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Elucidation of the mechanism of excitatory action of marine natural products on platelets and muscle cells

Research Project

Project/Area Number 08457603
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionTohoku University

Principal Investigator

OHIZUMI Yasushi  Tohoku University, Faculty of Pharmaceutical Sciences Professor, 薬学部, 教授 (00006355)

Co-Investigator(Kenkyū-buntansha) NAKATANI Keigo  Tohoku University, Faculty of Pharmaceutical Sciences Research Associate, 薬学部, 教務職員 (60281979)
FURUKAWA Ken-Ichi  Tohoku University, Faculty of Pharmaceutical Sciences Assistant Professor, 薬学部, 助手 (20165468)
NAKAHATA Norimichi  Tohoku University, Faculty of Pharmaceutical Sciences Associate Professor, 薬学部, 助教授 (60045804)
松永 公浩  東北大学, 薬学部, 助手 (90222306)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥5,400,000 (Direct Cost: ¥5,400,000)
Keywordsmarine products / plateles / muscle cells / Ca^<2+> / platelete aggregation / ATPase / thromboxane A_2 / 9-methyl-7-bromoeudistomin D / Ca^<2+>遊離チャネル / BKチャネル / 形態変化 / 蛋白リン酸化 / IIb-IIIa / 筋収縮
Research Abstract

In our screening program for natural products from marine organisms, useful not only for basic researches but also for the treatment of a variety of diseases, we have studies the mechanism of action of these compounds. The new monogalactopyranosylacylglyceroI was isolated from the cultured marine alga and caused an inhibition of platelet aggregation. Theonezolide-A (TZ-A), a novel polyketide macrolide caused a marked platelet shape change. Our pharmacological studies on this compound suggest that TZ-A is a useful chemical tool for clarifying the signal transduction mechanism. Zooxanthellatoxin-A (ZT-A) from a symbiotic dinoflagellate caused Ca^<2+>-or thromboxane A_2-dependent and genestin-sensitive aggregation of platelets. It is suggested that ZT-A primarily activate a protein tyrosine kinase and that the activated protein tyrosine kinase subsequently stimulates the activation of mitogen-activated protein kinase probably via PLC-gamma2 and PKC.On the other hand, ptilomycalin A has been showm to interacts competitively with ATP at the ATP binding site of Na^+, K^+-ATPase or Ca^<2+>-ATPaase, resulting in inhibition of both the enzymes. Recently, we have found that 9-methyl-7-bromo-eudistomin D,the most powerful caffeine like substances not but caffeine failed to induce a contraction of skinned smooth muscles. These data suggest that intracellular Ca^<2+> stores consist of two functional distinct types. These studies lead to the conclusion that these natural products are useful not only as pharmacological tools but also as leading compounds for new drug development.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Ohizumi, Y.et al.: "Ptilomycalin A,a novel Na+,K+ orCa2+-ATPase inhibitor,competitively interacts with ATP at its binding site" European Journal of Pharmacology. 310. 95-98 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Imaizumi, Y.et al.: "Characteristics of Ca2+-release for activation of K+ current and contractile system in some smooth muscles" Am.J.Physiol.C772-C782 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rho, M-C.et al.: "A novel inhibitor of platelet aggregation from the cyanophyceae Oscillatoria rosea (NIES-208)" Planta Medica. 62. 473-474 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rho, M-C.et al.: "The mode of rabbit pkatelet shape change and aggregation induced by theonezolide A,a novel polyketide macrolide,isolated from the Okinawan marine spongetheonella" Can.J.Physiol.Pharmacol.74. 193-199 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rho, M-C.et al.: "Involvement of phospholipase C-γ-2 in activation of mitogen-activated protein kinase and phospholipase A_2 by zooxanithellatoxin-A in rabbit platelets" J.Pharmacol.Exp.Ther.282. 496-504 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohizumi, Y.: "Application of physiologically active substances isolated from natural resources to pharmacological studies" Jpn.J.Pharmacol.73. 263-289 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohizumi, Y.et al.: "Ptilomycalin A,a novel Na^+, K+or Ca2+-ATPase inhibitor, competitively interacts wiht ATP at its binding site" European Journal of Pharmacology. 310. 95-98 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Imaizumi, Y.et al.: "Characteristics of Ca2+-release for activation of K+ current and contractile system in some smooth muscles" Am.J.Physiol.C772-C782 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rho, M-C.et al.: "A novel inhibitor of platelet aggregation from the cyanophyceae Oscillatoria rosea (NIES-208)" Planta Medica. 62. 473-474 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rho, M-C.et al.: "The mode of rabbit pkatelet shape change and aggregation induced by theonezolide A,a novel polyketide macrolide, isolated from the Okinawan marine sponge Theonella" Can.J.Physiol.Pharmacol.74. 193-199 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rho, M-C.et al.: "Involvement of phospholipase C-gamma2 in activation of mitogen-activated protein kinase and phospholipase A_2 by zooxanthellatoxin-A in rabbit platelets" J.Pharmacol.Exp.Ther.282. 496-504 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohizumi, Y.: "Application of physiologically active subsances isolated from natural resources to phamacological studies" Jpn.J.Pharmacol.73. 263-289 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohizumi, Y.et al.: "Ptilomycalin A,a novel NA+,K+ or Ca2+-ATPase inhibitor,competitievly interacts with ATP at its binding site" European Jounal of Pharmacology. 310. 95-98 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Imaizumi, Y.et al.: "Characteristics of Ca2+-release for activation of K+ current and contractile system in some smooth muscles" AM.J.Physiol.271. C772-C782 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Rho, M-C.rt al.: "A novel inhibitor of platelet aggregation from the cyanophyceae Oscillatoria rosea (NIES-208)" Planta medica. 62. 473-474 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Rho, M-C.et al.: "The mode of rabbit pkatelet shape change and aggregation induced by theonezolide A,a novel polyketide macrolide,isolated from the Okinawan marine sponge Theonella sp." Can.J.Physiol.Pharmacol.74. 193-199 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Rho, M-C.et al.: "Involvement of phospholipase C-γ2 in activation of mitogen-activated protein kinase and phospholipase A_2 by zooxanthellatoxin-A in rebbit platelests" J.Pharmacol.exp.Ther.282. 496-504 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohizumi, Y.: "Application of physiologically active substances isolated from natural resources to pharmacological studies" Jpn.J.Pharmacol.73. 263-289 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y. Imaizumi: "Characteristics of Ca^<2+> release for activation of K^+ current and contractile system in some smooth muscles." AMERICAN JOURNAL OF PHYSIOLOGY. 271. C772-C782 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.-C. Rho: "The mode of rabbit platelet shape change and aggregation induced by theonezolide-A, a novel polyketide macrolide, isolated from the okinawan marine sponge Theonella sp." CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY. 74. 193-199 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Ohkura: "Characteristics of ^<45>Ca^<2+> release induced by quinolidomicin Al, a 60-membered macrolide from skeletal muscle sarcoplasmic reticulum." BIOCHIMICA ET BIOPHYSICA ACTA. 1294. 177-182 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.-C. Rho: "A Novel inhibitor of platelet aggregation from the cyanophyceae Oscillatoria rosea" PLANTA MEDICA. 62. 473-474 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.-C. Rho: "A Novel monogalactosylacylglycerol with inhibitory effect on platelet aggregation from the Oscillatoria rosea." JOURNAL OF NATURAL PRODUCTS. 59. 308-309 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Ohizumi: "Ptilomycalin A, a novel Na^+, K^+-or Ca^<2+>-ATPase inhibitor, competitively interacts with ATP at its binding site." EUROPEAN JOURNAL OF PHARMACOLOGY. 310. 95-98 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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