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Development of Electrical Manipulation of Single Cells Using Microelectrodes

Research Project

Project/Area Number 08555198
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物・生体工学
Research InstitutionTohoku University

Principal Investigator

MATSUE Tomokazu  Tohoku Univ.Grad.Sch.Eng., Assoc.Prof., 大学院・工学研究科, 助教授 (70173797)

Co-Investigator(Kenkyū-buntansha) SAIKI Hiroshi  Cent.Inst.Electr.Pow.Ind., Gen.Manager, 我孫子研究所, 部長
YOKOYAMA Hidekatsu  Inst.Life Support Tech., Chief Invest., 計測法開発部, 主任研究員 (10281619)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsMicroelectrode / Dielectrophoresis / Cell Manipulation / Single cell / Photosynthesis / Microelectroporation / Patterning / Membrane permeability / 電子移動 / 酸化還元 / 電子伝達鎖 / エレクトロポレーション / 細胞配列 / レドックス反応 / 誘電泳動
Research Abstract

1.Micropatterns with living cells were formed on glass substrates using negative dielectrophoretic force induced at a template microarray electrode consisting of assembly of microband electrodes. The template electrode was placed above the substrate and cell suspension was sucked into the space between the substrate and electrode. When an ac voltage (typically, 7Vrms, 10kHz) was applied to the template electrode, the induced dielectrophoretic force repelled the cells from the electrode and pushed at the glass substrate to form the cell pattern which reflected the electrode shape.
2.Microelectroporation capillary for injection of genes or drugs into single cells were fabricated and the performance was investigated. When an electric pulse was applied, Fe (CN) ^3-_ dissolved in the solution inside the capillary was released by electromigration. The amount of the released Fe (CN) ^3-_ can be controlled by adjusting the magnitude and period of the pulse. We applied the poration capillary to … More inject Fe (CN) _6^<3-> into a single protoplast. The application of a electric pulse brought about a reversible membrane breakdown to form small pores in the membrane and simultaneously repelled Fe (CN) _6^<3-> from the capillary by electromigration.
3.Permeation of several redox species through a cell membrane of a single algal protoplast was investigated by amperometry with a Pt microdisk electrode located near the membrane. Permeability coefficient (Pm) of the protoplast membrane was determined by the quantitative analysis of the variation of the redox current with microelectrode-membrane distance using digital simulation.
4.Miceoamperometric measurements of a single protoplast were applied for quantitative investigation of the influence of p-benzoquinone (BQ) on the photosynthetic electron-transport. From the analyzes of the localized concentrations of BQ and p-hydroquinone (QH_2) , the consumption of BQ and the generation of QH_2 by a single protoplast upon light irradiation were determined. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 松本 伯夫: "単一細胞を対象としたマイクロエレクトロポレーションの開発" 電気学会論文誌. 116E. 184-189 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 末永 智一: "生体機能材料の局所センシング" 信学技報. 97. 17-21 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 末永 智一: "細胞一個の内部を電極で探る" 科学と教育. 45. 320-321 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 末永 智一: "マイクロ電極の特徴と応用" 科学工学. 61. 104-107 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsue: "Rapid Micropatterning of Livining Cells by Repulsive Dielectrophoretic force" Electrochim.Acta. 42. 3251-3256 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 末永 智一: "走査型電気化学顕(SECM)による局所センシング" 化学センサ. 13. 8-13 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasykawa: "Microamperometric Determination of Photosynthetic Oxygen Generation from aSingle Protoplast" Denki Kagaku,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasykawa: "Permeation of Redox Species Through a Cell Membrane of a Single,Living Algal Protoplast Studied by Microamperometry" Biochim.Biophys.Acta.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 末永 智一: "マイクロ電極システムを用いた細胞操作とセンシング" 応用物理. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Matsumoto, H.Saiki, T.Matsue, I.Uchida: "Development of Electroporation System for Single Cells" T.IEE Japan. 116-E. 184-189 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsue: "Microsensing of Biofunctional Materials" Tech.Rep.IEICE. 97. 17-21 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsue: "Exploration of Inside of a Single Cell" Kagaku to Kyoiku. 45. 320-321 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsue, I.Uchida: "Characteristics and Applications of Microelectrodes" Kagaku Kogaku. 61. 104-107 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsue, N.Matsumoto, I.Uchida: "Rapid Micropatterning of Living Cells by Repulsive Dielectrophoretic Force" Electrochim Acta. 42. 3251-3256 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsue: "Localized Sensing with Scanning Electrochemical Microscopy (SECM)" Chemical Sensors. 13. 8-13 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasukawa, I.Uchida, T.Matsue: "Microamperometric Determination of Photosynthetic Oxygen Generation from a Single Protoplast" Denki Kagaku. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasukawa, I.Uchida, T.Matsue: "Permeation of Redox Species Through a Cell Membrane of a Single, Living Algal Protoplast Studied by Microamperometry" Biochim.Biophys.Acta. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsue, T.Yasukawa, I.Uchida: "Manipulation and Characterization of Living cells Using Microelectrode Systems" Oyo Butsuri. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Shiku: "Dual lmmunoassay of Human Chorionic Gonadotropin and Human Placental Lactogen at a Microfabricated Substrate by Scanning Electrochemical Microscopy" J.Electroanal.Chem.438. 187-190 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 末永智一: "走査型電気化学顕(SECM)による局所センシング" 科学センサ. 13. 8-13 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Matsue: "Rapid Mecropatterning of Living Cells by Repulsive Dielectrophoretic force" Electrochim.Acta. 42. 3251-3256 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 末永智一: "細胞一個の内部を電極で探る" 化学と教育. 45. 320-321 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Yasukawa: "Permeation of Redox Species Through a Cell Membrane of a Single, Living Algal Protoplast Studied by Microamperometry" Biochim.Biophys.Acta,. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 末永智一: "マイクロ電極システムを用いた細胞操作とセンシング" 応用物理. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 松本伯夫: "単一細胞を対象としたマイクロエレクトロポレーションの開発" 電気学会論文誌. 116E. 184-189 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Shiku: "Detection of Microspotted Carcioembryonic Antigen on a Glass Substrate by Scanning Electrochemical Microscopy" Anal.Chem.68. 1276-1278 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Shiku: "Dual Immunoassay of Human Chorionic Gonadotropin and Human Placental Lactogen at a Microfabricated Substrate by Scanning Electrochemical Microscopy" J.Electroanal.Chem.(印刷中).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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