80247907)
TANAKA Kanji Kansai Med.Univ.School of Medicine, Lecturer, 医学部, 講師 (90221390)
NAKANE Yasushi Kansai Med.Univ.School of Medicine, Associate Prof., 医学部, 助教授 (60155778)
YAMAMICHI Keigo Kansai Med.Univ.School of Medicine, Assistant, 医学部, 助手 (70291804)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1996: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | Gastric Cancer / Biopsy Specimen / CD44 Variant / PT-PCR / Prognosis |
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| Research Abstract |
CD44 is a cell surface adhesion molecule involved in cell-cell and cell-matrix interactions. CD44 exists as a standard form and as multiple isoforms, each generated by alternative splicing of up to 10 variant exons (termed vl-vl0) encoding parts of the extracellular domain. Expression of CD44 and its variants are associated with cancer progression and metastasis. Using molecular biological examination, we examined the expression of the standard and variant (v6 and v9) forms of CD44 mRNA in operated specimens from 73 cases of gastric cancer. We determined the ratio of mRNA expression in cancer tissue to normal tissue (T/N ratio) and evaluated the correlation of the ratio with clinicopathologic features, tumor progression and prognosis. The expression level of standard form of CD44 rnRNA correlated with only peritoneal dissemination, and that of CD44v9 mRNA did not significantly correlate with any clinicopathologic factor. The expression level of CD44v6 mRNA was significantly higher in patients with lymph node metastasis and liver metastasis. In 48 curatively resected patients, the expression level of CD44v6 mRNA correlated with the site of recurrence. Furthermore, there was a significant survival advantage in patients with lowexpression of CD44v6 mRNA (T/N<l.9) compared with those with high-expression (T/N*1.9). We also examined the expression level of CD44v6 mRNA in endoscopic biopsy specimens from 30 cases of gastric cancer. The expression of CD44v6 mRNA in biopsy specimen was similar to that in operated specimenfrom same patient. These results suggest the expression level of CD44v6 mRNA may be a potential prognostic indicator and may be useful as a preoperative predictor for distant metastasis and recurrence in patients with gastric cancer.
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