| Project/Area Number |
08458228
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
NISHIDA Yasuyoshi Nagoya University, Graduate School of Science, Professor, 大学院理学研究科, 教授 (50107059)
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| Co-Investigator(Kenkyū-buntansha) |
ADACHI-YAMADA Takashi Nagoya University, Graduate Schoool of Science, Assistant Professor, 大学院理学研究科, 助手 (20221723)
SUGIYAMA Shin Nagoya University, Graduate School of Science, Assistant Professor, 大学院理学研究科, 助手 (00270984)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1997: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1996: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | MAPK cascade / sphingolipids / actomyosine system / development / cell cycle / MAP kinase / signal transduction / Drosophila / セラミド / フォスファターゼ |
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| Research Abstract |
The MAP kinase cascade is one of the major pathways of cellular signal transduction and is involved in a wide variety of biological processes. To elucidate the networks of signal transduction responsible for the development of multi-cellular organisms, we have tried to identify novel factors which give diversity and specificity to the common MAPK cascade by the use of Drosophila molecular biology. We have screened for new P insertional mutations whose phenotypes could be either suppressed or enhanced by gain-of-function mutations for MAPKK and MAPK,and have obtained four such mutants ; Gp99, Gp126 and lace which are suppressed by dominant MAPKK mutations and Gp6 which is enhanced by them. Gp99 encodes a novel cell cycle factor with a consensus sequence for phosphorylation by MAPK and is required for the progression of M phase. We have found four hypothetical protein sequences with considerable similarities to a portion of Gp99 protein from the database of budding yeast.Destruction of two of them showed specific cell cycle defects at G1 or G2/M phases, while destruction of the remaining genes showed no obvious growth defects other than defects in meiosis. Gp6 was found to encode non-muscle myosine regulatory light chain and its high activity is required for the morphogenesis of the compound eye and the distal portion of legs. Gp126 encodes a novel protein with a short strech of sequence similar to the consensus for the catalytic site of acidic phosphatases.lace encodes serine parmitoyltransferase catalyzing the first step of sphingolipid biosynthesis. Hetero-allelic combinations of some of the alleles showed a mild phenotype associated with apoptosis, and showed genetic interactions with hep encoding MKK7 and puckered encoding a phosphatase specific to DJNK.All the mutations obtained in this study showed phenotypes different to eath other, and would be useful to elucidate how the MAPK pathway plays such a diverse biological functions.
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