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Interleukin-8 is a possible angiogenic factor in endometrial cancer

Research Project

Project/Area Number 08457442
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTottori University (School of Medicine)

Principal Investigator

TERAKAWA Naoki  Tottori Univ.Dept.Obstet.Gynecol., Professor, 医学部, 教授 (90163906)

Co-Investigator(Kenkyū-buntansha) HARADA Tasuku  Tottori Univ.Dept.Obstet.Gynecol., Assistant Professor, 医学部・附属病院, 講師 (40218649)
MINAGAWA Yukihisa  Tottori Univ.Dept.Obstet.Gynecol., Assistant Professor, 医学部・附属病院, 講師 (70190692)
KIGAWA Junzo  Tottori Univ.Dept.Obstet.Gynecol., Assistant Professor, 医学部, 講師 (00177784)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsEndometrial cancer / Angiogenic factor / Tumor necrosis factor a / Interleukin-8 / Stromal cells / Microvessel density / Cancer growth / Paracrine effect / 子宮内膜 / 子宮内膜症 / 細胞増殖 / IL-8 / TNFα
Research Abstract

This study was undertaken to find out the candidate of an angiogenic factor (s) in endometrial carcinoma. In recent clinical studies, we have found that the concentration of tumor necrosis factor a (TNF a) in peritoneal fluid of patients with endometriosis was increased and there was a significant correlation between the peritoneal fluid levels of TNF a and interleukin-8 (IL-8). We, therefore, examined the effects of angiogenic factors, TNF a and IL-8, on stromal cells collected from normal endometrium, endometriosis and endometrial carcinoma tissues. Addition of TNF a (0-100pg/ml) to the culture medium increased the production of IL-8 in both endometrial and endometriotic stromal cells, in a dose-dependent manner. The MTT assay showed that the number of endometriotic stromal cells increased in the presence of TNF a, but addition of anti-IL-8-antibody abolished the stimulating effect of TNF a on cell proliferation. IL-8 (200pg/ml) showed a growth-promoting effect on stromal cells of en … More dometrial carcinoma, which was 130% of control values. The expression of transcripts of TNF a receptor II and IL-8 receptor typeA were observed in each stromal cells by RT-PCR and Southern blot analysis.
Hysterectomy specimens of endometrial cancer were stained immunohistochemically for factor VIII-related antigen and anti-IL-8-antibody, and the numbers of the factor VIII-related antigen microvessels and the IL-8-stained vessels per area were counted by light microscopy. The numbers of microvessels in moderately-and poorly differentiated and those of IL-8 stained vessels in poorly differentiated carcinoma were significantly increased, suggesting that microvessel and IL-8-positive vessel densities are correlated with tumor grade. In summary, these results suggest that TNF a exerts paracrine effects on both the stromal and endothelial cells of endometrial carcinoma and stimulates the production of IL-8. The present study also raise the possibility that IL-8 may act as an angiogenic factor in endometrial cancer and promote the cancer growth. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Xiusi Cheng: "Glutathione S-transferase-π expression and glutathione concentration in ovarian carcinoma before and after chemotherapy." Cancer. 73 (3). 521-527 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tasuku Harada: "Increased interleukin-6 levels in peritoneal fluid of infertile patients with active endometriosis." Am.J.Obstet.Gynecol.176 (3). 593-597 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Terakawa: "The behavior of endometrial hyperplasia : a prospective study." J.Obstet.Gynaecol.Res.23 (3). 223-230 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yukihisa Minagwa: "Enhanced topoisomerase I activity and increased topoisomerase II α content in cisplatin-resistant cancer cell line." Jpn.J.Cancer.Res.88 (12). 1218-1223 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Junzo Kigawa: "Glutathione concentration may be a useful predictor of response to second-line chemotherapy in patients with ovarian cancer." Cancer. 82 (4). 692-702 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomio Iwabe: "Pathogenetic significance of increased levels of interleukin-8 in peritoneal fluid of patients with endometriosis." Fertil.Steril.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Terakawa: "In Vitro Biology of Sex Steroid Hormone Action" Churchill Livingstone, 184-191 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Xiusi Cheng: "Glutathione S-transferase-pi expression and glutathione concentration in ovarian carcinoma before and after chemotherapy." Cancer. 79 (3). 521-527 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tasuku Harada: "Increased interleukin-6 levels in peritoneal fluid of infertile patients wit active endometriosis." Am.J.Obstet.Gynecol.176 (3). 593-597 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Terakawa: "The behavior of endometrial hyperplasia : a prospective study." J.Obstet.Gynaecol.Res.23 (3). 223-230 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yukihisa Minagwa: "Enhanced topoisomerase I activity and increased topoisomerase II a content in cisplatin-resistant cancer cell line." Jpn.J.Cancer.Res.88 (12). 1218-1223 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Junzo Kigawa: "Glutathione concentration may be a useful predictor of response to second-line chemotherapy in patients with ovarian cancer." Cancer. 82 (4). 692-702 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomio Iwabe: "Pathogenetic significance of increased levels of interleukin-8 in peritoneal fluid of patients with endometriosis." Fetil.Steril.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Terakawa: Growth inhibition by progestins in human endometrial cancer. In Vitro Biology of Sex Steroid Hormone Action. Churchill Livingstone, 184-191 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoki Terakawa: "The behavior of endometrial hyperplasia : a prospective study." J.Obstet.Gynaecol.Res.23(3). 223-230 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yukihisa Minagwa: "The outcome of radiation therapy in elderly patients with advanced cervical cancer." Int.J.Gynecol.Obstet.58(3). 305-309 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yukihisa Minagwa: "Enhanced topoisomerase I activity and increased topoisomerase II α content in cisplatinresistant cancer cell line." Jpn.J.Cancer.Res.88(12). 1218-1223 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Junzo.Kigawa: "Glutathione concentration may be a useful predictor of response to second-line chemotherapy in patients with ovarian cancer." Cancer. 82(4). 692-702 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomio Iwabe: "Pathogenetic significance of increased levels of interleukin-8 in peritoneal fluid of patients with endometriosis." Fertil.Steril.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] J.Kigawa: "The role of neoadjuvant intraarterial infusion chemotherapy with cisplatin and bleomycin for locally advanced cervical cancer" Am J Clin Oncol. 19・3. 255-259 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Itamochi: "Antitumor Effects of Internal Iliac Arterial Infusion of Platinum Compounds in a Rabbit Cervical Cancer Model" Obstet Gynecol. 89・2. 286-290 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] X.Cheng: "Glutathione s-transferase-π and gulutathione concentration in ovarian carcinoma before and after chemotherapy" Cancer. 79・3. 521-527 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Harada: "Increased interleukin-6 levels in peritoneal fluid of infertile patients with active endometriosis" Am J Obstet Gynecol. 176(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Terakawa: "In Vitro Biology of Sex Steroid Hormone Action" Churchill Livingstone Japan, 4 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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