| Project/Area Number |
08457453
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Miyazaki Medical College (1997-1998)
Kyushu University (1996) |
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Principal Investigator |
KOMUNE Shizuo Miyazaki Medical College, Dept.of Otolaryngol., Chairman, 医学部, 教授 (10117434)
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| Co-Investigator(Kenkyū-buntansha) |
君付 隆 九州大学, 医学部, 助手 (50240908)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | Endocochlear potential / Na-K-Cl contransport / endolymph / Cochlea / 内リンパ液 / 蝸牛 / 聴覚 / トランスデューサーチャンネル / 有毛細胞 / イオンチャネル / Caイオン / 聴覚伝導路 / イオン輸送系 |
|---|
| Research Abstract |
Ouabain(1mM) applied in the perilymphatic space of the guinea-cochlea decreased the endolymphatic potential(EP) to the maximum negative level of approximately 30 mV and also did the potassium activity of endolymph([K]e). After EP reached the maximum negative potential , the perilymphatic space was perfused with Cl-free, Na-free, or 2 mM of furosemide containing 1mM of ouabait in each solution. Cl-free and Na-free inhibited the decrease of [K]e due to 1 mM of ouabain. 2mM of furosemide also inhibited the decrease of [K]e. EP did not changed during these experiments. These findings is an evidence that there exists a Na-K-Cl cotransporter in the in vivo cocblea, which is directed toward from perilymph to endolymph, ouabain-insennsitive and neutral electrically.
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