| Project/Area Number |
08457483
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
TAKADA Haruhiko Tohoku University, School of Dentistry, Department of Microbiology and Immunology, Professor, 歯学部, 教授 (30135743)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Akiko Tohoku University, Schook of Dentistry, Department of Microbiology and Immunolog, 歯学部, 学術振興会特別研究員
HANAUMI Kiyoshi Tohoku University, School of Dentistry, Department of Microbiology and Immunolog, 歯学部, 助手 (50005063)
SUGAWARA Shunji Tohoku University, School of Dentistry, Department of Microbiology and Immunolog, 歯学部, 助手 (10241639)
RIKIISHI Hidemi Tohoku University, School of Dentistry, Department of Microbiology and Immunolog, 歯学部, 講師 (70091767)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1996: ¥3,100,000 (Direct Cost: ¥3,100,000)
|
|---|
| Keywords | Oral streptococci / superantigen / Streptococcus mitis / epithelial cells / ICAM-1 / T cells / Streptococcus pyogenes / SPM / 口腔粘膜疾患 / S.pyogenes / SEB / CPA / ビリダンスレンサ球菌 / Streptococons mitis / 口腔粘膜 / UFA-1 |
|---|
| Research Abstract |
Takada et al.(J.Dent. Res., 75 : 922,1996) revealed that a superantigenic fraction F-2 from the culture ssupermatant of Streptococcus mits strain 108 endowed human peripheral blood T-cells with cytotoxic activity to human oral epithelial cells.The cytotoxic cell were mainly composed of CD8^+ cells. Pretreatment with human interferon gamma increased the sensitivity of epithelial cells to the cytotoxic of F-2-activated T-cells. The cytotoxic effects of the F-2-activated T-cells were mediated not by soluble factors as tumor necrosis factor, but the interaction between lymphocyte-function-associated amtigen-1 and intercellular adhesion molecule-1 (ICAM-1). Rikiishi et al.(FEMS Immounol. Med.Microbiol., 15 : 81,1996 & Immunology, 91 : 406,1997) isolated two novel superantigens from the supernatant of Streptococcus pyogenes type 12 strain, and designated as S.pyogenes mitogen (SPM) and mitogen-2 (SPM-2). SPM and SPM-2 had molecular weight 28,000 and 29,000, and isoelectric point (PI) of 9.2 and 6.0, respectively. Sugawara et al.(Clin.Exp.Immunol., 108 : 284,1997) examined the expression and up-regulation of cell adhesion molecles on a human colonic epithelial cell line HT-29, and the peripheral blood T lymphocyte proliferation responses to a staphylococcal superantigen SEB presentd by this cell line as compred with peripheral blood monocytes. They revealed that the superantigencic effects of SEB supported by HT-29 cells is an HLA-DR-and ICAM-1-dependent, but B7-2-and LFA-3-independent manner, whereas HLA-DR-, BF-2-, and LFA-3-dependent in the case supported by monocytes.
|
