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Membrane Protein Recognition by Ladder-shaped Polyether Compounds

Research Project

Project/Area Number 08458171
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

MURATA Michio  The University of Tokyo, Graduate School of Science, Associate Professor, 大学院・理学系研究科, 助教授 (40183652)

Co-Investigator(Kenkyū-buntansha) SASAKI Makoto  The University of Tokyo, Graduate School of Science, Research Associate, 大学院・理学系研究科, 助手 (80235267)
TACHIBANA Kazuo  The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (70142081)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywordspolyether compounds / membrane proteins / maitotoxin / ciguatoxin / ガングリオシド / 赤血球膜
Research Abstract

Marine microalgae are known to be arich source of structurally and biologically intriguing polyketides. Among those, maitotoxin (MTX) attracts attention of chemists and biochemists since the toxin possesses the largest molecular weight of 3422 Da among secondary metabolites and very potent lethality (50 ng/kg, mice, ip.). Its structure has been determined by combined use of NMR,MS/MS and synthetic chemistry. A new method based on carbon-proton spin coupling (2,3JC,H) was introduced for the configurational determination of the acyclic sidechains. Based on these data, the stereostructure of the whole molecule has been elucidated, which sheds light on its molecular mode of action. Recent findings suggest that MTX recognaizes its taget protein with the hydrophobic polycyclic ethers (the upper half of the structure), which probably interacts with the transmembrane portion of the protein.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] N, Matsumori.: "Long-range corbon・proton coupling constants for sureochemi assigument of acyclic Sereccure s.in natural products" Tetrahedron Letters. 37. 1269-1272 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K, Konoki.: "Blockade of MTX-induced Ca^<2+> in rat glioma C_6 alls by alhylamines" Journal of Natural Toxins. 5. 209-217 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M, Sasaki.: "The complete siructure of maetotoxin Ti Seercochemistry of the C1-C14 side chain." Argewandte Chemie,International Edition in English. 35. 1672-1675 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nonomura.: "The complete structure of maitotocin IIi Scereochemistry of the C135-C142 sidu chain and the absolute mfihuratlm of the entiremolecule" Angewandle Chemie,International Edition in English. 35. 1675-1678 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Konoki.: "Inhibition of maitotoxin-induced C_a^<2+> in fhex in rat glioma C_6 cells by breretoxing and sythetil fragments of maitoroxin." Jounal of Neurochemistry. 70. 409-416 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nobuaki Matsumori: "Long-range carbon-proton conpling constants for steereochemical assginment of acyclic Structures in natural products." Tetrahedron Letter. 37. 1269-1272 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Keiichi Konoki: "Blockade of MTX-induced Ca2+ influx rata glioma C6 cells by alkylamines." Journal of Natural Toxins.5. 209-217 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makoto Sasaki: "The complete structure of maitotoxin I ; Stereochemstry of the C1-C14 side chain." Angewandte Chemie, International Edition in English. 35. 1672-1675 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taro Nonomura: "The complete structure of maitotoxin II ; Stereochemistry of C135-C142 side chain and the absolute configuration of the entire molecule" Angewandte Chemie, International., Edition in English. 35. 1675-1678 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Keiichi Konoki: "Inhibition of maitotoxin-induced Ca2+ influx in rat glioma C6 cells by brevetoxins and synthetic fragments of maitotoxin" Journal of Neurochemistry. 70. 409-416 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Matsumori: "Long-range carbon・proton coupling constants for sureochemical assignment of acyclic seructures in natural products" Tetrahedron Letters. 37. 1269-1272 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Konoki: "Blockade of MTX-induced Ca_<2+> influx in rat glioma C6 cells by alhylamines" Journal of Natural Toxins. 5. 209-217 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Sasaki: "The complete seructure of maitotoxin I ; Sureochemistry of the C1-C14 side chain" Angewandte chemic, International Edition in English. 35. 1672-1675 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Nonomura: "The complete structure of maitotoxin II ; Sureochemistry of the C135-C142 side chain and the absolute configuration of the entire molecule" Angewandte Chemic, International Edition in English. 35. 1675-1678 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Konoki: "Inhibition of maitotoxin-induced Ca^<3+> influx in rat glioma C6 cells by bleretoxins and sythetic fragments of maitotoxin" Journal of Neurochemistry. 70. 409-416 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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