| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1996: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Research Abstract |
(Purpose)
Blood coagulationfactors, Fibrinolysis, Kinin-Kallikrein System, and PHG(Platelet hemostatic capacity)were measured in blood from early delivery and normal delivery during l-3 trimester.
Since fibrin plays an important role in maintaining the integrity of the uteroplacental circulation, it is very important to understand the role that the fibrinolytic inhibitors, PAl-land PAI-2 plays in the context.
Of equal importance is platelet function during pregnancy and onset of delivery. Platelet hemostatic capacity(PHC) was determined by a PFA-100, which is microprocessor-controlled instrument/test cartridge system that stimulates platelet-based primary hemostasis, thereby representing a new in vitro bleeding time test.
(Methods)
20 normal delivery and 17 early delivery were tested during 1st trimest-er, 2nd trimester, 3rd trimester and at the onset of labor. Additionally 16 none pregnant women were investigated as a control group. Markers of in vivo hemostatic activation , fibrinolytic parameters were measured as follows. : TAT, F_<1+2>, PAI-1, PAI-2, were analyzed using the following commercial kits : TAT(Thrombin-Antithrombin III Complex) (Dade-Behring, Marburg, Germany) F1+2(Dade-Behring, Marburg, Germany), PAI- 1(Plasminogen activator inhibitor 1) and PAI-2(Plasminogen activator inhibitor 2) (Biopool, Umea, Sweden)
(Results)
1) PFA-100 analysis was performed with collagen-epinephrine(PCE) and with collagen-APP cartridges. (PCA) during normal pregnancy, especially in second trimester, both PCE(98.0+2 1.0) and PCA(76.5 + 28.6) were shortened .as well as early delivery.
2) Plasminogen activator inhibitors PM-1 and PAI-2 were both increased during normal pregnancy , especially in the second trimester. There was no significant difference between normal pregnancy and early delivery.
3)At the onset of delivery, especially in the cases of early delivery, ELT (Euglobulin-Lysis-Time) was shortened (average 210 min, 3hours and 30 minutes).
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