PREVENTION OF CALCIFICATION IN GLUTARALDEHYDE-TREATED BIOPROSTHESIS
| Project/Area Number |
08457343
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | AKITA UNIVERSITY |
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Principal Investigator |
SEKINE Satoshi AKITA UNIVERSITY,DEPARTMENT OF CARDIOVASCULAR SURGERY ASSOCIATE PROFFESOR, 医学部, 助教授 (80206630)
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| Co-Investigator(Kenkyū-buntansha) |
JYOTIRMAY Chanda AKITA UNIVERSITY,DEPARTMENT OF CARDIOVASCULAR SURGERY ASSISTANT PROFESSOR, 医学部, 助手 (30292387)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | CALCIFICATION / HEPARIN / GULTARALDEHYDE / porcine pulmonary valve / porcine aortic valve / heparin / anticalcification / bioprostheses |
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| Research Abstract |
The problem of calcification in porcine aortic (AVs) and pulmonary (PVs) valves and its relationship to gultaraldehyde (GA) is of current interest. Weproposed an anticalcification treatment to develop non-calcifying porcine AVs and PVs.
Porcine AVs and PVs were cross-linked in GA.Partially degraded heparin was coupled to the GA-treated AVs and PVs through intermediate surface-bound substrate containing amino groups. Control AVs and PVs were cross-linked in 0.625% GA but had no heparin coupling. All specimens were implanted subdermally in 3-week old rats for 5 months for calcification studies.
Control AVs (Ca, 233.69+42.61mg/g) and PVs (Ca, 181.48+4.06) were severely calcified. Coupling of partially degraded heparin revealed complete prevention of calcification in GA-treated AVs (Ca, 0.73+0.27mg/g) and PVs (Ca, 1.125+0.22mg/g) implanted snbcutaneously in weanling rats for 5 months.
The proposed anticalcification treatment is effective in preventing calcification of GA-treated AVs and PVs implanted subcutaneously in weanling rats for 5 months.(Ann Thorac Surg 1997 ; 64 : 1063-6)
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Report
(3 results)
Research Products
(6 results)
