| Project/Area Number |
08457642
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | KANSAI MEDICAL UNIVERSITY |
|---|
Principal Investigator |
TAKAHASHI Hakuo KANSAI MEDICAL UNEVERSITY,FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (80094431)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MASUDA Midori KANNSAIMEDICAL UNEVERSITY,FACULTY OF MEDICINE,ASSISTANT, 医学部, 講師 (50173753)
KOMIYAMA Yutaka KANNSAIMEDICAL UNEVERSITY,FACULTY OF MEDICINE,ASSISTANT, 医学部, 講師 (40140264)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥5,500,000 (Direct Cost: ¥5,500,000)
|
|---|
| Keywords | ENDOGENOUS DIGITALIS / SODIUM BALANCE / BLOOD PRESSURE / CENTRAL NERVOUS SYSTEM / DIGITALIS BINDING PROTEIN / CELL CULTURE / 内因性ウアバイン / 高血圧 / 電解質代謝 / ウアバイン結合タンパク / 細胞培養 |
|---|
| Research Abstract |
We have been studying the role of central nervous system in the production of endogenous digitalislike factor (s) (EDLF) by using experimental animals for more than 15 years. Recently, we found that the adrenal medulla also contained digitalislike immunoreactivity in rats. Therefore, we thought that the cell-line of adrenomedullary origin could produce (EDLF). In fact, we found that PC-12 cells which is originated from pheochrocytoma cells produced ouabainlike immunoreactive substance, which was characterized by repeated chromatography. Furthermore, production of EDLF in this culture system was obviously but slightly augmented by a growth factor (since this evidence has not been published, we do not want to indicate the material here). In the present study period, we collected a great amount of culture medium after stimulating with the growth factor. After passing serveral kinds of elution columns, the EDLF was purified, and the fraction which contains ouabainlike immunoreactivity was analyzed by liquid chromatography with mass spectrometry. As the result, the substance was identified to be ouabain. This must be the first evidence that mammals can endogenously produce digitalis, because the other reports always have had the possibility that the identified material is of the diet origin. However, some other materials which showed the different chromatographic characteristics were there. The analysis is now going on. Furthermore, we identified the ouabain-binding protein in plasma, which was a fragment of IgG.
|
