| Project/Area Number |
08457611
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SEKIMIZU Kazuhisa Faculty of Pharmaceutical Sciences, Kyshu University Professor, 薬学部, 教授 (90126095)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | heat shock proteins / DNAsupercoling DNA gyrase / sigma 32 / DNA-dependent protein synthesis / DNA依存蛋白合成系 / DNAの超ら施構造 / ストレス刺激 / 大腸菌 / DNAの超らせん構造 / 熱ショック蛋白 / 熱ショック応答 / ナリジキシン酸 |
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| Research Abstract |
We previously reported that plasmid DNA in Escherichia coli relaxd when cells were exposed to stress. In this study, we examined stability and synthesis of sigma 32 in the presence of inhibitors of DNA gyrase.The result showed that the inhibitors stabilized sigma 32, and stimulated the synsthsis of the protein. We also showed that DNA relaxation stimulated synthesi of simga 32 in an in vitor system of DNA-dependent protein synthesis. Taken together, we conclude that DNA relaxation induces stability and synthesis of sigman 32 in cells. We propose that changes of DNA supercoiling in cells is a key signal for induction of heat shock proteins.
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