| Project/Area Number |
08457623
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
医薬分子機能学
|
|---|
| Research Institution | Okazaki National Research Institutes |
|---|
Principal Investigator |
SHIONOYA Mitsuhiko Institute for Molecular Science, Professor, 分子科学研究所, 教授 (60187333)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANAKA Kentaro Institute for Molecular Science, Research Assistant, 分子科学研究所, 助手 (40281589)
王子田 彰夫 岡崎国立共同研究機構, 特別協力研究員 (10343328)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1996: ¥4,300,000 (Direct Cost: ¥4,300,000)
|
|---|
| Keywords | Control of Gene Expression / Metal Complexes / Coodination Chemistry / Nucleic Acid / 遺伝子発現 / 分子認識 / 錯体合成 / 転写調節領域 / チミン塩基 / 塩基対認識 |
|---|
| Research Abstract |
We previously demonstrated that Zn^<II>-cyclen complexes bind selecively and reversibly to thymine (or uracil) base in aqueous solution at physiological pH.These zinc complexes also lowered the melting temperatures of double-stranded poly (A) ・Poly (U) and poly (dA) ・poly (dT), indicating that the double-stranded structures of the polynucleotides are destabilized by the strong interaction between these complexes and thymine (or uracil) bases. We have further studied the interactions between metal complexes and DNA duplexes containing AT-rich sequences which are often seen in transcriptional regulatory domains. For example, Zn^<II>-cyclen complex destabilized the double-stranded structure of oligomeric DNAs with a sequence of adenovirus TATA-box, and consequently lowered the melting temperature (T_m) depending on the concentration of the zinc complex and the solution pH.In contrast, Ni^<II>-and Cu^<II>-cyclen complexes raised the T_m. In addition, for the purpose of creating artificial DNAs as functionalized materials, we synthesized a novel nucleoside bearing a phenylenediamine subunit for metal coordination. While hydrogen donding is one of the primary driving forces to form double helixes in natural DNA,the artificial DNAs with this nucleobase are expected to form double stranded structures by metal coordination, in which metals may align along the helix axis. Hence, these metal aligned structures in the DNA have possibility of application to molecular wire, optical devices, and drugs, etc.
|
