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Development of a chemiluminescent reagent that recognizes guanine in nucleic acid, and its use for chemiluminescence detection of DNA

Research Project

Project/Area Number 08457640
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KAI Masaaki  Kyushu University Pharmaceutical Sciences TITLE OF POSITION (Associate Professor), 薬学部, 助教授 (00160953)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsNucleic acid / DNA / Chemiluminescence / Imaging detection / Arylglyoxalreagents / CCD camera / Selective detection / Hybridization / 高感度検出
Research Abstract

A novel reagent, 3', 4', 5'-trimethoxyphenylglyoxal has been found to specifically convert guanine bases of a nucleic acid in a neutral or alkaline phosphate solution to the corresponding chemiluminescent derivatives. The chemiluminogenic derivatization technique offers a facile method of the sensitive imaging detection for a DNA probe tailing with deoxyriboguanylic acid oligomers. A cooled charge-coupled device camera is usable to detect visually the chemiluminescence emitted from the derivatized probe that was hybridized with a target DNA fragment on a Nylon membrane. The proposed method permits the quantitative detection of the probe covalently attached with a 5'- (G) _<15>TT (G) _<15>TT deoxyoligomer at 3' terminus, indicating a detection limit of 0.1 pmol on the membrane. The detection sensitivity can be increased with larger contents of deoxyriboguanylic acid in the probe molecule.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 甲斐 雅亮: "グアニン塩基に特異的なルミネッセンス試薬の活用法" 蛋白質核酸酵素. 42(2). 777-783 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaaki Kai: "Practical use of luminogenic reagents specific for guanine-containing compounds" Protein, Nucleic acid and Enzyme (Japanese). Vol42, No5. 777-783 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 甲斐雅亮: "グアニン塩基に特異的なルミネッセンス試薬の活用法" 蛋白質 核酸 酵素. 42(2). 777-783 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 甲斐雅亮: "グアニン塩基に特異的なルミネッセンス試薬の活用法" 蛋白質核酸酵素. 42(4)(予定). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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