• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

DIFFERENTIAL MECHANISM IN NERVE REGENERATION BETWEEN CNS AND PNS DIFFERENCE

Research Project

Project/Area Number 08458246
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionASAHIKAWA MEDICAL COLLEGE (1997)
Osaka University (1996)

Principal Investigator

KIYAMA Hiroshi  Asahikawa medical College, Professor, 医学部, 教授 (00192021)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsCNS / PNS / HYPOGLOSSAL / DIFFERENTIAL DISPLAY / NERVE INJURY / REGENERATION / GLUTAMATE TOXICITY / MOTONEURON / ディファレンシャルディスプレイ / 神経軸索再生 / サイクリンG / グルタミン合成酵素 / キネシン
Research Abstract

In order to identify nerve regeneration associated molecules, we have used a method called the differential display. We purified RNA from nerve injured-and normal hypoglossal nuclei respectively, and these RNAs were further processed for the differential display. The present method successfully demonstrated several candidate genes which were up-regulated after nerve injury. A screening using in situ hybridization histochemistry further squeeze the candidates. These are (1) molecules belonging to glutamate metabolism, (2) molecules implicated in free radical scavenger, (3) a certain group of growth factor receptors, (4) molecules along a certain intracellular signaling, and (5) molecules implicated in redox regulation. Since these molecules were derived from adult injured motoneurons which can survive even after nerve injury. We examined if these molecules were also respond to a similar type of nerve injury in new born motoneurons which were fated to die by nerve injury. Both adult and pups hypoglossal nerve were axotomized, and in situ hybridization was carried out to identify transcription regulations. In this comparison, we found that some molecules have an ability to respond to nerve injury in adult, but not in pups. For instance, LIF-R and GDNFR (alpha) respond to nerve injury positively in adult, but negatively in pups. This opposite responses to nerve injury may be a reason why adult motoneurons can survive and pup's motoneurons die after nerve injury.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yao, GL: "Enhancement in expression of cytokine receptor andits intracellular signaling molecules after peripheral nerve injury." Eur.J.Neurosci.9. 1047-1054 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Su, QN: "Differential display revealedtranscriptional upregulation of the motor molecules forboth anterograde and retrograde axonal transport during nerve regeneration." Eur.J.Neurosci.9. 1542-1547 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oshige-Hayashi Y: "Expression of Glycine max(soybean agglutinin: SBA)binding molecue in injured motoneurons andits specific localization in the extracellular matrix between injured neurons and microglia." Neurosci.Res.27. 271-275 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morita N,et al: "p53 independent cyclin G expression in a group of mature neurons and its enhanced expression during nerve regeneration." J.Neurosci. 16. 5961-5966 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirota H,et al.: "Accelelated nerve regeneration in mice by upregulated expression of IL-6 and IL-6 receptor after trauma." J.Exp.Med.183. 2627-2634 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morita N,et al.: "Distinct localization of two serine/threonine kinase receptors for activin and TGF-b in the rat brain and transient down-regulation of Type I activin receptor during peripheral nerve regeneration." Mol.Brain Res.42. 263-271 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yao GL,et al.: "Enhancement in expression of cytokine receptor and its intracellular signaling molecules afte peripheral nerve injury." Eur.J.Neurosci. 9. 1047-1054 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Su QN,et al.: "Differential display revealedtranscriptional upregulation of the motor molecules for both anterograde and retrograde axonal transport during nerve regeneration." Eur.J.Neurosci. 9. 1542-1547 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oshige-Hayashi Y,et al.: "Expression of Glycine max (soybean agglutinin : SBA) binding molecule in injured motoneurons and its specific localization in the extracellular matrix between injured neurons and microglia." Neurosci.Res.27. 271-275 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morita,N: "p53 independent cyclin G expression in a group of mature neurons and its enhanced expression during nerve regeneration." J.Neurosci.16. 5961-5966 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hirota,H: "Accelelatednerve regeneration in mice by upregulated expression of IL‐6 and IL‐6 receptor after trauma." J.Exp.Med.183. 2627-2634 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morita,N: "Distinct localization of two serine/threonine kinase receptors for activin and TGF‐b in the rat brain and transient down‐regulation of Typel activin receptor during peripheral nerve regeneration." Mol.Brain.Res.42. 263-271 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tao,GL: "Enhancementin expression of cytokine receptor and its intracellular signaling molecules after peripheral nerve injury." Eur.J.Neurosci.9. 1047-1054 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Su,QN: "Differential display revealedtranscriptional upregulation of the motor molecules for both anterograde and retrograde axonal transport during nerve regeneration." Eur.J.Neurosci.9. 1542-1547 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Oshige‐Hayashi,Y: "Expression of Glycine max(soybean agglutinin:SBA)binding molecule in injured motoneurons and its specific localization in the extracellular matrix betweeninjured neurons and microglia." Neurosci.Res.27. 271-275 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morita N: "Distinct localization of two serine/threonine kinase receptors for activin and TGF-β in the rat brain and transient down-regulation of Type1 activin receptor during peripheral nerve regeneration." Mol.Brain Res.42. 263-271 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Morita N: "p53 independent cyclin G expression in a group of mature neurons and its enhanced expression during nerve regeneration." J.Neurosci.16. 5961-5966 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yao GL: "Enhancement in expression of cytokine receptor and its intracellular signaling molecules after peripheral nerve injury." Eur.J.Neurosci.in press.(1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi