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Molecular mechanism of cell proliferation and appoptosis by the actions of transcription/replication factors in mammalian cells.

Research Project

Project/Area Number 08457599
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

ARIGA Hiroyoshi  Hokkaido Univ., Fac.of Pharm., Prof., 薬学部, 教授 (20143505)

Co-Investigator(Kenkyū-buntansha) ARIGA Sanae  Hokkaido Univ., Coll.of Med.Tech., Prof., 医療技術短期大学部, 教授 (90184283)
TAKRA Takahiro.Y.  Hokkaido Univ., Fac.of Pharm., Inst., 薬学部, 助手 (70197036)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1997: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1996: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsMYC / cell transformation / cell cycle / apoptosis / transcroption / DNA replication / c-myc / 転写因子 / cDNAクローニング / 癌遺伝子
Research Abstract

We have identified several proteins that interact with C-MYC.These were classified to 4 group ; factor for DNA replication (MSSP), for transcription (AMY-1, MM-1. CBF), for cell-cycle control (p21), and for apoptosis induction (Pim-1). Of the proteins, MSSP futrher interacts with DNA polymerase alpha and PCNA directly, and cdk2 indirectly. C-MYC interacts with PCNA-binding region of p21 to rescue the inhibition of DNA replication by p21. A novel protein AMY-1 interacts the N-terrninal, so called transactivation domain of C-MYC,to stimulate transcriptional activity of C-MYC.AMY-1 was translocated from cytoplasm to nucleus after G1/S transition. Pim-1 is serine/threonine kinase and its target protein was identified, PAP-1. Pim-1 also phosphorylates CDC25 whose gene is known to be a transcriptional targer of C-MYC.These results suggest that a versatile functions of C-MYC occur by a distinguished protein complex during cell cycle.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Pognonec, P: "Cross-family interaction between the bHLHZip USF and bZip Fra1 prote" Oncogene. 14. 2091-2098 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoyama, M.: "Effect of transcriptional regulatory sequences on autonomous replication" Biol.Pharm.Bull.20. 613-620 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Beppu, Y.: "Human mucus protease inhibitor in airway fluids is a potential defensiv" J.Biochem.121. 309-316 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagakubo, D.: "DJ-1,a novel oncogene product which transforms mouse NIH3T3 cells in" Biochem.Biophy.Res.Comm.231. 509-513 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taira, T.: "A novel G1/S-specific enhanccr identified in the human heat shock protein" Nucleic Acids Res.25. 1975-1983 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iida, M.: "Induction of apoptosis by MSSP,C-MYC binding proteins." Biol.Pharm.Bull.20. 10-14 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 有賀 寛芳: "癌遺伝子研究の展望2" 東京化学同人, 250 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 有賀 寛芳: "最新の分離・精製・検出法" エヌ・テイー・エス, 850 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Pognonec, P: "Cross-family interaction between the bHLHZip USF and bZip Fral protei" Orcogene. 14. 2091-29098 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoyama, M.: "Effect of transcriptional regulatory sequences on autonomous replication" Biol. Pharm. Bull.20. 613-620 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Beppu, Y.: "Human mucus protease inhibitor in airway fluids is a potential defensive" J.Biochem.121. 309-316 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagakubo, D.: "DJ-1, a novel oncogene product which transforms mouse NIH3T3 cells in" Biochem. Biophy. Res. Comm.231. 509-513 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taira, T.: "A novel G1/S-specific enhancer identified in the human heat shock protein" Nucleic Acids Res.25. 1975-1983 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iida, M.: "Induction of apoptosis by MSSP,C-MYC binding proteins." Biol. Pharm. Bull.20. 10-14 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ariga, H.: Perspective of oncogene II.Tokoy Kagaku Dojin, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ariga, H.: Method of purification and analyis.NTN, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Pogonec,P: "Cross-family interaction between the bHLHZip USF and bZip Fral proteins" Oncogene. 14. 2091-2098 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yokoyama,M.: "Effect of transcriptional regulatory sequences on autonomous replication of" Biol.Pharm.Bull.20. 613-620 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Beppu,Y.: "Human mucus protease inhibitor in airway fluids is a potential defensiv" J.Biochem.121. 309-316 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagakubo,D.: "DJ-1,a novel oncogene product which transforms mouse NIH3T3 cells in" Biochem.Biophy.Res.Comm.231. 509-513 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Taira,T.: "A novel G1/S-specific enhancer identified in the human heat shock protein 70" Nucleic Acids Res.25. 1975-1983 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iida,M.: "Induction of apoptosis by MSSP,C-MYC binding proteins." Biol.Pharm.Bull.20. 10-14 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 有賀寛芳: "癌遺伝子研究の展望2" 東京化学同人, 250 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 有賀寛芳: "最新の分離・精製・検出法" エヌ・テイー・エス, 850 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Manabe, A: "Transcriptional repression activity of N-myc protein requires phosphorylation by MAP kinase." Biochem. Biophys. Res. Comm.219. 813-823 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Higermoser, C.: "Cloning and characterization of the genomic DNA of the human MSSP gene." Nucleic Acids Res.24. 3856-3857 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iida, M.: "Induction of apoptosis by MSSP, C-MYC binding proteins." Biol. Pharm. Bull.20. 10-14 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Taira, T.: "A novel G1/S-specific enhancer identified in the human heat shock protein 70 gene" J. Biol. Chem.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsuchiya, T.: "Ku antigen binds to Alu family DNA and represses transcription." J. Biol. Chem.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maeda, J.: "AMY-1, a novel C-MYC binding protein that stimulates transcription and transforming activities of C-MYC" Nature. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2021-10-18  

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