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STUDIES ON CELLULAR FUNCTIONS AND MECHANISMS FOR AN ANTI-ADHESIVE SIGNAL OF PROTEOGLYCAN,PG-M

Research Project

Project/Area Number 08458186
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionINSTITUTE FOR MOLECULAR SCIENCE OF MEDICINE,AICHI MEDICAL UNIVERSITY

Principal Investigator

KOJI Kimata  INSTITUTE FOR MOLECULAR SCIENCE OF MEDICINE,AICHI MEDICAL UNIVERSITY,PROFESSOR, 分子医科学研究所, 教授 (10022641)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1996: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsPG-M / versican / Anti-adhesion / Chondroitin sulfate / Annexine VI / Cell attachment assay / Chondroitin sulfate receptor / Signal transduction / Cell spreading / 抗-接着活性 / グリコカルフィン / コンドイチン硫酸レセプター / 抗一接着活性
Research Abstract

We have suggested that annexin VI functions as a cell-surface receptor for the chondroitin sulfate chains of proteoglycan, PG-M in a Ca^<2+>-dependent manner to transduce their anti-adhesive signals into the cells. Here we have investigated the possibility and mechanisms from the following two aspects : 1) We previously succeeded in synthesizing chondroitin sulfate-phosphatidyl ethanolamine lipid (CS-PE) which can be substituted for PG-M in regard to the anti-adhesive activity and have taken advantage of dishes coated with this new molecules for anti-adhesive substrata. Their anti-adhesive properties have been investigated using both the cell attachment assay and the cell spreading assay, which are aimed preferentially to analyze the interactions between cells and substrate molecules and the subsequent cytoskeletal changes to result in the cell spreading, respectively. As far as examined, cells of any different types could attach to dishes coated with fibronectin but not spread on it w … More hen doubly coated with CS-PE.In addition, cells could also attach to dishes coated with CS-PE alone. However, A431, a cell line derived from human epithelium which we previously found to lack annexin VI could hardly attach to the CS-PE-coated dish. Any cells including A431 could attach to and subsequently spread on dishes coated with glycosaminoglycan-lipids other than CS-PE.Taken together, the results suggest the involvement of annexin VI in a specific signal transduction to inhibit the subsequent cell-spreading reaction. Various reagents such as protein kinase-inhibitors, its activators, and inophors were tested to have any effects on the anti-adhesive activity and none of them gave the significant effects. 2) Changes in cellular morphology and migration activity were analyzed when cells were transfected with the vectors containing cDNAs for various alternative spliced forms of PG-M to yield the molecules having various potential attachment sites for chondroitin sulfate chains. Transgenic mouse systems were also used to examine the effects. However, we have not observed any significant differences. The expression systems are now taken into reconsideration to yield the enough expression. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] Perris, P.et al.: "Inhibitory effects of PG-H/aggrecan and PG-M/versican on avian neural crest cell migration." FASEB J. 10・2. 293-301 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takanosu, M.et al.: "Distribution of chondroitin sulphate proteoglycans and peanut agglutinin-binding molecules during bovine fetal palatine ridge formation." J Anat. 189. 109-115 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Isogai, Z.et al.: "2B1 antigen characteristically expressed on extracellular matrices of human malignant tumors is a large chondroitin sulfate proteoglycan,PG-M/versican." Cancer Res. 56. 3902-3908 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yada, T.et al.: "Transient accumulation of perisinusoidal chondroitin sulfate proteoglycans during liver regeneration and development." J Histochem Cytochem. 44.9. 969-980 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mataga, T.et al.: "Establishment of a novel chondrocyte-like cell line derived from transgenic mice harboring the temperature-sensitive simian virus 40 large t-antigen gene." J Bone Miner Res. 11.11. 1646-1654 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kurita, K.et al.: "Occurrence of PG-Lb,a leucine-rich small chondroitin/dermatan sulaphate proteoglycan in mammalian epiphyseal cartilage:molecular cloning and sequence analysis of the mouse cDNA." Biochem J. 318. 909-914 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高木 秀和、木全 弘治: "1-3 細胞基質間接着におけるプロテオグリカンの役割" 実験医学. 14.17. 22-28 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sawai, T., Usui, N.et al.: "Hyaluronic acid of wound fluid in adult and fetal rabbits." J Pediatr Surg. 32・1. 41-43 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Isogai, Z., Yoneda, M.et al.: "Altered expression hyaluronan-richi matrices composed of PG-M/versican,SHAP and hyaluronan in solar elastosis." Nagoya Med J. 41・1. 27-37 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Specer, A.P.et al.: "Chromosomal localization of the human and mouse hyaluronan synthase genes." Genomics. 