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Analysis of the receptor of an antibacterial peptide on human neutrophils.

Research Project

Project/Area Number 08458192
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

NATORI Shunji  The University of Tokyo, Faculty of Pharmaceutical Sciences, Professor, 大学院・薬学系研究科, 教授 (50012662)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywordsantibacterial peptide / insect / neutrophil / superoxide / receptor / pertussis toxin / GTP-binding protein / バクテリア / サ-ベシンB
Research Abstract

An antibacterial peptide, KLKLLLLLKLK-NH_2 (L5) showed significant chemotherapeutic activity in MRSA-infected mice in vivo. We found that L5 activated the human neutrophil, resulting the production of superoxide. The binding protein with a molecular mass of 55 kDa was purified from membrane proteins of human neutrophil using affinity column chromatography conjugated with L5. The pertussis toxzin inhibited the activation of L5 to the neutrophil, suggesting that the signal of L5 into the neutrophil is mediated via GTP-binding protein. The binding protein with a molecular mass of 55 kDa is expected to be the target molecule to search for effective drugs for some infectious diseases.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] Yuki, nakajima: "Chimotherapeutic activity of synthetic antimicrobial peptides:correlation between chemotherapeutic activity and neutrophil-activating activity" FEBS Letters. 415. 64-66 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuki, Nakajima: "A Novel Protease in the Pupal Yellow Body of Sarcophaga pergrina(Flesh Fly)" J.Biol.Chem.272(38). 23805-23810 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makoto, Shimoaraiso: "Identification of the Region in Yeast S-II that Defines Species Specificity in its Interaction with RNA Polymerase II" J.Biol.Chem.272(42). 26550-26554 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shihei, Hori.: "Monoclonal Antibodies against Pupa-Specific Surface Antigens of Sarcophaga perehrina(Flesh Fly)Hemocytes" B.B.R.C.236. 497-501 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mariko, Hijikata.: "Inhibition of Protein Tyrosine Kinase by 5-S-GAD,a Novel Antibacterial Substance from an Insect" B.B.R.C.237. 423-426 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takekazu, Kunieda: "Regeneration of Sarcophaga Imaginal Discs in Vitro:Implication of 20-Hydroxyecdysone" Developmental Biology. 183. 86-94 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shunji, Natori: "Molecular Mechanisms of Immune Responses in Insects" Chapman&Hall, 325 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeo Kubo, Masami Sasaki, Jun Nakamura, Hitomi Sasagawa, Kazuaki Ohashi, Hideaki Takeuchi and Shunji Natori: "Change in the expression of hypophryngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role" J.Biochem.119. 291-295 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiromi Sugiyama, and Shunji Natori: "Transmethylation reaction is essential for Sarcophaga lectin gene activation" J.Biochem.119. 354-359 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuaki Ohashi, Miyuki Sawata, Hideaki Takeuchi, Shunji Natori and Takeo Kubo: "Molecular cloning of cDNA and analysis of expression of the gene for alpha-glucosidase from the hypophryngeal gland of the honeybee (Apis mellifera L.)" Biochem.Biophys.Res.Commun.221. 380-385 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahiro Ito, Qihong Xu, Hideaki Takeuchi, Takeo Kubo and Shunji Natori: "Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II" FEBS Lett.385. 21-24 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kiyoshi Kawasaki, Takeo Kubo and Shunji Natori: "Presence of the Periplaneta lectin-related protein family in the American cockroach (Periplaneta americana)" Insect Biochem.Molec.Biol.26. 355-364 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ko-ichi Homma, Takefumi Matsushita and Shunji Natori: "Purification, characterization, and CDNA cloning of a novel growth factor from the conditioned medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina (flesh fly)" J.Biol.Chem.271. 13770-13775 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jae Yoon Leem, Chiaki Nishimura, Shoichiro Kurata, Ichio Shimada, Ayako Kobayashi and Shunji Natori: "Purification and characterization of N-beta-Alanyl-5-S-qlutathionyl-3,4-dihydroxyphenylalanine, a novel antibacterial substance of Sarcophgaperegrina (flesh fly)" J.Biol.Chem.271. 13572-13577 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomohiro Horio, Takeo Kubo and Shunji Natori: "Purification and cDNA cloning of the alcohol dehydrogenase of the flesh fly Sarcophaga peregrina A structural relationship between alcohol dehydrogenase and a 25-kDa protein" Eur.J.Biochem.237. 698-703 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shafiqal Huq, Takeo Kubo, Shoichiro Kurata, Ayako Kobayashi and Shunji Natori: "Purification, characterization, and cDNA cloning of a galactose-specific c-type lectin from Drosophila melanogaster" J.Biol.Chem.271. 20213-20218 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshiyuki Sekizawa, Naoshi Ohta, Shunji Natori and Hideshi Kobayashi: "Immunocytochemical localization of lysenin, a novel protein isolated from the coelomic fluid of the earthworm Eisenia foetida" Biomedical Research. 17. 327-330 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ko-ichi Homma and Shunji Natori: "Identification of substrate proteins for cathepsin L that are selectively hydrolyzed during the differentiation of imaginal discs of Sarcophaga peregrina" Eur.J.Biochem.240. 443-447 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeo Kubo, Toshimitsu Arai, Kiyoshi Kawasaki and Shunji Natori: "Insect lectins and epimorphosis" Trends in glycoscience and glycotechnology. 8. 357-364 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nobuaki Adachi, Takeo Kubo and Shunji Natori: "Purification, characterization, and cDNA cloning of ABP-2 (arylphorin gene-specific binding protein-2) that specifically binds to the ABP-1-binding sequence in the arylphorin gene of Sarcophaga peregrina" J.Biochem.120. 1239-1246 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yutaka Hirakura, Juan Alvarez-Bravo, Shoichiro Kurata, Shunji Natori and Yutaka Kirino: "Selective interaction of synthetic antimicrobial peptides derived from sapecin B with lipid bilayrs" J.Biochem.120. 1130-1140 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takekazu Kunieda, Shoichiro Kurata and Shunji Natori: "Regeneration of Sarcophaga imaginal discs in vitro : Implication of 20-hydroxyecdysone" Dev.Biol.183. 86-94 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shunji Natori and Takeo Kubo: "Role of lectins in development and morphogen in insects" New Direction in Invertebrate Immunology. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sumio Ohtsuki, Ko-ichi Homma, Shoichiro Kurata and Shunji Natori: "Molecular cloning of cDNA for Sarcophaga prolyl endopeptidase and characterization of the recombinant enzyme produced by an E.coli expression system" Insect Biochem.Molec.Biol.27. 337-343 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sumio Ohtsuki, Ko-ichi Homma, Shoichiro Kurara and Shunji Natori: "Nuclear localization and involvement in DNA synthesis of Sarcophaga prolyl endopeptidase" J.Biochem.121. 1176-1181 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamaki Yano, Ayako Kobayashi, Shoichiro Kurata and Shunji Natori: "Purification and characterisation of cathepsin B mRNA 3'-untranslated-region-binding protein (CBBP), a protein that represses cathepsin B mRNA translation" Eur.J.Biochem.245. 260-265 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shohei Hori, Ayako Kobayashi and Shunji Natori: "Monoclonal antibodies against pupa-specific surface antigens of Sarcophaga peregrina (flesh fly) hemocytes" Biochem.Biophs.Res.Commun.236. 497-501 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuki Nakajima, Yumiko Tsuji, Ko-ichi Homma and Shunji Natori: "A novel protease in the pupal yellow body of Sarcophaga peregrina (flesh fly)" J.Biol.Chem.272. 23805-23810 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mariko Hijikata, Ayako Kobayashi, Jae Yoon Leem, Hidesuke Fukasawa, Yoshimasa Uehara and Shunji Natori: "Inhibition of protein tyrosine kinase by 5-S-GAD,a novel antibacterial substance from an insect" Biochem.Biophs.Res.Commun.237. 423-426 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuki Nakajima, Juan Alvarez-Bravo, Jang-Hyun Cho, Ko-ichi Homma, Shiro Kanegasaki and Shunji Natori: "Chemotherapeutic activity of synthetic antimicrobial peptides : Correlation between chemotherapeutic acitvity and neutrophil-activating activity" FEBS Lett.415. 64-66 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makoto Shimoaraiso, Toshiyuki Nakanishi, Takeo Kubo and Shunji Natori: "Identification of the region in yeast S-II that defines species specificity in its interaction with RNA polymerase II" J.Biol.Chem.272. 26550-26554 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshiyuki Sekizawa, Takeo Kubo, Hideshi Kobayashi, Terumi Nakajima and Shunji Natori: "Molecular cloning of cDNA for lysenin, a novel protein in the earthworm Eisenia foetida that causes contraction of rat vascular smooth muscle" Gene. 191. 79-102 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuaki Ohashi, Shunji Natori and Takeo Kubo: "Change in the mode of gene expression of the hypopharyngeal galnd cells with an age-dependent role change of the worker honeybee Apis millifera L." Eur.J.Biochem.249. 797-802 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Azusa Kamikouchi, Hideaki Takeuchi, Miyuki Sawata, Kazuaki Ohashi, Shunji Natori and Takeo Kubo: "Preferential expression of the gene for a putative inositol 1,4,5-trisphosphate receptor homologue in the mushroom bodies of the brains of the worker honeybee Apis mellifera L." Biochem.Biophs.Res.commun.242. 181-186 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaji Okamoto, Ichiro Mitsuhara, Masahiro Ohshima, Shunji Natori and Yuko Ohashi: "Enhanced expression of an antimicrobial peptide Sarcotoxin IA by GUS Fusion in Transgenic Tobacco Plants" Plant Cell.Physiol.39. 57-63 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuki Nakajima: "Chemotherapeutic activity of synthetic antimicrobial peptides : correlation between chemotherapeutic activity and neutrophil-activating activity" FEBS Letters. 415. 64-66 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yuki Nakajima: "A Novel Protease in the Pupal Yellow Body of Sarcophaga peregrina (Flesh Fly)" J.Biol.Chem.272(38). 23805-23810 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Makoto Shimoaraiso: "Identification of the Region in Yeast S-II that Defines Species Specificity in its Interaction with RNA Polymerase II" J.Biol.Chem.272(42). 26550-26554 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shohei Hori: "Monoclonal Antibodies against Pupa-Specific Surface Aantigens of Sarcophaga peregrina (Flesh Fly) Hemocytes" B.B.R.C.236. 497-501 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mariko Hijikata: "Inhibition of Protein Tyrosine Kinase by 5-S-GAD, a Novel Antibacterial Substance from an Insect" B.B.R.C.237. 423-426 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takekazu Kunieda: "Regeneration of Sarcophaga Imaginal Discs in Vitro : Implication of 20-Hydroxyecdysone" Developmental Biology. 183. 86-94 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shunji Natori: "Molecular Mechanisms of Immune Responses in Insects" Chapman & Hall, 325 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ko-ichi Homma: "Purification,characterization,and cDNA cloning of a novel growth factor from the conditioned medium of NIH-sape-4,an embryonic cell line of Sarcophaga peregrina(flesh fly)" J.Biol.Chem.271. 13770-13775 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jae Yoon Leem: "Purification and characterization of N-β-alany-5-S-glutathionyl-3,4-dihydroxyphenylalanine,a novel antibacterial substance of Sarcophaga peregrina(flesh fly)" J.Biol.Chem.271. 13573-13577 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tomohiro Horio: "Purification and cDNA cloning of the alcohol dehydrogenase of the flesh fly Sarcophage peregrina a structural relationship between alcohol dehydrogenase and a 25-kDa protein" Eur.J.Biochem.237. 698-703 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshiyuki Sekizawa: "Immunocytochemical localization of lysenin,a novel protein isolated from the coelomic fluid of the earthworm Eisenia foetida" Biomed.Res.17. 327-330 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kiyoshi Kawasaki: "Presence of the periplaneta lectin-related protein family in the american cockroach Periplaneta americana" Insect Biochem.Molec.Biol.26. 355-364 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takahiro Ito: "Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II." FEBS Letters. 385. 21-24 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shunji Natori: "New Directions in Invertebrate Immunology" SOS Publications, 494 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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