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Defense Mechanism of cells from stresses

Research Project

Project/Area Number 08458235
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionThe Tokyo Metropolitan Institute of Medical Science

Principal Investigator

MATSUMOTO Seiji (1997)  RINSHOKEN,Cell Biology, Research Associate, 細胞生物学研究部門, 研究員 (40190532)

矢原 一郎 (1996)  財団法人東京都臨床医学総合研究所, 副所長 (60109957)

Co-Investigator(Kenkyū-buntansha) AIZAWA Hiroyuki  RINSHOKEN,Cell Biology, Research Associate, 細胞生物学研究部門, 研究員 (90221704)
IIDA Kazuko  RINSHOKEN,Cell Biology, Research Associate, 細胞生物学研究部門, 研究員 (40151229)
MOTIYAMA Kenji  RINSHOKEN,Cell Biology, Research Associate, 細胞生物学研究部門, 研究員 (00250217)
松本 清治  財団法人東京都臨床医学総合研究所, 細胞生物, 研究員 (40190532)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
Keywordsstress protein / heat shock protein / stress response / cell defense mechanism / HSP90 / molecular chaperone / cofilin / Actin
Research Abstract

1-1. We found taht heated HSP90 binds and protects denatured firefly luciferase from irreversible aggregation. Luciferase can be released from the complexes when post-incubated in HSP70, HSP40 and another chaperone contained in reticulocyte lysate.
1-2. We found taht monoclonal antibodies specific to the N-terminus of HSP90 bound to both ends of HSP90 dimers. This indicates that HSP90 monomers align side-by-side in an antiparallel fashion and form dimers through their C-termini.
1-3. In the budding yeast, the expression level of Hsc82 was found to be reversibly proportional to the degree of stress tolerance.
2-1. We have determined tertiary structure of destrin, a cofilin-related protein, by NMR.The structure is similar to that of a unitary segment of gelsolin. The structure provides molecula basis for Ca^<2+> -independent binding of cofilin to actin.
2-2. Overexpression of cofilin in Dictyostelium cells induced a number of actin bundles. This was suggested to be a result of transformation from actin meshwork to bundles by cofilin-induced severing of F-actin. We further demonstrated that the bundles contracted in response to osmotic stress.
2-3. We found that cofilin was inactivated by phosphorylation of the Ser-3
2-4. A multicopy suppressor to a cofilin-ts mutant in Saccharomyces cerevisiae was isolated. The suppressor gene SCF1 encodes a WD40-repeat protein which is identidal to Aip1 previously described as an actin-intereating protein. SCF1 is not essential for cell viability.
2-5. Biochemical evidence has been obtained that Dictyostelium Aip1 binds to F-actin and seisitizes it to destruction by cofiin.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Yonehara, M., et al.: "Heat-induced chaperone activity of HS90." J.Biol.Chem.271. 2641-2645 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aizawa, H.et al.: "Overexpression of cofilin stimulates bundling of actin filaments,membrane ruffling and cell movement in Dictyostelium." J.Cell Biol.132. 335-344 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hatanaka, H., et al.: "Tertiary structure of destrin and structural similarity between two actin-regulating protein families." Cell. 85. 1047-10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kimura, Y., et al.: "Cdc37 is a molecular chaperone with specific functions in signal transduction." Genes & Dev.11. 1775-1785 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aizawa, H., et al.: "Live dynamics of Dictyostelium cofilin its role in remodeling actin latticework into bundles." J.Cell Sci.110. 2333-2344 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyata, Y., et al.: "Phosphorylation of the immunesuppressant FK506-binding protein FKBP52 by casein kinase II:Regulation of HSP90-binding activigty of FKBP52." Proc.Natl.Acad.Sci.94. 14500-14505 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yonehara, M., Minami, Y., Kawata, Y., Nagai, J.and Yahara, I.: "Heat-induced chaperone activity of HSP90." J.Biol.Chem.271. 2641-2645 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aizawa, H., Sutoh, K.and Yahara, I.: "Overexpression of cofilin stimulates bundling of actin filaments, membrane fuffling and cell movement in Dictyostelium." J.Cell Biol.132. 335-344 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Moriyama, K., Iida, K.and Yahara, I.: "Phosphorylation of Ser-3 of cofilin regulates its essential function of actin." Genes to Cells. 1. 73-86 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hatanaka, H., Ogura, K., Moriyama, K., Ichikawa, S., Yahara, I.And Inagaki, F.: "Tertialry structure of destrim and structural similarity between two actin-regulating protein families." Cell. 85. 1047-1055 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Emoto, K., Kobayashi, T., Yamaji, A., Aizawa, H., Yahara, I., Inoue, K., and Umeda, M.: "Redistribution of phosphatidylethanolamine at the cleavage furrow of diving cells during cytokinesis." Proc.Natl.Acad.Sci.USA. 93. 12867-12872 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yahara, I., Aizawa, H., Moriyama, K., Iida, K., Yonezawa, H., Nishida, E., Hatanaka, N., and Ingaki, F.: "A role of cofilin/destrin in reorganization of actin cytoskeleton in response to stresses and cell stimuli." Cell Struct.Funct.21. 421-424 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aizawa, H., Sameshima, M., and Yahara, I.: "A greeen fluorescent protein-actin fusion protein dominantly inhibits cytokinasis, cell spreading, and locomotion in Dictyostelium." Cell Struct.Funct. 22. 335-345 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Csermely, P., Miyata, Y., Soti, C., and Yahara, I.: "Binding affinity of proteins to hsp90 correlates with both hydrophobicity and positive charges. A surface plasmon resonance study." Life Sciences. 61. 411-418 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kimura, Y., Rutherford, S.L., Miyata, Y., Yahara, I., Freeman, B.C., Yue, L., Morimoto, R.And Lindquist, S.: "Cdc37 is a molecular chaperone with specific functions in signal transduction." Genes & Dev.11. 1775-1785 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aizawa, H., Fukui, Y., and Yahara, I.: "Live dynamics of Dictyostelium cofilin suggests its role in remodeling actin latticework into bundles." J.Cell Sci.110. 2333-2344 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyata, Y., Chambraud, B., Radanyi, C., Leclearc, J., Lebeau, M.-G., Renoir, J.-M., Shirai, J., Catelli, M.-G., Yahara, I., and Bauliew, E.-E.: "Phosphorylation of the immunesuppressant FK506-binding protein FKBP52 by casein kinase II : Regulation of HSP90-binding activigty of FKBP52." Proc.Natl.Acad.Sci.USA. 94. 14500-14505 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aizawa,H.et al.: "A green fluorescent protein-actin fusion protein dominantly inhibits cytokinasis,cell spreading,and locomotion in Dictyostelium." Cell Struct.Funct.22. 335-345 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kimura,Y.,et al.: "Cdc37 is a molecular chaperone with specific functions in signal transduction." Genes & Dev.11. 1775-1785 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aizawa,H.,et al.: "Live dynamics of Dictyostelium cofilin suggests its role in remodeling actin latticework into bundles." J.Cell Sci.110. 2333-2344 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyata,Y.,et al.: "Phosphorylation of the immunesuppressant FK506-binding protein FKBP52 by casein kinase II:Regulation of HSP90-binding activigty of FKBP52." Proc.Natl.Acad.Sci.USA,. 94. 14500-14505 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Csermely,P.,et al.: "Binding affinity of proteins to hsp90 correlates with both hydrophobicity and positive changes. A surface plasmon resonance study." Life Sciences,. 61. 411-418 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yahara,I.,et al.: "The 90-kDa stress protein,HSP90 is a novel molecular chaperone." Ann.NY Acad.Sci.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yonehara,M.,et al.: "Heat-induced chaperone activity of HSP90." J.Biol.Chem.271. 2641-2645 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Aizawa,H.et al.: "Overexpression of cofilin stimulates bunding of actin filaments,membrane ruffling and cell movement in Dictyostelium." J.Cell Biol. 132. 335-344 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Moriyama,K.,et al.: "Phosphorylation of Ser-3 of cofilin regulates its essential function of actin." Genes to Cells.1. 73-86 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hatanaka,H.,et al.: "Tertiary structure of destrin and structural similarity between two actin-regulating protein families." Cell. 85. 1047-1055 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Emoto,K,.et al.: "Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis." Proc.Natl.Acad.Sci.USA,. 93. 12867-12872 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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