| Project/Area Number |
08508003
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | NATIONAL CARDIOVASCULAR CENTER RESEARCH INSTITUTE |
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Principal Investigator |
TSUJI Takayuki NATIONAL CARDIOVASCULAR CENTER RESEARCH INSTITUTE,DEPARTMENT, DIRECTOR, 実験治療開発部, 部長 (00075764)
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| Co-Investigator(Kenkyū-buntansha) |
DOHI Takeyoshi UNIVERSITY OF TOKYO, PORFESSOR, 工学部・精密機械工学科, 教授 (40130299)
YASUMIZU Mitsuhiko YAMANASHI MEDICAL SCHOOL,ASSOCIATE, PROFESSOR, 産婦人科, 助教授 (80107705)
FUJISATO Toshia NATIONAL CARDIOVASCULAR CENTER RESEARCH INSTITUTE, Researcher, 実験治療開発部, 室員 (60270732)
SADA Masaharu NATIONAL CARDIOVASCULAR CENTER RESEARCH INSTITUTE, CHIEF RESEARCHER, 実験治療開発部, 室長 (20162399)
MIYAWAKI Fujio NATIONAL CARDIOVASCULAR CENTER RESEARCH INSTITUTE, CHIEF RESEARCHER, 実験治療開発部, 室長 (50174222)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥42,200,000 (Direct Cost: ¥42,200,000)
Fiscal Year 1998: ¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 1997: ¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 1996: ¥15,000,000 (Direct Cost: ¥15,000,000)
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| Keywords | transgenic minipig / three-dimensional rigid endoscope / ova-holding device / computer-guided microinjector / microporous glass plate / gene engineering |
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| Research Abstract |
Aim of the project is to establish an efficient system of creating transgenic minipigs for heterogenic organ transplantation. We invented three-dimensional (3D) rigid endoscopes for obtaining less-invasively minipig ova from ovarium without laparotomy and an ova-holding device having 5 x 5 micropocket matrix made of microporous glass plate. The device can suck ova into micropockets having diameter of 150 micrometer with solution circulating across the plate fixed in the holder housing. Ova can be cultured in the device under controlled environments of appriate temperature, pH, and gas contents. GFP marked-human gene can be introduced into the nucleus with the computer guided microinjector. All the procedures can be monitored with the 3 D microscope and recorded with a video-cassette recorder. The cultured GFP- marked embryo can be selectively picked up and introduced laparoscopically into female minipig uterus less-invasively with the 3 D rigid endoscope. This system can create several ten times more efficiently a trangenic minipig than the conventional method which needs much skill and more female minipig sacrification.
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