| Project/Area Number |
08458204
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TOYOSHIMA Yoko Univ.of Tokyo, Grad.School of Arts and Sci., Assoc.Prof., 大学院・総合文化研究化, 助教授 (40158043)
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| Co-Investigator(Kenkyū-buntansha) |
EDAMATSU Masaki Univ.of Tokyo, Grad.School of Arts and Sci., Res.Assistant, 大学院・総合文化研究化, 助手 (60251328)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | motor proteins / microtubules / kinesin / ncd / motor domain / neck region / polarity of the movement / 頸部 / ミオシン / 回転自由度 |
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| Research Abstract |
Ncd, a member of kinesin super family, moves toward the minus end of microtubule. Neck region of kineshin and ncd is considered to be important to determine the polarity of the movement, because the structural difference between the motor domains of kineshin and ncd is not essential. We made a chymeric protein which consists of kinesin motor domain and ncd neck domain. This chymeric protein did not show the directional movement on microtubules. The measurement of ATPase activity revealed that the values of Kcat and K_<50%MT> are 7.8 S^<-1> and 0.22 muM,respectively. Comparing these values with those of kineshin fragment (kineshin motor domain and kineshin neck domain), the chymeric protein seemed to be unable to perform the processive movement. These results indicate that the neck region play an important role to determine the polarity of the movement.
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