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Receptor and signal transduction of bone morphogenetic protein (BM)

Research Project

Project/Area Number 08457488
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

OIDA Shinichiro  Biochemistry, Tokyo Medical and Dental University, assistant professor, 歯学部, 助手 (10114745)

Co-Investigator(Kenkyū-buntansha) TAMURA Masato  Kagoshima Univ.Oral Biochemistry, assistant professor, 歯学部・生化学, 助手 (30236757)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsBMP / receptor / sigunal transduction / alkaline phosphatase / シグナル伝達 / バキュロウイルス / smad / 歯の発生 / 骨形成タンパク質 / バキュロウィルス
Research Abstract

Bone morphogenetic proteins (BMPs), members of a transforming growth factor-beta (TGF-beta) superfamily, are growth and differentiation factors which induce ectopic bone formation in vivo. Although many studies on osteoinductive properties of BMPs have been conducted, little is known about the downstream components in the signal transduction machinery, beyond the mechanism of BMP receptor activation.
In this study, the osteogenic effects by osteogenic protein-1 (OP-1, BMP-7) on osteoblastic cell line MC3T3-E1 and murine stromal cell line ST2 were investigated, especially focusing on differentially expressed genes induced by OP-1 using the differential display methods.
The major findings were as follows :
1)Alkaline phosphatase specific activities of both MC3T3-E1 and ST2 increased in a dose-dependent manner by OP-1 stimulation.
2) Northern analysis showed a significant increase of osteocalcin mRNA after 7 days of OP-1 treatment.
3) 77 genes, which were differentially expressed in MC 3 T 3-E 1 and ST 2 cells, were detected on differential display fingerprints after 16-hour treatment of OP-i.
4) Some of these clones showed high levels of identical to known genes.
5) One clone called ST3v, down-regulated in ST2 cells by OP-1 stimulation, was con-firmed with quantitative RT-PCR.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] S.Oida,Y.Morotome 他: "Molecular cloning of PCR amplified BMP-related genes (GDF-5,6and7) from rat tooth cells" Journal of Hard Tissue Biology. 6(1). 16-20 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Hikiji,S.Oida他: "Direat action of nitric oxide on osteoblastic diheremtiation" FEBS Letters. 410. 238-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.N.Matsumoto,S.Oida他: "Effect of Ovaxiectomy on intestinal alkalime phosphatase expression in rats" J.Nutr.Sci.Vitaminol. 43. 529-539 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Iimura,S.Oida: "Characterization of two length cDNA for human Msx-2 from dental pulp-derived cells" DNA seguence. 8. 87-92 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hikiji, H., Shim, W.S., Oida, S., Takato, T.Koizumi, T.and Toyo-oka, T.: "Direct action of nitric oxide on osteoblastic differentiation" FEBS Letters. 410. 238-242 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oida, S., Morotome, Y., Nakamura, T.and Terashima, T.: "Molecular cloning of PCR amplified BMP-related genes (GDF-5,6 and 7) from rat tooth cells using madified p Bluescript SK+ vector" J.Hard Tissue Biolgy. 6 (1). 16-20 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto, H., Yamamoto, A., Iimura, T.Oida, S., Ezawa, I., Sasaki, S.and Goseki-Sone, M.: "Effects of overiectomy on intestinal alkaline phosphatase expression in rats" J.Nutr.Sci Vitaminol. 43. 529-539 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iimura, T., Takeda, K., Goseki, M.Maruoka, Y., Sasaki, S.and Oida, S.: "Characterization of two length cDNA for human Msx-2 from dental pulp-derived cells." DNA Sequence. 8. 87-92 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Oida, Y.Morotome他: "Molecular cloning of PCR amplified BMP-related Genes(GDF-5,6and-7)from rat tooth cells using modified pBluescript Skt vector" Journal of Hard Tissue Biology. 6. 16-20 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Goseki, S.Oida他: "Hypophosphatasia : Identification of five novel missense mutation in the tissue-nonspeciffic alkaline phosphatase" Human Mutation. (未定). 未定 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Iimura: "Characterization of two length cDNA for human Msx-2 from dental pulp-derived cells" DNA sequence. 8. 87-92 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 稲毛、大井田他: "抜歯窩の治癒過程におけるBMPの遺伝子発現" Jpn.J.Oral Biol. 40. 42-52 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Morotome, S.Oida他: "Gene expression of growth and differentiation factor-5,-6and-7 in developing bovine tooth at the root forming stage" Biochemical and Biophysical Research Communications. (未定). 未定 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Isobe,S.Oida他: "Bone morphogenetic protein encapsulated with a biodegradable and biocompatible polymer" Journal of Biomedical Materials Research. 32. 433-438 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Oida,H.Miyazaki他: "Molecular structure of the mouse amelogenin genomic DNA" DNA Sequence. 6. 307-310 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Baba,S.Oida他: "Determination of enamel protein synthesized by recombined mouse molar tooth germs in organ culture" Archs of oral Biology. 41・2. 215-219 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Yamazaki,S.Oida他: "Ectopic induction of cartilage and bone by bovine bone morphogenetic protein using a biodegradble polymeric reservoir" Journal of Biocmedical Meterials Research. 30. 1-4 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.S.Goseki,S.Oida: "Ovariectomy decreases osteogenetic activity in rat bone" J.Nutr.Sci.Vitaminol. 42. 55-67 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Iimura,S.Oida他: "Molecular and Developmental Biology of Cartilage 分担" New York Academy of Sciences, 274-277 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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