| Project/Area Number |
08457385
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
HAIDA Nobuhide Kanazawa University School of Medicine Professor, 医学部, 教授 (00135089)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Toshiaki Kanazawa University School of Medicine Instructors, 医学部, 助手 (00220319)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | skeletal muscle / regeneration / aging / exercise / myotube / satellite cell / tenotomy / 運動 / DNA / 塩酸ブピバカイン / 筋線維 / 筋収縮特性 / 組織学 |
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| Research Abstract |
The following experiments were done to study the regenerative ability though how to modified by aging, continuos tension, and exercise to rat skeletal muscle.
1. Change by aging. Bupivacaine was injected into the anterior tibial muscles of 2-year-old rats. The induced degeneration-regeneration was slow, taking 3 weeks to completion (1 week in young rats). The reconstituted muscle showed small fiber size, an increased number of internal nuclei.
2. Influence of synergistic tenotomy. Bupivacaine was injected into the extensor digitorum longus muscle after tonotomy of anterior tibial muscle. Tenotomized muscle gained maturation for 5-6 weeks. The regenerating process was slower in the tenotomized muscle than in the control muscle.
3. Effect of exercise on regeneration. The effect of exercise during regeneration process on satellite cell activation was studied. It was found that satellite cells in these muscles were synthesizing DNA 30 h after bupivacaine treatment. Respectively, this was 18 h earlier than found on non-exercising muscle. Satellite cells are activated earlier and muscle fibers mature more rapidly.
It was confirmed that the constant contraction and relaxation of the exercising muscles was effective in the restoration and the promotion of maturity on regenerating muscle.
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