• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular genetic studies on Behcet's disease

Research Project

Project/Area Number 08457466
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionYokohama City University

Principal Investigator

OHNO Shigeaki  School of Medicine, Yokohama City University Professor, 医学部, 教授 (50002382)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Satoshi  School of Medicine, Yokohama City University Assistant Professor, 医学部, 講師 (00237398)
YONEMOTO Junichi  School of Medicine, Yokohama City University Assistant Professor, 医学部・附属病院, 講師 (00240383)
SUGITA Miyuki  School of Medicine, Yokohama City University Associate Professor, 医学部, 助教授 (90235888)
ITOH Norihiko  School of Medicine, Yokohama City University Assistant, 医学部, 助手 (80264654)
UCHIO Eiichi  School of Medicine, Yokohama City University Assistant Professor, 医学部・附属病院, 講師 (70232840)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsBehcet's desease / Molecular genetics / HLA-BィイD1*ィエD15101 / MICA / MICB / Disease susceptibility gene / HLA-B*5101 / HLA / YACクローン
Research Abstract

In order to investigate the molecular genetic mechanism of Behcet's disease, we studied the HLA-B region which is located on the short arm of chromosome 6 (6p21.3). A YAC clone, Y109, was isolated from the YAC library using HLA class I specific primers. We investigated the new genes around the HLA-B which are in linkage disequilibrium with HLA-BィイD1*ィエD15101.
Y109 was found to span 600kb. This region was confirmed to contain NOB1, NOB2, NOB3, MICA and MICB, which are non-HLA genes. Investigation of MICB polymorphism by PCR-SSP showed no significant association with Behcet's disease. In contrast, the frequency of MICA-A6 was significantly higher in the patients than in the normal controls.
Furthermore, investigation of amino acids in the peptide binding region of MICA-A6 (exon 3) showed that they were all valine at position 122.
Studies on the other gene regions near the telomere on 6p showed weak association with Behcet's disease, and it was concluded that the responsible gene for Behcet's disease is located in the 230 kb between HLA-C and MICB.
Further studies on the association of Behcet's disease with these new genes are indicated. Establishment of transgenic animals of HLA-BィイD1*ィエD15101-MICA-A6 are now in progress in our laboratory, to elucidate the exact molecular genetic mechanism of this difficult disease.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Mizuki N.: "Pathogenic gene responsible for the predisposition to Behcet's disease"Intern Rev Immunol. 14. 33-48 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 杉央子: "ベーチェット病におけるCD4陽性T細胞サブセットの解析"日眼会誌. 101. 335-340 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ando H.: "Identification of a novel HLA-B allele (B^*4202) in a Saudi Arabian family with Behcet's disease"Tissue Antigens. 49. 526-528 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ota M.: "Trinucleotide repeat polymorphism within exon 5 of the MICA gene (MHC class I chain-related gene A) : allele frequency data in the nine population groups Japanese, Northern Han, Hui, Uygur, Kazakhustan, Iranian, Saudi Arabian, Greek and Italian"Tissue Antigens. 49. 448-454 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuki N.: "A strong association between HLA-B^*5101 and Behcet's disease in Greek Patients"Tissue Antigens. 50. 57-60 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuki N.: "Nucleotide sequence analysis of the HLA class I region spanning the 237-kb segment around the HLA-B and -C genes"Genomics. 42. 55-66 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 新藤裕実子: "眼疾患とMHC遺伝子"最新医学. 52. 67-72 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ando H.: "Allelic variants of the human MHC class I chain-related B gene (MICB)"Immunogenetics. 46. 499-508 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuki N.: "Pathogenic gene responsible for the predisposition to Behcet's disease"Intern Rev Immunol. 14. 33-48 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugi N.: "Analysis of the profile of CD4+ Cells in Behcet's Disease"J Jpn Ophthalmol Soc. 101:4. 335-340 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ando H.: "Identification of a novel HLA-B allele (BィイD1*ィエD14202) in a Saudi Arabian family with Behcet's disease"Tissue Antigens. 49. 526-528 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ota M.: "Trinucleotide repeat polymorphism within exon 5 of the MICA gene (MHC class I chain-related gene A) : allele frequency data in the nine population groups Japanese, Northern Han, Hui, Uygur, Kazakhustan, Iranian, Saudi Arabian, Greek and Italian"Tissue Antigens. 49. 448-454 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuki N.: "A strong association between HLA-BィイD1*ィエD15101 and Behcet's disease in Greek Patients"Tissue Antigens. 50. 57-60 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuki N.: "Nucleotide sequence analysis of the HLA class I region spanning the 237-kb segment around the HLA-B and -C genes"Genomics. 42. 55-66 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shindo Y.: "Association of ocular diseases and MHC"Saishin Igaku. 52:9. 67-72 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ando H.: "Allelic variants of the human MHC class I chain-related B gene (MICB)"Immunogenetics. 46. 499-508 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuki N.: "Pathogenic gene responsible for the predisposition to Behcet's disease" Intern Rev Immunol. 14. 33-48 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 杉 央子: "ベーチェット病におけるCD4陽性T細胞サラブセットの解析" 日眼会誌. 101・4. 335-340 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ando H.: "Identification of a novel HLA-B allele (B^*4202)in a Saudi Arabian family with Behcet's disease" Tissue Antigens. 49. 526-528 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ota M.: "Trinucleotide repeat polymorphism within exon 5 of the MICA gene (MHC class I chain-related gene A):allele frequency data in the nine population groups Japanese,Northern Han,Hui,Uvgur,Kazakhustan,Saudt Arabian,Greek and Italian" Tissue Antigens. 49. 448-454 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mizuki N.: "A strong association between HLA-B^*5101and Behcet's disease in Greek Patients" Tissue Antigens. 50. 57-60 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mizuki N.: "Nucleotide sequence analysis of the HLA class I region spanning the 237-kb segment around the HLA-B and-C genes" Genomics. 42. 55-66 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 新籐裕実子: "眼疾患とMHC遺伝子" 最新医学. 52・9. 67-72 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ando H.: "Allelic variants of the human MHC class I chain-related B gene (MICB)" Immunogenetics. 46. 499-508 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakamura S.,et al: "Insufficient expression of Fas antigen on helper T cells in Behcet's disease" Br J Ophthalmol. 80. 174-176 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizuki N.,et al: "Isolation of cDNA and genomic clones of a human Ras-related GTP-binding protein gene and its chromosomal localization to the long arm of chromosome 7,7q36" Genomics. 34. 114-118 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizuki N.,et al: "Immunogenetic studies of Behcet's disease" Rev Rhum Engl Ed. 63. 520-527 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizuki N.,et al: "HLA-C genotyping of patients with Behcet's disease in the Japnanese population" Hum Immunol. 50. 47-53 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizuki N.,et al: "Immunogenetic studies of Behcet's disease" Rev Rhum Ed Eng. 63. 520-527 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizuki N.,et al: "Pathogenic gene responsible for the predisposition to Behcet's disease" Intern Rev Immunol. 14. 33-48 (1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi