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グリオーマにおけるテロメレース活性およびテロメア長の臨床応用への検討

Research Project

Project/Area Number 08457366
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionTOTTORI UNIVERSITY

Principal Investigator

OSHIMURA Mitsuo  Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (20111619)

Co-Investigator(Kenkyū-buntansha) KAMITANI Hideki  Tottori University, Faculty of Medicine, Research Associate, 医学部, 助手 (70194967)
HORI Tomokatsu  Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (60010443)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsGlioma / Telomerase / Telomere / Diagnosis / FISH / 予後
Research Abstract

Telomeres progressively shorten with age in somatic cells in culture and in vivo because DNA replication results in the loss of chromosome ends. Normal cells in vivo and in vitro have a limited lifespan, whereas tumor cells can often grow indefinitely. Telomerase, a ribonucleoprotein which extends shortened chromosomal ends is re-expressed in most tumors. However, there are only a few reports on telomere length and telomerase in intracranial tumors. Thus, we examined telomere length and telomerase activity in gliomas, and FISH analysis of telomeres interphase nuclei for its usefulness as tumor markers. In this study, the telomere restriction fragments and telomerase activity were studied in 50 patients with various grades of gliomas. Telomerase activity was frequently positive in glioblastomas (86%), and telomere lengths were similar or shorter, compared with those in normal tissues. Some cases were repeatedly examined and had the positive telomerase activity during a tumor progression. In one case, however, tumor cells initially were negative for the activity and become positive, accompanied with a significant TRF shortening. Unlike most malignant tumors, longer TRFs than those in normal tissues were observed in 35% of the samples. Most of them were of astrocytoma (Grade 3). Fluorescence in situ hybridization showed that cases with longer telomerase-negative, and shorter (telomere-positive) telomeres can be detected, suggesting that FISH analysis in interphases and paraffin sections are useful for clinical applications.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 押村光雄,田中宏美,久郷裕之: "テロメラーゼと細胞老化多経路説" 細胞工学. 15. 1541-1547 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 田中宏美,押村光雄: "テロメア・テロメレースと細胞老化" 生体の科学. 47. 547-552 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 押村光雄,清水素行: "細胞工学的手法によるテロメラーゼ抑制" Surgery Frontier. 4. 43-49 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takubo,K.,Nakamura,K.,Izumiyama,N.et al.: "Telomerase activity in esophageal carcinoma" J.Sur.Oncol.66. 88-92 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oshimura,M.and Barrett,J.C.: "Multiple pathways to cellular senescence: Role of telomerase repressors" Euro.J.Cancer. 33. 710-715 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okayasu,I.,Osakabe,T.,Fujiwara,M.et al.: "Significant correlation of telomerase activity in thyroid papillary carcinomas with cell differentiation,proliferation and extrathyroidal extension" Jpn.J.Cacner Res.88. 965-970 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi,C.,Miyagawa,I.,Kumano,S.et al.: "Detection of telomerase activity in prostate cancer by needle biopsy" Euro.Urol.32. 494-498 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Katoh,M.,Katoh,M.,Kameyama,M.et al.: "A repressor function for telomerase activity in telomerase-negative immortal cells" Mol.Carcinogen.21. 17-25 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uejima,H.,Shinohara,T.,Nakayama,Y.et al.: "Mapping a novel cellular senesoence gene to human chromosome 2q37 via irradiation microcell-mediated chromosome transfer" Mol.Carcinogen.in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka,H.,Shimizu,M.,Horikawa,I.et al.: "Evidence for a putative telomerase repressor gene on the 3p14.2-p21.1 region" Genes,Chrom.& Cancer. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 久郷裕之,押村光雄: "臨床染色体診断法" 金原出版社(古圧敏行監修), 5 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takubo, K., Nakamura, K., Izumiyama, N.et al.: "Telomerase activity in esophageal carcinoma." J.Surg.Oncol.66. 88-92 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oshimura, M.and Barrett, J.C.: "Multiple pathways to cellular senescence : Role of telomerase repressors." Euro.J.Cancer. 33. 710-715 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okayasu, I., Osakabe, T., Fujiwara, M.et al.: "Significant correlation of telomerase activity in thyroid papillary caricnomas with cell differentiation, proliferation and extrathyrokdal extension." Jpn.J.Cancer. 88. 965-970 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi, C., Miyagawa, I., Kumano, S.et al.: "Detection of telomerase activity in porstate cancer by needlebiopsy." Euro.Urol.32. 494-498 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Katoh, M., Katoh, M., Kameyama, M.et al.: "A repressor function for telomerase activity in telomerase-negative immortal cells." Mol.Carcinogen.21. 17-25 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uejima, H., Shinohara, T., Nakayama, Y.et al.: "Mapping a novel cellular senescence gene to human chromosome 2q37 via irradiation microcell-mediated chromosome transfer." Mol.Carcinogen.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka, H., Shimizu, M., Horikawa, I.et al.: "Evidence for a putative telomerase repressor gene on the 3p14.2p21.1 region." Genes, Chrom.& Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oshimura,M. and Barrett,J.C.: "Multiple pathways to cellular senescence : Role of telomerase repressors" Euro.J.Cancer. 33. 710-715 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okayasu,I., Osakabe,T., Fujiwara,M.et al.: "Significant correlation of telomerase activity in thyroid papillary carcinomas with cell differentiation ,proliferation and extrathyroidal extension" Jpn.J.Cancer Res.88. 965-970 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takahashi,C., Miyagawa,I., Kumano,S.et al.: "Detection of telomerase activity in prostate cancer by needle biopsy" Euro.Urol.32. 494-498 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Katoh,M., Katoh,M., Kameyama,M.et al.: "A repressor function for telomerase activity in telomerase-negative immortal cells" Mol.Carcinogen.21. 17-25 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uejima,H., Shinohara,T., Nakayama,Y.et al.: "Mapping a novel oellylar senesoence gene to human chromosome 2q37 via irradiation microcell-mediated chromosome transfer" Mol.Carcinogen.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka,H., Shimizu,M., Horikawa,I.et al.: "Evidence for a putative telomerase repressor gene on the 3p14.2-p21.1 region" Genes,Chrom.& Cancer. (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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