| Project/Area Number |
08458291
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kagoshima University |
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Principal Investigator |
BABA Masanori Kagoshima University Faculty of Medicine Professor, 医学部, 教授 (70181039)
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| Co-Investigator(Kenkyū-buntansha) |
KISHIDA Akio Kagoshima University Faculty of Engineering Associate Professor, 工学部, 助教授 (60224929)
MAKINO Masahiko Kagoshima University Faculty of Medicine Associate Professor, 医学部, 助教授 (60238889)
AKASHI Mitsuru Kagoshima University Faculty of Engineering Professor, 工学部, 教授 (20145460)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | HIV / Nanosphere / Lectin / Monnose / Capture / Prophylaxis |
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| Research Abstract |
HIV-1 gp120 has been shown to strongly interact with some lectins. Thus, gp120 and HIV-1 virions are expected to be effectively captured by lectins if they are immobilized to certain materials. Polymeric particles are often used as a material for immobilization of biomolecules such as antibodies and enzymes. Among them, polystirene particles are quite useful, since monodispersed particles can be easily prepared. Polystirene nano-spheres (NSs) covered with poly (methacrylic acid) were prepared by copolymerization of stirene with poly (tert-butylmethacrylayte) macromonomer and acid hydrolysis. Resulting NSs (mean diameter : 400nm) were dispersed well in water. The immobilization of concanavalin A (Con A) was performed by using water soluble carbodiimide. The surface of the obtained NSs (Con A-NSs) was found to be fully covered with Con A.The interaction of Con A-NSs with HIV-1 was determined by the reduction of gp120 level and viral infectivity of HIV-1 suspensions after 60-min incubation at room tem-perature. Con A-NSs achieved a>3.3 log and a 2.2 log reduction of viral infectivity in HIV-1 (III_B strain) suspension at a concentration of 2 and 0.5 mg/ml, respectively. Whereas Con A-free nanospheres, which were not immobilized with Con A,achieved only a 0.29 log reduction at 0.5 mg/ml. Con A-NSs (2 mg/ml) could also reduce the gp 120 level off III_B and HE strains to < 7.1 and 5.5% of each control, respectively. The combination of Con A-NS treatment followed by filtration with a microporous membrane efficiently removed virion-free gp120 as well as infectious viral particles from HIV-1 suspension. Scanning electron microscopy demonstrated that HIV-1 virions were trapped on the surface of Con A-NSs. Thus, Con A-NSs can capture HIV-1 virions and gp120 with a high affinity and may have potential as an effective tool for the prevention of HIV-1 transmission.
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