• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation of PGE synthesis by activated cytosolic phospholipase A2 and induced cyclooxygenase-2 in osteoblasts.

Research Project

Project/Area Number 08457493
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

MIYAURA Chisato  Showa University, School of Dentistry, Lecturer, 歯学部, 講師 (20138382)

Co-Investigator(Kenkyū-buntansha) JIMI Eijiro  Showa University, School of Dentistry, Assistant, 歯学部, 助手 (40276598)
UDAGAWA Nobuyuki  Showa University, School of Dentistry, Lecturer, 歯学部, 講師 (70245801)
SUDA Tatsuo  Showa University, School of Dentistry, Professor, 歯学部, 教授 (90014034)
片桐 岳信  昭和大学, 歯学部, 助手 (80245802)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordsprostaglandin / interleukin-1 / osteoblast / phospholipase A2 / cyclooxygenase / platelet-derived growth factor / プロスタグランジンE / 骨吸収 / インターロイキン-1 / ホスホリッパーゼA_2 / アラキドン酸
Research Abstract

The synthesis of prostaglandins (PGs) is regulated by the arachidonic acid release by phospholipase A2 (PLA2) and its conversion to PGs by cyclooxygenase (COX). In the present study, we examined the regulation of PG synthesis by interleukin (IL) -1 in mouse osteoblasts. Although IL-1 greatly enhanced COX-2 mRNA expression and its protein levels, PGE2 was not produced until 24 h.When arachidonic acid was added to osteoblasts precultured with IL-1, PGE2 was produced within 10 min.Of several growth factors tested, platelet-derived growth factor (PDGF) specifically initiated the synthesis of PGE2, which was markedly suppressed by a selective inhibitor of COX-2 (NS-398). In mouse osteoblasts, cytosolic PLA2 (cPLA2) mRNA and its protein were constitutively expressed and moderately increased by IL-1. PDGF markedly stimulated PLA2 activity, which was blocked by a cPLA2 inhibitor. The PDGF-induced activation of cPLA2 was accompanied by phosphorylation of its protein. On the other hand, IL-1 and IL-6 cooperatively induced COX-2 gene expression and stimulated PGE2 synthesis in co-culture of bone marrow cells and osteoblasts and in organ culture of new born mouse calvaria. In these culture system, FCS or endogenous bone marrow-derived growth factor were enough to activate cPLA2. These results indicated that cPLA2 activation by growth factor such as PDGF is essential for IL-1-induced and COX-2-dependent PGE synthesis in mouse osteoblasts.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Onoue, Y., et al.: "IL-13 and IL-14 inhibit bone resorption by suppressing cyclooxygenase-2-dependent prostaglandin synthesis in osteoblasts." J. Immunol.156. 758-764 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chen, Q. R., et al.: "Activation of cytosolic phospholipase A2 by platelet-derived growth factor is essential for cyclooxygenase-2-dependent prostaglandin E2 synthesis in mouse osteoblasts cultured with interleukin-1." J. Biol. Chem.272. 5952-5958 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tai, H., et al.: "Transcriptional induction of cyclooxygenase-2 in osteoblasts is involved in interleukin-6-induced osteoclast formation." Endocrinology. 138. 2372-2379 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyaura, C., et al.: "Increased B-lymphopoiesis by interleukin 7 induces bone loss in mice with intact ovarian function." Proc. Natl. Acad. Sci. USA. 94. 9360-9365 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Onoe, Y., et al.: "Expression of estrogen receptor β in rat bone." Endocrinology. 138. 4509-4512 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyaura, C., et al.: "Regulation of matrix metalloproteinases (MMP-2, 3, 9, and 13) by interleukin-1 and interleukin-6 in mouse calvaria : Association of MMP induction with bone resorption." Endocrinology, in press. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 宮浦 千里: "分子骨代謝学と骨粗鬆症" メディカルレビュー社, 10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 宮浦 千里: "最新・骨粗鬆症(印刷中)" ライフサイエンス社, (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Onoe, Y.et al.: "IL-13 and IL-4 inhibit done resorption by suppressing cyclooxygenase-2-dependnet prostaglandin synthesis in osteoblasts." J.Immunol.156. 758-764 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jimi, E., et al.: "lnterleukin-1alpha activates an NF-kB-like factor in osteoclast-like cells." J.Biol.Chem.271. 