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Neuronal circuitry in relation with transmitters and their receptors

Research Project

Project/Area Number 08458245
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIZUNO Noboru  Kyoto Univ.Graduate Sch.of Med., Prof., 医学研究科, 教授 (10025596)

Co-Investigator(Kenkyū-buntansha) NOMURA Sakashi  Kyoto Univ., Coll.of Med.Technol., Prof., 医療技術短期大学部, 教授 (60034188)
OHISHI Hitoshi  Kyoto Univ., Fac.of Med., Assist.Prof., 医学部, 助手 (00252461)
TOKUNO Hironobu  Kyoto Univ., Graduate Sch.of Med., Assist.Prof., 医学研究科, 助手 (40212071)
TAKADA Masahiko  Kyoto Univ., Graduate Sch.of Med., Lecturer, 医学研究科, 講師 (00236233)
KANEKO Takeshi  Kyoto Univ., Graduate Sch, of Med., Assoc.Prof, 医学研究科, 助教授 (90177519)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥3,300,000 (Direct Cost: ¥3,300,000)
KeywordsGABA / Glycine / Glutamate receptor / Peptide / Trigeminal system / Cerebellum / Striatum / Olfactory system / セロトニン / P物質受容体 / 三叉神経核 / 痛覚 / 嗅覚 / オピオイド受容体 / エンケファリン / 免疫組織化学 / 逆行性標識法
Research Abstract

1.By combining the retrograde fluorescence-labeling method with the immunofluorescent histochemistry for glutamic acid decarboxylase or glycine, the parvicellular medullary reticular formation including the juxtatrigeminal region were found to constitute one of the main interneuron pools for inhibitory (GABAergic and/or glycinergic) premotor neurons for facial and hypoglossal motoneurons in the rat.
2.It was found immunohistochemically that mGluR2 and mGluR5 were expressed in two non-overlapping populations of Golgi cells in the cerebellum of the rat.
3.By combining immunofluorescence and fluorescene histochemical techniques with the retrograde fluorescence-labeling method, axon terminals of serotoninergic neurons were found light and eletron microscopically to be in synaptic contact with nociceptive projection neurons in the caudal spinal trigeminal nucleus of the rat ; the nociceptive projection neurons were identified by retrograde labeling with the tracers injected into contralateral … More thalamus or the ipsilateral parabrachial nucleus and by expression of FOS after subcutaneous injection of formalin into the perioral regions or by synaptic contacts with axon terminals labeled with I-B4.
4.Specific antibodies were produced against rat preprodynorphin, preproenkephalin, and preprotachykinin A.By using these antibodies, projection neurons in the striatum of the rat were divided into two groups ; the neurons of one group contained enkephalin and sent projection fibers to the globus pallidus, and those of the other group contained tachykinins and dynorphins/Leu-enkephalin and sent projection fibers to substantia nigra.
5.The distributions of mGluR7a and mGluR7b were examined immunohistochemically in the rat and mouse by using antibodies raised against rat mGluR7a and human mGluR7b. The results indicated that mGluR7 might play roles not only as an autorecepter in glutamatergic axon terminals, but also as a presynaptic heteroreceptor in non-glutamatergic axon terminals.
6.Light and electron microscopic immunohistochemical study in the rat indicated that glutamate release from axon terminals of projection fibers from the main and accessory olfactory bulbs might be regulated presynaptically and differentially through mGluR2/3, mGluR7a and/or mGluR8. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 根木 章夫: "Metabotropic glutamate receptor mGluR2 and mGluR5 are expressed in two non-overlapping populations of Golgi cells in the rat cerebellum" Neuroscience. 75・3. 815-826 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 李 雲慶: "Distribution of a GABAergic and glycinergic premotor neurons projecting to the facial and hypoglossal nuclei in the rat" The Journal of Comparative Neurology. 378・2. 283-294 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 李 金蓮: "Association of serotonin-like immunoreactives axons with nociceptive projection neurons in the caudal spinal trigeminal nucleus of the rat" The journal of Comparative Neurology. 384・1. 127-141 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 李 泰喜: "Preprodynorphin-,preproenkephalin-,and preprotachykinin-expressing neurons in the rat neostriatum" The Journal of Comparative Neurology. 386・2. 229-244 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木下 彩栄: "Immunohistochemical localization of metabotropic glutamate receptors,mGluR7a and mGluR7b,in the central nervous system of the adult rat and mouse" The Journal of Comparative Neurology. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 和田 英樹: "Metabotropic glutamate receptor subtypes in axon terminals of projection fibersfrom the main and accessory olfactory bulbs" The Journal of Comparative Neurology. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akio Neki: "Metabotropic glutamate receptor mGluR2 and mGluR5 are expressed in two non-overlapping populations of Golgi cells in the rat cerebellum" Neuroscience. 75-3. 815-826 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yun-Qing Li: "Distribution of a GABAergic and glycinergic premotor neurons projecting to the facial and hypoglossal nuclei in the rat" J.Comp.Neurol.378-2. 283-294 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jin-Lian Li: "Association of serotonin-like immunoreactive axons with nociceptive projection neurons in the caudal spinal trigeminal nucleus of the rat" J.Comp.Neurol.384-1. 127-141 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Teffy Lee: "Preprodynorphin-, preproenkephalin-, and preprotachykinin-expressing neurons in the rat neostriatum" J.Comp.Neurol.388-2. 250-264 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ayae Kinoshita: "Immunohistochemical localization of metabotropic glutamate receptors, mGluR7a mGluR7b, in the central nervous system of the adult rat and mouse" J.Comp.Neurol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Eiki Wada: "Metabotropic glutamate receptor subtypes in axon terminals of projection fibers from the main and accessory olfactory bullbs" J.Comp.Neurol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 李 金蓮: "Association of serotonin-like immunoreactive axons with nociceptive projection neurons in the caudal spinal trigeminal nucleus of the rat" The Journal of Comparative Neurology. 384・1. 127-141 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 李 泰喜: "Preprodynorphin-,preproenkephalin-,and preprotachykinin-expressing neurons in the rat neostriatum" The Journal of Comparative Neurology. 386・2. 229-244 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 李 泰喜: "Collateral projections from striatonigal neurons to substance p receptor-expressing intrinsic neurons in the striatum of the rat" The Journal of Comparative Neurology. 388・2. 250-264 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大石 仁: "Distribution of a metabotropic glutamate receptor,mGluR2,in the central nervous system of the rat and mouse" Neuroscience Research. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木下 彩栄: "Immunohistochemical localization of metabotropic glutamate receptors,mGluR7a and mGluR7b,in the central nervous system of the adult rat and mouse" The Journal of Comparative Neurology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 和田秀樹: "Metabotropic glutamate receptor subtypes in axon terminals of projection fibers from the main and accessory olfactory bulbs" The Journal of Comparative Neurology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 李 雲慶: "GABAergic and glycinergic neurons projicting to the trigeminal motor nucleus : a double labeling study in the rat." The Journal of Comparative Neurology. 373・4. 498-510 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 根岸章夫: "Metabotropic glutamate receptor mG1uR2 and mG1uR5 are exoressed in two nonoverlaooing populations of Golgi cellsin the rat cerebellum" Neuroscience. 75・3. 815-826 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 李 雲慶: "Distribution of GABAergic and glycinergic premotor neurons projicting to the facial and hypoglossal nuclei in the rat" The Journal of Comparative Neurology. 378・2. 283-294 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 李 金蓮: "Distribution of trigeminohypothalamic and spinohypothalamic tract neurons displaying substance P receptor-like immunoreactivity in the rat" The Journal of Compartive Neurology. 378・4. 508-521 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 李 和: "Localization of a metabotropic glutamate receptor mG1uR7, in axon terminals of presumed nociceptive, primary afferent fibers……" Neuroscience Letters. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 陳 軍: "The locus coeruleus of the Japanese monkey (Macaca fuscata) does not express μ-opioid receptor-like immuroreactivity" Brain Research. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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