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Evaluation of preferential motor reinnervation by measuring choline acetyltransferase activity and by Karnovsky staining

Research Project

Project/Area Number 08457394
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionUniversity of the Ryukyus

Principal Investigator

KANAYA Fuminori  University of the Ryukyus, School of Medicine, Associate Professer, 医学部, 助教授 (90233866)

Co-Investigator(Kenkyū-buntansha) FUTENNMA Chojo  University of the Ryukyus, School of Medicine, Assistant Professer, 医学部・附属病院, 助手 (20264492)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥5,600,000 (Direct Cost: ¥5,600,000)
Keywordsselective motor reinnervation / choline acethltransferase activity / Karnovsky staining / misdirection
Research Abstract

A hundred-eight Wister rats were used to examine whether preferential motor fiber regeneration exists. Rat femoral nerve bifurcates into motor branch (quadriceps branch) and sensory branch (saphenous nerve). Femoral nerve was sectioned just proximal to the bifurcation and both nerve stumps were inserted into silicone tube leaving 2mm gap (2mm gap group) and 4mm gap (4mm gap group) between stumps. Right femoral nerve was repaired without rotation (no rotation group) and left femoral nerve was repaired with 180 degrees rotation (rotation group). At 4,8,16,24,36,48 weeks after surgery, nerve specimens were harvested from both motor and sensory branches 5mm distal from distal nerve stumps. Choline acetyltransferase (CAT) activity of these specimens and those from normal nerves were measured. Also frozen sections of these specimens were stained with Karnovsky staining in order to see the localization of acetylcholine esterase, which is specific to motor axon, at 4,8,12,24 weeks after surgery. Regenerated motor axons were counted.
At 2mm gap group, CAT activity in motor branch became significantly higher than that in sensory branch at 4,8,16,24 weeks in no rotation group and at 8 weeks in rotation group (p<0.05). At 4mm gap group, CAT activity in motor branch became significantly higher than that in sensory branch at 4,8,16,24,36 weeks in no rotation group and at 8,16,36weeks in rotation group (p<0.05). Histrogic examination showed no specific motor exon staining was seen 4 weeks after surgery. Regenerated motor axons started to show staining in both motor and sensory branches after 8 weeks in each group. Number of regenerated exons increased with time in motor and sensory branches in no rotation and rotation groups at 2mm gap group, and was not different between motor and sensory branches. This result and significant higher CAT activity in motor branch suggests that motor axons regenerated into motor branch matured more than those regenerated into sensory branch.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 普天間 朝上: "再生運動神経と知覚神経の識別法に関する実験的検討" 日本マイクロサージャリー学会誌. 7・1. 28-33 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 普天間 朝上: "酵素活性(CAT活性)を用いた神経線維の選択的再生能の検討-シリコンユ-ブ架橋(2mm間隙)神経修復のCAT活性-" 日本手の外科学会誌. 11・3. 434-437 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 普天間 朝上: "CAT活性を用いた神経線維の選択的再生能の検討(第4報)" 日本手の外科学会誌. 12・3. 455-458 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 普天間 朝上: "CAT活性とKarnovsky染色を用いた神経線維の選択的再生能の検討" 末梢神経. 6・1. 43-49 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chojo Futenma: "Differentation of Regenerated Motor and Sensory Fiber by Karnovsky Staining and CAT Activity" J.Jpn.S.R.M. vol.7-No.1. 28-33 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chojo Futenma: "Evaluation of Selective Motor Reinnervation by Measuring Choline Acetyltransferase Activity" J.Jpn.Soc.S urg.Hand. vol.11-No.3. 434-437 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chojo Futenma: "Evaluation of Selective Motor Reinnervation by Measuring Choline Acetyltransferase Activity" J.Jpn.Soc.S urg.Hand. vol.12-No.3. 455-458 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chojo Futenma: "Evaluation of preferential motor reinnervation by measuring choline acetyltransferase activity and by Karnovsky staining" Peripheral Nerve. vol.6-No.1. 43-49 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 普天間朝上: "再生運動神経と知覚神経の識別法に関する実験的検討" 日本マイクロサージャリー学会誌. 7・1. 28-33 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] 普天間朝上: "酵素活性(CAT活性)を用いた神経線維の選択的再生能の検討-シリコンユ-ブ架橋(2mm間隙)神経修復のCAT活性-" 日本手の外科学会誌. 11・3. 434-437 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] 普天間朝上: "CAT活性を用いた神経線維の選択的再生能の検討(第4報)" 日本手の外科学会誌. 12・3. 455-458 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] 普天間朝上: "CAT活性とKarnovsky染色を用いた神経線維の選択的再生能の検討" 末梢神経. 6・1. 43-49 (1995)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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