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Analysis of the mechanism of eukaryotic transcription initiation and its regulation based on the TATA box-binding factor TFIID

Research Project

Project/Area Number 08458191
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo, Institute of Molecular and Cellular Biosciences

Principal Investigator

HORIKOSHI Masami  The University of Tokyo, Institute of Molecular and Cellular Biosciences, Associate Professor, 分子細胞生物学研究所, 助教授 (70242089)

Project Period (FY) 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1996: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsTATA-box binding factor / TFIID / TBP / Transcription regulation / Transcription activation / Chromatin transcription / Transcriptional regulatory factor / Interaction between DNA and transcription factors
Research Abstract

TATA box-binding factor TFIID plays a crucial role in machanisms of transcription activation.
We elucidated the significance of function and structure of TFIID in eukaryotic transcription initiation and its regulation by several methods.
1)Isolation and characterization of cDNA encoding human TFIID subunits
Two novel types of cDNAs, which encode human TFIID subunits and have similalities to histones, were isolated. The analysis of interactions between these subunits and other TFIID subunits has been made.
2)Model of histone octamer-like structure within TFIID
We isolated TFIID subunit whose structure is similar to that of histone H2B using other TFIID subunit with histone H4 similarity as a probe and showed that the interaction of two subunits is caused by their own histone folding motifs. We also isolated other TFIID subunit similar to histone H2A and showed a model of histone octomer-like structure.
3)Isolation of cDNA encoding TFIID subunits from several species
We isolated cDNA encoding TFIID subunits from several species in order to elucidate the relationship between structure and function.
4)Genetical analysis of S.pombe TFIID subunits
To elucidate the in vivo function of TFIID subunits, we isolated S.pombe TFIID subunits. New method for isolation of temparature sensitive mutants are developed. Isolated ts mutant TFIID subunit shows defect in cytokinesis.
5)Isolation of cDNAs encoding various new factors interacting with TFIID subunits
cDNAs encoding various new factors interacting with TFIID subunits were isolated. Most of these isolated factors are chromatin-related. Some of them are shown to have histone acetyl transferase activities and nucleosome-remodeling activities.

