| Project/Area Number |
08457429
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyoto Prefectural University of Medeicine |
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Principal Investigator |
WATANABE Hiroki Kyoto Prefectural University of Medeicine, Professor, 医学部, 教授 (10004839)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Makoto Kyoto Prefectural University of Medicine, Assistant, 医学部, 助手 (60244597)
KOJIMA Munekado Kyoto Prefectural University of Medeicine, Assistant Professor, 医学部, 助教授 (80178269)
SAITOH Masahito Meiji University of Oriental Medicine, Professor, 鍼灸学部, 教授 (70106417)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | renel cell carcinoma / DNA ploidy / selective renal tumor biopsy / Flow Cytometry / AgNORs / PCNA |
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| Research Abstract |
The object of this study is to evaluate the efficacy of selectiverenal tumor biopsy in patients with renal cell carcinoma. The accuacy of histological diagnosis and DNA content of biopsies in predicting those of surgical specimens was assessed.
From 1985 to 1994,41 patients with renal cell carcinoma under went selectiverenal tumor biopsy before partial or radical nephrectomy. DNA content of renal cell carcinoma in 41 patients in biopsy specimens and surgical specimens were examined in paraffin blocks with static cytofluorometry (SCM) and Flow cytometry (FCM).
In 39 out of 41 cases, by SCM,as well as in 37 out of 41 cases by FCM,DNA content in biopsy could be analyzed. Findings of selective renal tumor biopsy for RCC coincided with those of surgical specimen in 27/39 (accuracy 69%, kappa 0.398) in cell types, 29/39 (accuracy 74%, kappa 0.432) in structural type, 26/39 (accuracy 67%, kappa 0.341) in gradings and 27/39 (accuracy 69%, kappa 0.532) in ploidy patterns.
It was assumed that DNA histograms might help the histological diagnosis by biopsy.
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