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Characterization of a signaling molecule assembly region within the cell membrane

Research Project

Project/Area Number 08459016
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionKyoto Institute of Technology

Principal Investigator

MAEKAWA Shohei  Kyoto Institute of Technology, Textile Science Associate Professor, 繊維学部, 助教授 (40173695)

Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordsneuronal raft / cytoskeleton / GP1-anchored proteins / paralemin / NAP-22 / cholesterol / calmodulin / GAP-43 / 神経 / Gタンパク質 / タンパク質キナーゼ / 細胞接着因子 / 情報伝達 / 脳 / GPI-アンカー蛋白質 / シナプス / チロシンリン酸化
Research Abstract

Characterization of a signaling molecule assembly region(raft) within the cell membrane in the brain tissue resulted in the following findings :
1. Major components were identified as, tubulin, actin, NAP-22, GAP-43, and several trimeric G proteins, using western blotting and partial amoni acid sequencing analysis.
2. Concentration of several GPI-anchoed proteins, such as F3/contactin, T-cadherin, OBCAM, and neurotrimin was found. In addition, a novel GPI-anchored protein was idenfied and cloned. This protein, named kilon, showed a wide distribution in the brain cortex.
3. Production of monoclonal antobodies using raft as an immunogen resulted in the identification of a protein focalized in raft. This protein was identified as paralemin from its amino acid sequence.
4. Lipid analysis of raft showed the enrichment of phosphatidylcholine and cholesterol in this fraction. Extraction of cholesterol using methy-cyclodextrin caused specific solubilization of NAP-22. Further studies showed the binding of NAP-22 to a cholestrol containing liposome.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 前川昌平 他: "Identification of NAP-22 and GAP-43(neuromodulin)as major protein components in a Triton insoluble low density fraction of rat brain." Biochim.Biophys.Acta. 1323. 1-5 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本幸稔 他: "Biochemical evidence for the presence of NAP-22, a novel acidic calmodulin binding protein, in the synaptic vesicles of rat brain." Neurosci.Lett.224. 127-130 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 飯野 哲 他: "Immunohistochemical localization of a novel acidic calmodulin binding protein, NAP-22, in the rat brain." Neuroscience. 発表予定. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 前川昌平: "脳・神経系細胞膜中の界面活性剤不溶性低比重画分" 神経化学. 36. 31-35 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 船津宣雄 他: "Characterization of a novel rat brain GPI-anchored protein(kilon), a member of the IgLON cell adhesion molecule family." J.Biol.Chem.発表予定.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 前川昌平: "ブレインサイエンス'97(佐藤昌康/編)" 講談社, 11 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa, S., Kumanogoh, H., Funatsu, N., Takei, N., Inoue, K., Endo, Y., Hamada, K., and Sokawa, Y.: "Identification of NAP-22 and GAP-43 (neuromodulin) as major protein components in a Triton insoluble low density fraction of rat brain." Biochimica Biophysica Acta. 1323. 1-5 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto, Y., Sokawa, Y., and Maekawa, S.: "Biochemical evidence for the presence of NAP-22, a novel acidic calmodulin ibnding protein, in the synaptic vesicles of rat brain." Neurosci.Lett.224. 127-130 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] lino, S., Kobayashi, S., and Maekawa, S.: "Immunohistochemical localization of a novel acidic calmodulin-binding protein, NAP-22, in the rat brain" Neuroscience. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Funatsu, N., Miyata, S., Kumanogoh, H., Shigeta, M., Hamada, K., Endo, Y., Sokawa, Y., and Maekawa, S.: "Characterization of a novel rat brain GP1-anchored protein (kilon), a member of the lgLON Cell adhesion molecule family" J.Biological Chemistry. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 飯野哲 他: "Immunohistochemical localization of a novel acidic calmodulin binding protein,NAP-22,in the rat brain." Neuroscience. (発表予定). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 船津宣雄 他: "Characterization of a novel rat brain GPI-anchored protein(kilon),a member of the IgLON cell adhesion molecule family." J.Biol.Chem.(発表予定). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 前川昌平, 他: "Identification of NAP-22 and GAP-43 (neuromodulin) as major protein components in a Triton insoluble low density fraction of rat brain." Biochimica Biophysica Acta. 1323. 1-5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 久保田真理, 他: "Localization of 2',5'-oligoadenylate synthetase and the enhancement of its acttivity with recombinant interferon-aA/D in the mouse brain." Anal.Embryol.195. 251-257 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山本幸稔, 他: "Biochemical evidence for the localization of NAP-22,a novel calmodulin binding protein,in the synaptic vesicles of rat brain." Neuroscience Letters. 224. 127-130 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 久保田真理, 他: "The target cells of injected type I interferons in mouse liver." J.Cytokine and Interferon Res.(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 上田哲生, 他: "Production of Immunoreactive 2',5'-oligoadenylate Synthase in p48-deficient Mice." J.Cytokine and Interferon Res.(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 前川昌平,他: "Identification of NAP-22 and GAP-43(neuromodulin)as major protein conponents in a Triton insoluble lowdensityfraction of rat brain." Biochimica Biophysica Acta. 1323. 1-5 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久保田真理,他: "Localization of 2′,5′-oligoadenylate synthetase and the enhancement of its acttivity with recombinant interferon-α A/D in the mouse brain." Anal.Embryol.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 山本幸稔,他: "Biochemical evidence of the localization of NAP-22,a novel calmodulin binding protein,in the synaptic vesicles of rat brain." Neuroscience Letters. (in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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