Report

(2 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] T.Kuzuhara & M.Horikoshi: "Isolation and characterization of a cDNA encoding a human TFIID subunit containing a variety of putative structural motifs including direct repeats" Biol.Pharm.Bull. 19. 122-126 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] B.Choi, M.Bando, S.Hasegawa & M.Horikoshi: "Isolation and characterization of a cDNA encoding a novel human transcription factor TFIID subunit containing similarities with histones H2B and H3" Gene. 169. 263-267 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Hasegawa, B.Choi & M.Horikoshi: "Isolation of Xenopus laevis TFIID subunit p22 reveals two distinct structural regions" Gene. 169. 285-286 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yamamoto & M.Horikoshi: "Cloning of the cDNA encoding a novel subtype of histone H1" Gene. 173. 281-285 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Nishikawa, T.Kokubo, M.Horikoshi, R.G.Roeder & Y.Nakatani: "Drosophila TAF_<II>230 and the transciptional activator VP16 bind competitively to the TATA box-binding domain of TBP" Proc.Natl.Acad.Sci.U.S.A. 94. 85-90 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Tao, M.Guermah, E.Martinez, T.Oelgeschlager, S.Hasegawa, R.Takada, T.Yamamoto, M.Horikoshi & R.G.Roeder: "Specific interactions and potential functions of human TAF_<II>100" J.Biol.Chem.272. 6714-6721 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Bando, S.Ijuin, S.Hasegawa & M.Horikoshi: "Involvement of the histone fold motifs in the mutual interaction between human TAF_<II>80 and TAF_<II>22 subunits of TATA box-binding factor TFIID" J.Biochem.121. 591-597 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Umehara, S.Kida, S.Hasegawa, H.Fujimoto & M.Horikoshi: "Restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis" J.Biochem.121. 598-603 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yamamoto, D.Poon, P.A.Weil & M.Horikoshi: "Molecular genetic elucidation of the tripartite structure of the Schizosaccharomyces pombe 72kDa TFIIDsubunit which contains a WD40 structural motif" Genes Cells. 2. 245-254 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yamamoto & M.Horikoshi: "Novel substrate specificity of the histone acetyltransferase activity of HIV-1-Tat interactive protein Tip60" J.Biol.Chem.272. 30595-30598 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Kuzuhara & M.Horikoshi: "Isolation and characterization of a cDNA encoding a human TFIID subunit containing a variety of putative structural motifs including direct repeats" Biol.Pharm.Bull.19. 122-126 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] B.Choi, M.Bando, S.Hasegawa & M.Horikoshi: "Isolation and characterization of a cDNA encoding a novel human transcription factor TFIID subunit containing similarities with histones H2B and H3" Gene. 169. 263-267 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Hasegawa, B.Choi & M.Horikoshi: "Isolation of Xenopus laevis TFIID subunit p22 reveals two distinct structural regions" Gene. 169. 285-286 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yamamoto & M.Horikoshi: "Cloning of the cDNA encoding a novel subtype of histone H1" Gene. 173. 281-285 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Nishikawa, T.Kokubo, M.Horikoshi, R.G.Roeder & Y.Nakatani: "Drosophila TAF_<II>230 and the transcriptional activator VP16 bind competitively to the TATA box-binding domain of the TATA box-binding protein" Proc.Natl.Acad.Sci.U.S.A.94. 85-90 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Tao, M.Guermah, E.Martinez, T.Oelgeschlager, S.Hasegawa, R.Takada, T.Yamamoto, M.Horikoshi & R.G.Roeder: "Specific interactions and potential functions of human TAF_<II>100" J.Biol.Chem.272. 6714-6721 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Bando, S.Ijuin, S.Hasegawa & M.Horikoshi: "The involvement of the histone fold motifs in the mutual interaction between human TAF_<II>80 and TAF_<II>22" J.Biochem.121. 591-597 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Umehara, S.Kida, S.Hasegawa, H.Fujimoto & M.Horikoshi: "Restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis" J.Biochem.121. 598-603 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yamamoto, D.Poon, P.A.Weil & M.Horikoshi: "Molecular genetic elucidation of the tripartite structure of the Schizosaccharomyces pombe 72 kDa TFIID subunit which contains a WD40 structural motif" Genes Cells. 2. 245-254 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yamamoto & M.Horikoshi: "Novel substrate specificity of the histone acetyltransferase activity of HIV-1-Tat interactive protein Tip60" J.Biol.Chem.272. 30595-30598 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Kuzuhara & M.Horikoshi: "Isolation and characterization of a cDNA encoding a human TFIID subunit containing a variety of putative structural motifs including direct repeats" Biol.Pharm.Bull.19. 122-126 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] B.Choi,M.Bando,S.Hasegawa & M.Horikoshi: "Isolation and characterization of a cDNA encoding a novel human transcription factor TFIID subunit containing similarities with histones H2B and H3" Gene. 169. 263-267 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Hasegawa,B.Choi & M.Horikoshi: "Isolation of Xenopus laevis TFIID subunit p22 reveals two distinct structural regions" Gene. 169. 285-286 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Yamamoto & M.Horikoshi: "Cloning of the cDNA encoding a novel subtype of histone H1" Gene. 173. 281-285 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] J.Nishikawa,T.Kokubo,M.Horikoshi,R.G.Roeder & Y.Nakatani: "Drosophila TAF_<II>230 and the transciptional activator VP16 bind competitively to the TATA box-binding domain of TBP" Proc.Natl.Acad.Sci.U.S.A.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Bando,S.Ijuin,S.Hasegawa & M.Horikoshi: "Involvement of the histone fold motifs in the mutual interaction between human TAF_<II>80 and TAF_<II>22 subunits of TATA box-binding factor TFIID" J.Biochem.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Umehara,S.Kida,S.Hasegawa,H.Fujimoto & M.Horikoshi: "Restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis" J.Biochem.(in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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