| Project/Area Number |
08555199
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物・生体工学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGAMUNE Teruyuki Univ.Tokyo, Dept.Chem.& Biotechnol., Professor, 大学院・工学系研究科, 教授 (20124373)
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| Co-Investigator(Kenkyū-buntansha) |
KITAYAMA Atsushi Univ.Tokyo, Dept.Chem.& Biotechnol., Resesarch Associate, 大学院・工学系研究科, 助手 (70270882)
UEDA Hiroshi Univ.Tokyo, Dept.Chem.& Biotechnol., Lecturer, 大学院・工学系研究科, 講師 (60232758)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | Homogeneous immunoassay / Fluorescence Resonance Energy Transfer / Transformation of Luminescence Wavelength / Chimeric Protein |
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| Research Abstract |
To develop a sensitive homogeneous immunoassay, verification of a homogeneous immunoassay principle which utilize luminescence energy transfer and transformation of luminescence wavelength, was attempted.
When variable amount of fluorescent labeled antibody was mixed with chimeric protein A-Vargula luciferase and substrate luciferin, marked red shift in luminescence spectre possibly due to efficient luminescence energy transfer was detected. It was also detectable with a microtiter well-type luminometer with long pass filter unit. The amount of labeled antibody correlated well with the extent of luminescence with red-shifted wavelength, which suggests the feasibility of new luminescent homogeneous immunoassay. To obtain better sensitivity in the assay, a novel chimeric protein of protein G-Vargula luciferase was made and shown to have 2-fold sensitivity than protein A-luciferase. The result thus implies a possibility of new antigen detection method employing epitope-tagged luciferase and fluorescent-labeled antibody.
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