IMMUNOHISTOLOGICAL AND IMMUNOCHEMICAL STUDIES ON PROLOFERATION AND METASTATIC ACTIVITIES IN HEAD AND NECK CARCINOMAS

Research Project

Project/Area Number	08457454
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Otorhinolaryngology
Research Institution	KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
Principal Investigator	MURAKAMI Yasushi KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,DEPARTMENT OF OTOLARYNGOLOGY,PROFESSOR, 医学部, 教授 (20095657)
Co-Investigator(Kenkyū-buntansha)	YAMAGUCHI Nozomi KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,SECTION OF BIOLOGY,INSTITUTE FOR RESEAR, 医学部, 助教授 (40079752) SYOGAKI Kazuhiro KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,DEPARTMENT OF OTOLARYNGOLOGY,RESIDENT, 医学部, 助手 (60305589) KIMURA Takayasu KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,DEPARTMENT OF OTOLARYNGOLOGY,RESIDENT, 医学部, 助手 (90305590) HISA Yasuo KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,DEPARTMENT OF OTOLARYNGOLOGY,ASSOCIATE, 医学部, 助教授 (50181087) 丁剛京都府立医科大学, 医学部, 助手 (00285272) 平田行宏京都府立医科大学, 医学部, 助手 (40295655) 河田了京都府立医科大学, 医学部, 助手 (40224787) 任書燕京都府立医科大学, 医学部, 助手 (10254351) 西山康之京都府立医科大学, 医学部, 助手 (10237761)
Project Period (FY)	1996 – 1998
Project Status	Completed (Fiscal Year 1998)
Budget Amount *help	¥7,900,000 (Direct Cost: ¥7,900,000) Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000) Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000) Fiscal Year 1996: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywords	HEAD AND NECK CARCINOMA / PROLIFERATION ACTIVITY / METASTATIC ACTIVITY / PROGRESSION / SLI AND EGF-R / TYPE 4 COLLAGEN / CHROMOSOME 1p36 / RECURRENT CARCINOMAS / IP36 / E-カドヘリン / α-カテニン / カテプシンD / デスモグレイン / 4型コラゲン / MMP-2 / 頚部リンパ節転移 / 核DNA Ploidy / EGF-R / SLI
Research Abstract	Factors related to tumor proliferation and metastasis were investigated immunohistologically and immnochemically using ABC technique and ELISA.Several important findings were newly obtained, * summary of these findings are as follows ; 1) Deletion of short arm of chromosome 1p36 was found only in cases with definitely poor prognosis. 2) Progressions in histological differentiation and ploidy pattern of nuclear DNA were not observed in recurrent carcinomas, comparing with tissues obtained before primary therapy. 3) Progressions in proliferation score indicated by SLI and in expression of EGF-R were observed in recurrent carcinomas. 4) Expression of type 4 collagen at the basement membrane of cancer nest was disappeared in most cases of recurrent carcinomas. 5) These findings indicate that recurrent carcinomas apparently show progression of proliferation and metastatic activities.