41. 493-497 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Karasawa, K.et al.: "Inhibition of experimental metastasis and cell adhesion of murine melanoma cells by chondroitin sulfate-derivatized lipid,a neoproteoglycan with anti-cell adhesion activity." Clin Exp Metastasis. 15. 83-93 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zako, M.et al.: "Alternative splicing of the unique “PLUS"domain of chiken PG-M/versican is developmentally regulated." J Biol Chem. 272・14. 9325-9331 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhao, M., Yoneda, M.et al.: "Binding of inter-α-trypsin inhibitor (ITI)to PG-M/versican and its role in the formation of hyaluronan-rich matrix." CD-ROM Proceed.3rd Internet World Cong.Biomed.Sci.98 Riken.SAQ0117-AH0108 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zako, M., Shinomura, T.et al.: "Transient expression of PG-M/versican,a large chondroitin sulfate proteoglycan in developing chicken retina." J Neurochemistry. 69・5. 2155-2161 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyama-Sorimachi, N.et al.: "Widespread expression of chondroitin sulfate-type serglycins with CD44 binding ability in hematopoietic cells." J Biol Chem. 272・42. 26714-26719 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ming, Z., Yoneda, M.et al.: "Inter α trypsin inhibitor and extracellular matrix." Progress in Biochemistry and Biophysics. 24・3. 220-223 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 栗田 和洋、木全 弘治: "抗-接着プロテオグリカンと細胞分化" 組織培養工学. 23・6. 10-14 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木全 弘治、神奈木 怜児: "細胞接着分子・細胞外マトリックス-解明される細胞認識・シグナル伝達の分子機能-" 細胞工学. 16. 786-790 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高木 秀和、木全 弘治: "II.細胞外マトリックスとの接着-3 抗-細胞接着活性をもつ細胞外マトリックス" 細胞工学. 16・6. 850-856 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 板野 直樹、木全 弘治: "4 転移における細胞外マトリックスの役割" BIO Clinica. 12・9. 601-606 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 吉田 衛、木全 弘治: "軟骨マトリックスの構造と機能 プロテオグリカン、コラーゲン研究の最近の進歩" THE BONE. 11・3. 65-79 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 杉浦 信夫、木全 弘治: "GAG-脂質誘導体を用いた細胞接着阻害実験" 細胞工学別冊 実験プロトコールシリーズ グライコバイオロジー実験プロトコール-糖タンパク質・糖脂質・プロテオグリカン- 秀潤社, (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Perris, R., Perissinotto, D., Pettway, Z., et al.: "Inhibitory effects of PG-H/aggrecan and PG-M/versican on avian neural crest cell migration." The FASEB J. 10. 293-301 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takanosu, M., Amasaki, H., Matsumoto, S., et al.: "Distribution of chondroitin sulphate proteoglycans and peanut agglutinin-binding molecules during bovine fetal palatine ridge formation." J Anat. 189. 109-115 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Isogai, Z., Shinomura, T., Yamakawa, N., et al.: "2B1 antigen characteristically expressed on extracellular matrices of human malignant tumors is a large chondroitin sulfate proteoglycan, PG-M/versican." Cancer Res. 56. 3902-3908 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yada, T., Koide, N., Kimata, K.: "Transient accumulation of perisinusoidal chondroitin sulfate proteoglycans during liver regeneration and development." J Histochem Cytochem. 44. 969-980 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mataga, N., Tamura, M., Yanai, N., et al.: "Establishment of a novel chondrocyte-like cell line derived from transgenic mice harboring the temperature-sensitive simian virus 40 large t-antigen gene." J Bone Miner Res. 11. 1646-1654 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kurita, K., Shinomura, T., Ujita, M., et al.: "Occurrence of PG-Lb, a leucine-rich small chondroitin/dermatan sulphate proteoglycan in mammalian epiphyseal cartilage : molecular cloning and sequence analysis of the mouse cDNA." Biochem J. 318. 909-914 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sawai, T., Usui, N., Sando, K., et al.: "Hyaluronic acid of wound fluid in adult and fetal rabbits." J Pediatr Surg. 32. 41-43 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Isogai, Z., Yoneda, M., Itano, N., et al.: "Altered expression hyaluronan-rich matrices composed of PG-M/versican, SHAP and hyaluronan in solar elastosis." Nagoya Med J. 41. 27-37 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Spicer, A.P., Seldin, M.F., Olsen, A s., et al.: "Chromosomal localization of the human and mouse hyaluronan synthase genes." Genomics. 41. 493-497 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Karasawa, K., Sugiura, N., Hori, Y., et al.: "Inhibition of experimental metastasis and cell adhesion of murine melanoma cells by chondroitin sulfate-derivatized lipid, a neoproteoglycan with anti-cell adhesion activity." Clin Exp Metastasis. 15. 83-93 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zako, M., Shinomura, T., Kimata, K.: "Alternative splicing of the unique "PLUS" domain of chiken PG-M/versican is developmentally regulated." J Biol Chem. 272. 