• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The establishment and clinnical application of a enrichment technique of fetal nucleated cells from maternal peripheral blood for prenatal diagnosis

Research Project

Project/Area Number 08457434
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionUniversity of Tsukuba

Principal Investigator

KUBO Takeshi  University of Tsukuba, Institute of Clinical Medicine, Professor, 臨床医学系, 教授 (20010267)

Co-Investigator(Kenkyū-buntansha) HAMADA Hiromi  University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (60261799)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsprenatal diagnosis / maternal blood / fetal nucleated cells / nucleated red blood cell (NRBC) / fluorescence activated cell sorting (FACS) / fluorescence in situ hybridization (FISH) / 胎児有核赤血球
Research Abstract

The purpose of this study was to establish a new prenatal diagnostic method using fetal nucleated cells in maternal peripheral blood. The NRBCs (nucleated red blood. The NRBCs (nucleated red cells) in maternal blood were separated and enriched by FACS (fluorescence activated cell sorting) technique and were analyzed using FISH (fluorescence in situ hybridization) method. The results of the study showed several new findings as follows ;
1.It was confirmed that FACS with a combination of specific antibodies had the potential to separate and enrich NRBCs from peripheral blood samples of pregnant women.
2.The frequency of fetal NRBCs in maternal peripheral blood had a tendency to decrease as the pregnancies advanced.
3.The sorted samples from pregnant women who had male fetuses were analyzed by FISH with Y-specific probe. The fetal cells in the sorted NRBCs were determined by FISH.95% of the sorted NRBCs were, however, maternal in origin.
4. In this series, the specificity of the fetal sex diagnosis was 100%, the sensitivity was 88%, the negative predictive value was 86%, and the positive predictive value was 100%.
FACS has the effective potential to separate and enrich fetal NRBCs from maternal peripheral blood. Further improvement of this new diagnostic method is needed to use in clinical genetic diagnosis.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 濱田 洋実: "母体末梢血中胎児細胞を用いた出生前診断法の実用化" 産婦人科治療. 74. 237 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satoshi, Sohda: "The proportion of fetal nucleated red blood cells in matemal blood:Estim-ation by FACS analysis" Prenatal Diagnosis. 17. 743-752 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiromi Hamada: "Clinical application of the new prenatal diagnostic method using fetal cells in maternal peripheral blood (in Japanase)" Obstetrical and Gynecological Therapy. 74 (2). 237 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satoshi Sohda: "The proportion of fetal nucleated red blood cells in maternal blood : Estimation by FACS analysis" Prenatal Diagnosis. 17 (8). 743-752 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 濱田洋実: "母胎末梢血中胎児細胞を用いた出生前診断法の実用化" 産婦人科治療. 74. 237 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoshi Sohda: "The proportion of fetal nucleated red blood red blood cells in matemal blood :Estimation by Facs and analysis" Prenatal Diagnosis. 17. 743-752 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoshi Sohda: "The Proportion of Fetal Nucleated Red Blood Cells in Matemal Blood : Estimation by FACS analysis" Prenatal Diagnosis. (in press).

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2018-10-11  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi