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Mechanism of Quinonoid Cofactor Formation in Copper Amine Oxidase and Catalytic Mechanism Involving Radical Intermediates

Research Project

Project/Area Number 08458196
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionOsaka University

Principal Investigator

TANIZAWA Katsuyuki  Osaka University, Institute of Scientific and Industrial Research, Professor, 産業科学研究所, 教授 (20133134)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1996: ¥5,200,000 (Direct Cost: ¥5,200,000)
Keywordsquinonoid cofactor / copper amine oxidase / post-translational modification / topa quinone / X-ray crystallographic structure / site-directed mutagenesis / 翻訳後修飾機構 / ラジカル触媒
Research Abstract

Copper amine oxidase catalyzes oxidative deamination of various biogenic amines, occurring widely in microorganisms, plants and animals. Besides copper, the enzyme contains a recently identified quinonoid cofactor, 2,4,5-trihydroxyphenylalanyl quinone (TPQ), covalently bound to the protein. In this study, the genes coding for phenylethylamine oxidase and histamine oxidase have been cloned from a Coryne-form bacterium Arthrobacter globiformis and sequenced. Using the recombinant apoenzyme overproduced in E.coil cells, the TPQ cofactor has been demonstrated to be produced from a specific tyrosyl residue by self-processing of the protein upon binding divalent copper ions. The process of TPQ formation has been investigated in detail by UV-VIS,CD,ESR,and resonance Raman spectroscopies to show that the cupric ion bound to the apoenzyme is first reduced to a cuprous ion, that the cuprous ion likely activates dioxygen for oxidative modification of the precursor tyrosyl residue, that a semiquin … More one radical intermediate is formed during the TPQ formation, and that the carbonyl C-2 oxygen of TPQ is derived from solvent. To elucidate the role(s) of the conserved sequence Ans-Tyr-Asp/Glu-Tyr in the biogenesis of TPQ,each of the corresponding residues at positions 401-404 in the recombinant histamine oxidase has been replaced with other amino acids by site-directed mutagenesis. When Asn-401 was changed to Asp or Gln, the rate of TPQ formation by copper-dependent self-processing was 10^3-to 10^4-fold slower than in the wild-type enzyme. When Tyr-402 was replaced by Phe, TPQ was not formed at all, showing that Tyr-402 is essential as the precursor to TPQ.In contrast, Asp-403 could be replaced by Glu without changes in the rate of TPQ formation, whereas its replacement by Asn led to a marked decrease. These results collectively indicate that a very rigorous structural motif is required for efficient formation of TPQ and for the catalytic activity in the active site of copper amine oxidases. The crystal structures phenylethylamine oxidase have been determined and refined for the inactive apo and active holo forms both at 2.2 resolution. Significant differences between the two forms are limited to the active site (positions and orientations of the precursor Tyr and TPQ and a His residue binding copper, suggesting that there occurrs no large conformational changes of the protein during the TPQ biogenesis. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] N.Nakamura, R.Matsuzaki, Y.-H.Choi, K.Tanizawa, and J.Sanders-Loehr: "Biosynthesis of Topa Quinone Cofactor in Bacterial Amine Oxidases. Solvent Origin of C-2 Oxygen Determined by Raman Spectroscopy." J.Biol.Chem.271・9. 4718-4724 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.-H.Choi, R.Matsuzaki, S.Suzuki, and K.Tanizawa: "Role of Conserved Asn-Tyr-Asp-Tyr Sequence in Bacterial Copper/2,4,5-Trihydroxyphenylalanyl Quinone-Containing Histamine Oxidase." J.Biol.Chem.271・37. 22598-22603 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Mure and K.Tanizawa: "Chemical and Biochemical Characteristics of Topa Quinone." Biosci.Biotech.Biochem.61・3. 410-417 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Shimizu, K.Ohta, S.Takayama, Y.Kitagaki, K.Tanizawa, and T.Yorifuji: "Purification and Properties of Phenylethylamine Oxidase of Arthrobacter globiformis." Biosci.Biotech.Biochem.61・3. 501-505 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.E.Ruggiero, J.A.Smith, K.Tanizawa, and D.M.Dooley: "Mechanistic Studies of Topa Quinone Biogenesis in Phenylethylamine Oxidase." Biochemistry. 36・8. 1953-1959 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 谷澤 克行: "銅アミンオキシダーゼにおけるトパキノン補酵素生成のしくみ" 化学と生物. 35・8. 569-575 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Nakamura, P.Moenne-Loccoz, K.Tanizawa, M.Mure, S.Suzuki, J.P.Klinman, and J.Sanders-Loehr: "Topaquinone-Dependent Amine Oxidases. Identification of Reaction Intermediates by Raman Spectroscopy." Biochemistry. 36・38. 11479-11486 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.C.J.Wilce, D.M.Dooley, H.C.Freeman, J.M.Guss, H.Matsunami, W.S.Mclntire, C.E.Ruggiero, K.Tanizawa, and H.Yamaguchi: "Crystal Structures of the Copper-Containing Amine Oxidase from Arthrobacter globiformis in the Holo-and Apo-Forms : Implications for the Biogenesis of Topa Quinone." Biochemistry. 36・51. 16116-16133 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nobufumi NAKAMURA,Ryuichi MATSUZAKI,Yoon-Ho CHOI,Katsuyuki TANIZAWA,and Joann SANDERS-LOEHR: "Biosynthesis of Topa Quinone Cofactor in Bacterial Amine Oxidases. Solvent Origin of C-2 Oxygen Determined by Raman Spectroscopy" J.Biol.Chem.271-9. 4718-4724 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoon-Ho CHOI,Ryuichi MATSUZAKI,Shinnichiro SUZUKI,and Katsuyuki TANIZAWA: "Role of Conserved Asn-Tyr-Asp-Tyr Sequence in Bacterial Copper/2,4,5-Trihydroxyphenylalanyl Quinone-Containing Histamine Oxidase" J.Biol.Chem.271-37. 22598-22603 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Minae MURE and Katsuyuki TANIZAWA: "Chemical and Biochemical Characteristics of Topa Quinone" Biosci.Biotech.Biochem.61-3. 410-417 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Eiichi SHIMIZU,Keiichi OHTA,Shigeo TAKAYAMA,Yuzuru KITAGAKI,Katsuyuki TANIZAWA,and Takamitsu YORIFUJI: "Purification and Properties of Phenylethylamine Oxidase of Arthrobacter globiformis" Biosci.Biotech.Biochem.61-3. 501-505 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Christy E.RUGGIERO,Jennifer A.SMITH,Katsuyuki TANIZAWA,and David M.DOOLEY: "Mechanistic Studies of Topa Quinone Biogenesis in Phenylethylamine Oxidase" Biochemistry. 36-8. 1953-1959 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Katsuyuki TANIZAWA: "Generation Mechanism of Topa Quinone Cofactor in Copper Amine Oxidase (in Japanese)" Kagaku-to-Seibutsu. 35-8. 569-575 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nobuhumi NAKAMURA,Pierre MOENNE-LOCCOZ,Katsuyuki TANIZAWA,Minae MURE,Shinnichiro SUZUKI,Judith P.KLINMAN,and Joann SANDERS-LOEHR: "Topaquinone-Dependent Amine Oxidases. Identification of Reaction Intermediates by Raman Spectroscopy" Biochemistry. 36-38. 11479-11486 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matthew C.J.WILCE,David M.DOOLEY,Hans C.FREEMAN,J.Mitchell GUSS,Hideyuki MATSUNAMI,William S.MCINTIRE,Christy E.RUGGIERO,Katsuyuki TANIZAWA,and Hiroshi YAMAGUCHI: "Crystal Structures of the Copper-Containing Amine Oxidase from Arthrobacter globiformis in the Holo- and Apo Forms : Implications for the Biogenesis of Topaquinone" Biochemistry. 36-51. 16116-16133 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Mure and K.Tanizawa: "Chemical and Biochemical Characteristics of Topa Quinone." Biosci.Biotech.Biochem.61・3. 410-417 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.Shimizu, K.Ohta, S.Takayama, Y.Kitagaki, K.Tanizawa, and T.Yorifuji: "Purification and Properties of Phenylethylamine Oxidase of Arthrobacter globiformis." Biosci.Biotech.Biochem.61・3. 501-505 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] C.E.Ruggiero, J.A.Smith, K.Tanizawa, and D.M.Dooley: "Mechanistic Studies of Topa Quinone Biogenesis in Phenylethylamine Oxidase." Biochemistry. 36・8. 1953-1959 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷澤克行: "銅アミンオキシダーゼにおけるトパキノン補酵素生成のしくみ" 化学と生物. 35・8. 569-575 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Nakamura, P.Moenne-Loccoz, K.Tanizawa, M.Mure, S.Suzuki, J.P.Kinman, and J.Sanders-Loehr: "Topaquinone-Dependent Amine Oxidases. Identification of Reaction Intermediates by Raman Spectroscopy." Biochemistry. 36・38. 11479-11486 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.C.J.Wilce, D.M.Dooley, H.C.Freeman, J.M.Guss, H.Matsunami, W.S.McIntire, C.E.Ruggiero, K.Tanizawa, and H.Yamaguchi: "Crystal Structures of the Copper-Containing Amine Oxidase from Arthrobacter globiformis in the Holo-and Apo-Forms : Implications for the Biogenesis of Topa Quinone." Biochemistry. 36・51. 16116-16133 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Nakamura,R.Matsuzaki,Y.-H.Choi,K.Tanizawa,and J.Sanders-Loehr: "Biosynthesis of Topa Quinone Cofactor in Bacterial Amine Oxidases. Solvent Origin of C-2 Oxygen Determined by Raman Spectroscopy." J.Biol.Chem.271・9. 4718-4724 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.-H.Choi,R.Matsuzaki,S.Suzuki,and K.Tanizawa: "Role of Conserved Asn-Tyr-Asp-Tyr Sequence in Bacterial Copper/2,4,5-Trihydroxyphenylalanyl Quinono-Contaning Histamine Oxidase." J.Biol.Chem.271・37. 22598-22603 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Mure and K.Tanizawa: "Chemical and Biochemical Characteristics of Topa Quinone." Biosci.Biotech.Biochem.61・3. 410-417 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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