| Project/Area Number |
08457395
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | YOKOHAMA CITY UNIVERSITY |
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Principal Investigator |
KOSHINO Tomihisa YOKOHAMA CITY UNIVERSITY SCHOOL OF MEDISINE DEPARTMENT OF ORTHOPAEDIC SURGERY,PROFESSOR, 医学部, 教授 (90046029)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Naotaka YOKOHAMA CITY UNIVERSITY SCHOOL OF MEDISINE DEPARTMENT OF ORTHOPAEDIC SURGERY,IN, 医学部, 講師 (90235119)
SAITO Tomoyuki YOKOHAMA CITY UNIVERSITY SCHOOL OF MEDISINE DEPARTMENT OF ORTHOPAEDIC SURGERY,AS, 医学部, 助教授 (30170517)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | osteoarthritis of the knee / degenerated cartilage / regenerated cartilage / high tibial osteotomy / classification of degenerated cartilage / classification of regenerated cartilage / 変性軟骨分類法 |
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| Research Abstract |
High tibial osteotomy (HTO) was performed 127 osteoarthritic knees. Regeneration of the articular cartilage after HTO was observed and classified.
Macroscopic findings at the time of plate removal were compared to those of the initial HTO.The averaged interval between these two operations was 25 months. The biopsy specimens were taken from the medial femoral condyle in 28 knees.
At the time of the initial high tibial osteotomy the weight bearing portion of the articular surface was markedly degenerated in the medial femoral condyle. At the time of the plate removal, regeneration of the articular cartilage in the medial femoral condyle classified into the stages as Stage 0 (no regenerative change), Stage 1 (pink and yellowish fibrillation), Stage 2 (white fibrillation scattering), Stage 3 (partial coverage with white fibrillation), Stage 4 (overgrowth of white fibrocartilage), Stage 5 (even coverage with white fibrocartilage).
The biopsy specimens showed different cell types and staining patterns between superficial zone with spindle shaped fibrocartilage-like cells and middle to deep zone with round shape distributed hyaline cartilage-like cells. Anti type collagen was observed in the cyteplasma and pericellar matrix of fibrocartilage-like cells while Safranin-0, anti-type collagen and S-100 protein was observed those area of hyaline cartilage-like cells. In the hybridization for mRNA of IGF-1 revealed that cells in the surface of regenerated cartilage were positive.
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