| Project/Area Number |
08458192
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NATORI Shunji The University of Tokyo, Faculty of Pharmaceutical Sciences, Professor, 大学院・薬学系研究科, 教授 (50012662)
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| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | antibacterial peptide / insect / neutrophil / superoxide / receptor / pertussis toxin / GTP-binding protein / バクテリア / サ-ベシンB |
|---|
| Research Abstract |
An antibacterial peptide, KLKLLLLLKLK-NH_2 (L5) showed significant chemotherapeutic activity in MRSA-infected mice in vivo. We found that L5 activated the human neutrophil, resulting the production of superoxide. The binding protein with a molecular mass of 55 kDa was purified from membrane proteins of human neutrophil using affinity column chromatography conjugated with L5. The pertussis toxzin inhibited the activation of L5 to the neutrophil, suggesting that the signal of L5 into the neutrophil is mediated via GTP-binding protein. The binding protein with a molecular mass of 55 kDa is expected to be the target molecule to search for effective drugs for some infectious diseases.
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