| Project/Area Number |
08457571
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
NAGASAKA Nobuo HIROSHIMA UNIVERSITY,SCHOOL OF DENTISTRY,PROFESSOR, 歯学部, 教授 (30064827)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Junji HIROSHIMA UNIVERSITY,SCHOOL OF DENTISTRY,RESEARCH ASSISTANT, 歯学部, 助手 (90263714)
ISHIDORI Hiroyuki HIROSHIMA UNIVERSITY,SCHOOL OF DENTISTRY,RESEARCH ASSISTANT, 歯学部, 助手 (50203013)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | primary tooth / permanent tooth / cell culture / TGF-beta / IGF / FGF / 歯髄細胞 |
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| Research Abstract |
The purpose of this study is to investigate the influence of various growth factors on differentiation and mineral formation of tooth pulp cells isolated from primary tooth pulp tissue and permanent tooth pulp tissue. 2 to 8 PDL cells from primary and permanent tooth pulp tissue were cultured in 6 cm cell culture dishes in alpha MEM medium with 50 mug/ml ascorbic acid, 10mM disodium beta-glycerophosphate for 10 days before adding 0.1 ng/ml to 1 ng/ml transforming growth factor beta (betaTGF-beta), insuine-like growth factor (IGF I), .and basic fibroblast growth factor (bFGF). The cells were harvested on Days 2 and 7 after adding growth factors. Medium was washed with phosphate buffered saline (PBS) for 3 times and collected with rubber spatula. Differentiation of tooth pulp cells was studied with Alkaline phosphatase activity and mineral formation. Alkaline phosphatase activity was investigated with Bessey-Lowry method. Mineral formation was investigated with OCPC method. Alkaline phosphatase activity of tooth pulp cells was increased with low concentration of TGF-beta and IGF I but was decreased with high concentration of TGF-beta and IGF I.Alkaline phosphatase activity of tooth pulp cells o Days 7 were increased from Day2. Mineral formation on Days 7 show little increase from Days 2. Nodule formation was not seen any conditions.
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