4605-4608 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chen, Q.-R.et al.: "Activation of cytosolic phospholipase A2 by platelet-derived growth factor is essential for cyclooxygenase-2-dependent prostaglandin E2 synthesis in mouse osteoblasts cultured with interleukin-1." J.Biol.Chem.272. 5952-5958 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tai, H., et al.: "Transcriptional induction of cyclooxygenase-2 in osteoblasts is involved in interleukin-6-induced osteoclast fomation" Endocrinology. 138. 2372-2379 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyaura, C.et al: "lncreased B-lymphopoiesis by interleukin 7 induces bone loss in mice with intact ovarian function." Proc.Natl.Acad.Sci.USA. 94. 9360-9365 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Onoe, Y.et al.: "Expression of estrogen receptor beta in rat bone" Endocrinology. 138. 4509-4512 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suda, T., et al.: "Regulating of osteoclast function." J.Bone Miner.Res.12. 869-879 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Udagawa, N.: "Interleukin-18 (Interferon-inducing factor) is produced by osteoblast and acts via granulocyte/macrophage colony-stimulating factor and not via interferon-gamma to inhibit osteoclast formation." J.Exp.Med.185. 1005-1012 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyaura, C.et al.: "Regulation of matrix metalloproteinases (MMP-2,3,9, and 13) by interleukin-1 and interleukin-6 in mouse calvaria : Association of MMP induction with bone resorption." Endocrinology. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chen,Q.R.,et al.: "Activation of cytosolic phospholipase A2 by platelet-derived growth factor is essential for cyclooxygenase-2-dependent prostaglandin E2 synthesis in mouse osteoblasts cultured with interleukin-1." J.Biol.Chem.272. 5952-5958 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tai,H.,et al.: "Transcriptional induction of cyclooxygenase-2 in osteoblasts is involved in interleukin-6-induced osteoclast formation." Endocrinology. 138. 2372-2379 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyaura,C.,et al.: "Increased B-lymphopoiesis by interleukin 7 induces bone loss in mice with intact ovarian function." Proc.Natl.Acad.Sci.USA. 94. 9360-9365 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Onoe,Y.,et al.: "Expression of estrogen receptor β in rat bone." Endocrinology. 138. 4509-4512 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyaura,C.,et al.: "Regulation of matrix metalloproteinases (MMP-2,3,9,and 13) by interleukin-1 and interleukin-6 in mouse calvaria : Association of MMP induction with bone resorption." Endocrinology,. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suda,T.,et al.: "The role of B-lymphopoiesis in bone loss due to sex steroid deficiency." The Endocrinologist,. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 宮浦千里: "最新・骨粗鬆症" ライフサイエンス出版(印刷中), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Onoe,Y.,et al.: "IL-13 and IL-4 inhibit bone resorption by suppressing cyclooxygenase-2-dependent prostaglandin synthesis in osteoblasts." J.Immunol.156. 758-764 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chaki,O.,et al.: "Androgen deficiency stimulates B-lymphopoiesis in bone marrow and induces bone loss in male mice : Comparison of the effects of estrogen and androgen." Osteoporosis 1996. 37-41 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chen,Q.R.,et al.: "Activation of cytosolic phospholipase A_2 by platelet-derived growth factor is essential for cyclooxygenase-2-dependnet prostaglandin E_2 synthesis in mouse osteoblasts cultured with IL-1." J.Biol.Chem.,in press. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tai,H.,et al.: "Transcriptional induction of cyclooxygenase-2 in osteoblasts is involved in interleukin-6-induced osteoclast formation." Endocrinology. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 宮浦千里: "骨粗鬆症 Q&A" 医薬ジャーナル社, 167 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 宮浦千里: "分子骨代謝学と骨粗鬆症" エストロゲン欠乏による骨代謝変化とサイトカイン, 10 (1996)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi