| Project/Area Number |
08458276
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
ESAKI Kozaburo Osaka Prefecture University Col.of Agric.Professor, 農学部, 教授 (70072402)
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| Co-Investigator(Kenkyū-buntansha) |
YAMATE Jyoji Osaka Prefecture University Col.of Agric.Assistant professor, 農学部, 講師 (50150115)
MORI Nobuko Osaka Prefecture University Advanced Science and Technology Research Associate, 先端科学研究所, 助手 (90100221)
OKUMOTO Masaaki Osaka Prefecture University Advanced Science and Technology Professor, 先端科学研究所, 教授 (50100186)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1996: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Hereditary cataract / Disease model / Microsatellite marker / Lens rupture / Linkage analysis / Gene mapping / Mouse / lr2 / Ir2 / 1r2 |
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| Research Abstract |
A new cataract model originated in a recombinant inbred (RI) strain, CXS4 or CXSD (D), between BALB/cHeA (BALB/c or C) and STS/A (STS or S) mice. Opacity appeared as a white pinpoint focus in unpigmented eyes of albino mice from 5 weeks old. All the mice were bilaterally affected by 14 weeks old. They were fully viable and fertile. There was no sex difference in incidence of cataract. Histologically, the 3-4 months old mice showed vacuoles in the lens cortex. The vacuoles were spread all over the lens cortex in advanced cases. Ruptures of the lens nucleus to the vitreous chamber was a typical occurrence. For elucidation of the mode of inheritance, F1 hybrids (CXD and SXD) and backcross progenies [(CXD) F1XD and (SXD) F1XD] were analyzed. NO affected mice were observed in F1 hybrids. In backcross progenies, the segregation ratio of affected and normal mice was close to 1 : 1 in both matings. We conclude that the cataract is inherited by an autosomal single recessive gene. This mutant gene is provisionally named 'lens rupture 2' (gene symbol 1r2, Mouse Genome Database Accession No.MGD-JNUM-37399).
Mapping of the spontaneous autosomal recessive cataract mutant gene was performed. For the linkage analysis, we produced two kinds of backcross progenies, (BALB/cHeA x D) F1 and (STS/A x D) F1 females crossed to D male mice. The gene (lr2, lens rupture2) was mapped to the central part of Chromosome (Chr) 14,0.7+/-0.7 cM from the micosatellite marker D14Mit28.
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