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Development of Lactic Acid Fermentation Process Accompanied with Selective Product Separation

Research Project

Project/Area Number 08555195
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 反応・分離工学
Research InstitutionOita University

Principal Investigator

HANO Tadashi  Faculty of Engineering, Oita University, Professor, 工学部, 教授 (80038067)

Co-Investigator(Kenkyū-buntansha) HIRATA Makoto  Faculty of Engineering, Oita University, Associate Professor, 工学部, 助教授 (20264327)
SAKAI Kenji  Faculty of Engineering, Oita University, Associate Professor, 工学部, 助教授 (50205704)
MORIGUCHI Mitsuaki  Faculty of Engineering, Oita University, Professor, 工学部, 教授 (70026574)
MIURA Shigenobu  Musashino Chemical Laboratory Inc., Research Manager, 主任研究員
TAKANASHI Hirokazu  Faculty of Engineering, Oita University, Research Associate, 工学部, 助手 (40274740)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1996: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsBioreactor / Lactic Acid Fermentation / Extractive Fermentation / Electrodialysis / Membrane Reactor / Biodegradable Polymer / Polylactic Acid
Research Abstract

The fermentative production process of L-lactic and as a raw material of biodegradable polymer was developed by employing Lactobacillus rhamnosus and Rhizopus oryzae and the following results were obtained concerning the fermentation and products separation.
1) In the extractive fermentation with Lactobacillus rhamnosus, it became possible to remove completely the dissolved extractant which is toxic for microbes by using a cleaning column packed with cation exchange resin. The product inhibition, which severely retarded the fermentation under the initial glucose concentration of 100g/L, was suppressed with the present extractive fermentation system and fermentation proceeded satisfactorily.
2) In the electrodialysis fermentation with Lactobacillus rhamnosus, a continuous fermentation method utilizing the permeation of water was found to give a stable production of lactic acid for long term. By using a glucose sensor in a fermentor, it was possible to keep the concentration of glucose at low level and to increase the yield of lactic acid.
3) In the fermentation with Rhizopus oryzae, the addition of activated carbon to the fermentation broth was very effective in increasing the productivity and yield and obtaining clean broth which simplified the downstream processing.
4) The lactic acid production systems investigated in this project were evaluated. It was concluded that an extractive fermentation with Lactobacillus rhamnosus was suitable for a small-scale batch operation whereas an electrodialysis fermentation with the same bacteria was for a small-scale continuous operation. Rhizopus oryzae was suitable for a large-scale fermentation.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Y.Tong: "Extraction of Lactic Acid from Fermented Broth with Microporous Hollow Fiber Membranes"化学工学シンポジウムシリーズ65 Advances in Bioseparation Engineering 1996-1997. 151-155 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Hano: "Development of Extraction System for Lactic Acid from Fermentor"Proceedings of International Solvent Extraction Symposia. 407-415 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Tong: "Solvent Screening of Lactic Acid by Extractive Fermentation"Separation Science and Technology. 33. 1439-1453 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Matsumoto: "Extraction Rates of Amino Acids by an Emulsion Liquid Membrane with Tri-n-octylmethylammonium Chloride"Journal of Chemical Technology and Biotechnology. 73. 237-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Tong: "Extraction of lactic acid from fermented broth with microporous hollow fiber membranes"Journal of Membrane Science. 143. 81-91 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Tong, T. Hano et al.: "Extraction of lactic acid from fermented broth with microporous hollow fiber membranes."J. Membrane Science. 143. 81-91 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T. Hano, M. Hirata et al.: "Development of extraction system for lactic acid from fermentor"Proceedings of the International Solvent Extraction Symposia. Moscow. 407-415 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Tong, T. Hano et al.: "Solvent screening for production of lactic acid by extractive fermentation"Separation Science and Technology. 33. 1439-1453 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M. Matsumoto, T. Hano et al.: "Extraction rates of amino acids by an emulsion liquid membrane with tri-n-octylmethylammonium chloride"J. Chemical Technol. Biotechnol.. 73. 237-242 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Tong, T. Hano et al.: "Back extraction of lactic acid with microporous hollow fiber membrane"J. Membrane Science. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Tong.: "Extraction of Lactic Acid from Fermented Broth with Microporous Hollow Fiber Membranes" 化学工学シンポジウムシリーズ65 Advances in Bioseparation Engineering 1996-1997. 151-155 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Hano: "Development of Extraction System for Lactic Acid from Fermentor" Proceedings of International Solvent Extraction Symposia. 407-415 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Tong: "Solvent Screening of Lactic Acid by Extractive Fermentation" Separation Science and Technology. 33. 1439-1453 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Matsumoto: "Extraction Rates of Amino Acids by an Emulsion Liquid Membrane with Tri-n-octylmethylammonium Chloride" Journal of Chemical Technology and Biotechnology. 73. 237-242 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Tong: "Extraction of lactic acid from fermented broth with microporous hollow fiber membranes" Journal of Membrane Science. 143. 81-91 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.P.Tong: "Membrane extraction of lactic acid from fermented broth" Proceedings of APBioChEC 98. 2. 914-917 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Matsumoto: "Recovery of boric acid from wastewater by solvent extraction" Separation Science and Technology. 32. 983-991 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Hano: "Removal of phosphorus from wastewater by activated alumina adsorbent" Water Science and Technology. 35. 39-46 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Hano: "Development of boron recovery system from desulfurization plant wastewater" Proceedings of Regional Symposium on Chemical Engineering 97. 233-237 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.P.Tong: "Extraction of lactic acid from fermented broth with microporous hollow fiber membrane" J.Membrane Science. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.P.Tong: "Solvent screening for production of lactic acid by extractive fermentation." Separation Science and Technology. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Hano: "Recovery of boric acid from wastewater by solvent extraction" Separation Science and Technology. Voll.32 (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Hano: "Removal of phosphorus from wastewater by activated alumina adsorvent" Water Science and Technology. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 羽野 忠: "独立栄養細菌による窒素除去システムの開発" 化学工学. Vol.61. 136-137 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Hano: "Extraction kinetics of organic acids with tri-n-octyl phophine oxide" J.Chem.Tech.Biotechnol.Vol.67. 260-264 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Hano: "Extractive fermentation of lactic acid with R.oryzae and L.rhamnosus" Values Adding Through Solvent Extraction. Vol.2. 1447-1452 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Hano: "Recovery of phenols using liquid surfactant membranes prepared with newly synthesized surfactant" Separation Science and Technology. Vol.31. 107-124 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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