9325-9331 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhao, M., Yoneda, M., Huang, L., et al.: "Binding of inter-a-trypsin inhibitor (ITI) to PG-M/versican and its role in the formation of hyaluronan-rich matrix." CD-ROM Proceed.3rd Internet World Cong.Biomed.Sci.96 Riken.SAQ0117-AH0108 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zako, M., Shinomura, T., Miyaishi, O., et al.: "Transient expression of PG-M/versican, a large chondroitin sulfate proteoglycan in developing chicken retina." J Neurochemistry. 69. 2155-2161 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyama-Sorimachi, N., Kitamura, F., et al.: "Widespread expression of chondroitin sulfate-type serglycins with CD44 binding ability in hematopoietic cells." J Biol Chem. 272. 26714-26719 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ming, Z., Yoneda, M., Kimata, K.: "Inter alpha trypsin inhibitor and extracellular matrix." Progress in Biochemistry and Biophysics. 24. 220-223 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Toyama-Sorimachi,et al.: "Widespread expression of chondroitin sulfate-type serglycins with CD44 binding ability in hematopoietic cells." J Biol Chem. 272・42. 26714-26719 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Zako, T.Shinomura,et al.: "Transient expression of PG-M/versican,a large chondroitin sulfate proteoglycan in developing chicken retina." J Neurochemistry. 2155-2161 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Zhao, M.Yoneda,et al.: "Binding of inter-a-trypsin inhibitor(ITI)to PG-M/versican and its role in the formation of hyaluronan-rich matrix." CD-ROM Proceed.3rd Internet World Cong.Biomed.Sci.96 Riken.69・5. SAQ0117-AH0108 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Zako,et al.: "Alternative splicing of the unique"PLUS"domain of chiken PG-M/versican is developmentally regulated." J Biol Chem. 272・14. 9325-9331 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Karasawa,et al.: "Inhibition of experimental metastasis and cell adhesion of murine melanoma cells by chondroitin sulfate-derivatized lipid,a neoproteoglycan with anti-cell adhesion activity." Clin Exp Metastasis. 15. 83-93 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Yoneda, Z.Ming,et al.: "Inter α trypsin inhibitor and extracellular matrix." Progress in Biochemistry and Biophysics. 24・3. 220-223 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.P.Spicer,et al.: "Chromosomal localization of the human and mouse hyaluronan synthase genes." Genomics. 41. 493-497 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Z.Isogai, M.Yoneda,et al.: "Altered expression hyaluronan-rich matrices composed of PG-M/versican,SHAP and hyaluronan in solar elastosis." Nagoya Med J. 41・1. 27-37 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Sawai, N.Usui,et al.: "Hyaluronic acid of wound fluid in adult and fetal rabbits." J Pediatr Surg. 32・1. 41-43 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木全弘治、 神奈木玲児: "細胞接着分子・細胞外マトリックス-解明される細胞認識・シグナル伝達の分子機能-" 細胞工学. 16・6. 786-790 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高木秀和、 木全弘治: "II.細胞外マトリックスとの接着-3 抗-細胞接着活性をもつ細胞外マトリックス" 細胞工学. 16・6. 850-856 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 栗田和洋、 木全弘治: "抗-接着プロテオグリカンと細胞分化" 組織培養工学. 23・6. 10-14 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉田衛、 木全弘治: "軟骨マトリックスの構造と機能 プロテオグリカン、コラーゲン研究の最近の進歩" THE BONE. 11・3. 65-79 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 板野直樹、 木全弘治: "4 転移における細胞外マトリックスの役割" BIO Clinica. 12・9. 601-606 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Perris,R.et al.: "Inhibitory effects of PG-H/aggrecan and PG-M/versican on avian neural crest cell migration" FASEB J. 10・2. 293-301 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takanosu,M.et al.: "Distribution of chondroitin sulphate proteoglycans and peanut agglutinin-binding molecules during bovine fetal palatine ridge formation." J Anat. 189. 109-115 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Isogai,Z.et al.: "2B1 antigen characteristically expressed on extracellular matrices of human malignant tumors is a large chondroitin sulfate proteoglycan, PG-M/versican." Cancer Res. 56. 3902-3908 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yada,T.et al.: "Transient accumulation of perisinusoidal chondroitin sulfate proteoglycans during liver regeneration and development." J Histochem Cytochem. 44・9. 969-980 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mataga,N.et al.: "Establishment of a novel chondrocyte-like cell line derived from transgenic mice harboring the temperature-sensitive simian virus 40 large t-antigen gene." J Bone Miner Res. 11・11. 1646-1654 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kurita,K.et al.: "Occurrence of PG-Lb, a leucine-rich small chondroitin/dermatan sulphate proteoglycan in mammalian epiphyseal cartilage : molecular cloning and sequence analysis of the mouse cDNA." Biochem J. 318. 909-914 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高木秀和、木全弘治: "細胞基質間接着におけるプロテオグリカンの役割" 実験医学. 14・17. 22-28 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 杉浦信夫、木全弘治: "GAG-脂質誘導体を用いた細胞接着阻害実験" 細胞工学別冊 実験プロトコールシリーズ グライコバイオロジー実験プロトコール-糖タンパク質・糖脂質・プロテオグリカン-秀潤社, (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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