Analysis of the mechanism of inhibition of liver metastasis from colon cancer ------The role of Interleukin 2 receptor of the liver sinusoidal mononuclear cells
| Project/Area Number |
08457337
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | TAZUKE KOFUKAI MEDICAL RESEARCH INSTITUTE. |
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Principal Investigator |
NAKAMURA Yoshiaki Tazuke Kofukai Medical Research Institle, Department II of Oncology, Laboratory staff, 医学研究所・第2研究部, 研究員 (50281605)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKE Masayuki Tazuke Kofukai Medical Research Institule, Department V of Oncology, Laboratory, 医学研究所・第5研究部, 部長 (90250076)
TAKABAYASHI Arimichi Tazuke Kofukai Medical Research Institute, Department II of Oncology, Laboratory, 医学研究所・第2研究部, 研究主幹 (00226911)
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| Project Period (FY) |
1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1996: ¥7,600,000 (Direct Cost: ¥7,600,000)
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| Keywords | colon cancer / liver metastasis / liver sinusoidal monounclear cell / CD3 / CD56 / IL-2 receptor / immuno-chemotherapy / CEA |
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| Research Abstract |
[Purpose] in this study, we studied the characters of SMC and PBMC,based on the hypothesis that the immune system managed by sinusoidal mononuclear cells (SMC) was damaged in the patient bearing metastatic liver cancer (MLC).
[Materials and methods] We analyzed the surface antigens on SMC and PBMC by flowcytometry in seven liver specimens taken from hepatectomized patients with MLC.The patients were aged from 35 to 80,4males and 3 females. Primary lesions were cecum (2), ascending colon (2) and 3 sigmoid colon (3). In the control group, we analized PBMC of healthy candidates. Response against IL-2 was assesed by MTT assay.
[Results] In MLC patients, CD3 (+) T cell decreased to 30 % (50-60% : normal) in PMBC and 40% (50% : normal) in SMC.CD16 (+) NK cells increased to 25-69% (20% : normal). Abnormal CD4/CD8 ratio in PBMC were observed in three cases, who showed high CEA value.CD8 and CD56 double positive cells were decresed below 30% in comparison with normal SMC (50%). IL-2R alpha chain was induced, but IL-2R beta chain was not recognized even after IL-2 stimulation.
[Conclusion] In MLC,CTL cells were decreased in SMC,especially in the high CEA value patients, showng the possibility of lower immunological defense ability than normal. The lower responsiveness to IL-2 in CTL was also recognized. It may be effective that the immuno-chemothrapy by injection via hepatic artery or portal vein using anti-cancer drugs and additional IL-2. This therapy will increase the CTL cells in SMC and make a good control in IL-2 R system, affecting the excellent results for the therapy in liver metastasis patients.
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Report
(2 results)
Research Products
(3 results)
